Expression of Growth Differentiation Factor-9 from buffalo follicular fluid – a marker gene for fecundity

K. Muralidharan; S. Eswari; K. Vijayarani

doi:10.56093/ijvasr.v49i2.126846

Authors

K. Muralidharan M.V.Sc Scholar, Department of Veterinary Physiology, Madras Veterinary College, Chennai- 600 007
S. Eswari Professor, Department of Veterinary Physiology, Madras Veterinary College, Chennai- 600 007
K. Vijayarani Professor and Head, Bioinformatics and ARIS cell,Madras Veterinary College, Chennai - 600 007

https://doi.org/10.56093/ijvasr.v49i2.126846

Keywords:

Buffalo, Follicular fluid, GDF-9 expression, RT-PCR

Abstract

Growth factors synthesized from mammalian oocytes popularly known as Oocyte Secreted Factors (OSFs) play numerous role in ovarian folliculogenesis. Growth differentiation factor-9 (GDF- 9) produced within the ovary plays an essential role during follicle maturation through their actions on granulosa cells, but their expression in follicular fluid has not been studied. The purpose of this study was to detect the temporal expression pattern of GDF-9 from the follicular fluid of buffalo ovary, which may be correlated with the oocytes and embryo quality. The sensitive messenger ribonucleic acid detection of GDF-9 from follicular fluid was determined by Reverse Transcriptase-Polymerase Chain Reaction using bovine oligonucleotide primers. Our finding showed the qualitative detection of GDF-9 mRNA transcripts from follicular fluid of buffalo ovary.

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