Effect of exogenous enzymes supplementation on growth performance and histo morphology of duodenum of broilers fed cashew apple waste  based diets*

P.Venkatramana; S. Senthil Murugan; H.S. Patki

doi:10.56093/ijvasr.v48i1.130367

Authors

P.Venkatramana M.V.Sc Student, Department of Animal Nutrition, College of Veterinary and Animal Sciences, KVASU, Pookode - 673 576, Wayanad, Kerala
S. Senthil Murugan Assistant Professor,Department of Animal Nutrition, College of Veterinary and Animal Sciences, KVASU, Pookode - 673 576, Wayanad, Kerala
H.S. Patki Assistant Professor, Department of Veterinary Anatomy and Histology, College of Veterinary and Animal Sciences, KVASU, Pookode - 673 576, Wayanad, Kerala

https://doi.org/10.56093/ijvasr.v48i1.130367

Keywords:

Cashew apple waste, duodenum, enzymes, goblet cell, villus

Abstract

The current experiment was conducted to study the histology of duodenum in broilers fed with different levels of inclusion of cashew apple waste (CAW) as well as enzymes. The study was carried out for a period of 42 days. A sum total of two hundred and ten day-old
vencobb-400 broiler chicks were randomly divided into seven groups with three replicates of 10 chicks in each group. Group 1 (G1) received control diet prepared as per BIS (2007) recommendations. G2 and G3 birds received diet prepared with 5 and 10 per cent CAW
without any enzyme supplementation, respectively. G4 and G5 birds received 5 per cent CAW with supplementation of 500 g/ton and 750 g/ton of enzymes, respectively. G6 and G7 birds received 10 per cent CAW with supplementation of 500 g/ton and 750 g/ton of
enzymes, respectively. It was found that, histomorphological features like villus height and thickness of tunica mucosa were found to be higher in group (G4) where 5 per cent CAW with NSP degrading enzymes at 500 g/ton were fed significantly (p<0.01) when compared to all other groups. The number of goblet cells was observed to be significantly (p<0.01) lesser in the group (G4) when compared to all other groups.

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References

Balamurugan, R., Chandrasekaran, D. and Kirubakaran, A. (2011). Effects of multi-enzyme supplementation on gut morphology and Histomorphology in broilers. Indian J. Sci. and Tech. 4: 1518

Bhamare, K.S., Dildeep, V., Senthil Murugan, S. and Chavan, S.J. (2016). Nutritive evaluation of cashew apple waste in broilers. Intl. J. Sci. and

Nat, 7: 629-632

Bharathidhasan A., Chandrasekaran D., Natarajan A., Ravi R and Ezhilvalavan S. (2010). Effect of enzyme supplementation to nutrient reduced

diet on performance of broilers. Tamilnadu J. Vet. & Anim. Sci. 6: 174-178.

Choct, M. (2006). Enzymes for the feed industry: past, present and future. World’s Poul. Sci, 81: 1842-1849

Indian standard for Poultry Feeds, (2007). Bureau of Indian Standards (IS: 1374), 5th revision, New Delhi.

Luna, L.G. (1968). Mannual of Histologic Staining Methods of Armed Forces Institute of Pathology. (3rd Ed.). McGraw Hill Co., New York. 258

Luo, D., Yang, F., Yang, X., Yao, J., Shi, B. and Zhou, Z. (2009). Effects of xylanase on performance, blood parameters, intestinal morphology, microflora and digestive enzyme activities of broilers fed wheat-based diets. Asian-Aust J. Anim. Sci, 22: 1288-95.

Nian, F., Y. M. Guo, Y. J. Ru, F. D. Li, and A. Péron. (2011). Effect of exogenous xylanase supplementation on the performance, net energy and gut microflora of broiler chickens fed wheat-based diets. Asian Australas. J. Anim. Sci, 24: 400-406.

Mazhari, M., Golian, A and Kermanshahi, H. (2015). Effect of corn replacement with graded levels of wheat screening and enzyme supplementation on performance, blood lipids, viscosity and jejunal histomorphology of finisher broilers. Spanish J. Agric. Res.13

Murugan, S.S., Sudhis, K., Rajan, J.K., Varshney, L. and Virendra, K. (2015). Cashew apple (Anacardiumoccidentale): Evaluation of physical and chemical composition. Ind. J. Nat. Sci. 5(29): 4255-4259.

Panda., A.K., Ramarao., S.V., Raju., M.V.L.N. and Sharma., S.R. (2006). Dietary supplementation of probiotic Lactobacillus sporogenes

on performance and serum biochemicolipid profile of broiler chickens. Indian Journal of Poultry Science, 43: 235-240. ProgRes® capture 2.8.8.version. JENOPTIKR, Optronics.

Smirnov, A., Tako, E., Ferket, P.R. and Uni, Z. (2006). Mucin gene expression and mucin content in the chicken intestinal goblet cells are affected by in ovofeeding of carbohydrates. Poult. Sci. 85: 669-673.

Snedecor, G.W. and Cochran, W.G. (1989). Statistical Methods, (8th Ed.). Iowa State University, Ames, I.A.

Thavasiappan, V., Selvaraj, P., Jayachandran S. Visha P., Paramasivan S., Naik. B.R. (2016). Influence of enzyme supplementation on intestinal

morphology in Broiler Chicken. J. Agric. Vet. Sci, 9: 25-28

Venkatramana, P., Senthil Murugan, S., Shyama, K., Biju Chacko, Patki, H.S. and Sunanda. Qualitative and Quantitative Analysis of PhytoChemicals in Cashew Apple Waste. Indian. J. Nat. Sci, 8: 976-997

Yuan, J., Yao, J., Yang, F., Yang, X., Wan, X., Han, J., Wang, Y., Chen, X., Liu, Y., Zhou, Z. and Zhou, N. (2008). Effects of supplementing different levels of a commercial enzyme complex on performance, nutrient availability, enzyme activity and gut morphology of broilers. Asian Aust. J. Anim. Sci, 21: 692.

Zhang, L., Xu, J., Lei, L., Jiang, Y., Gao, F. and Zhou, G.H. (2014). Effect of xylanase supplementation on growth performance, nutrient digestibility and non-starch polysaccharides degradation in different sections of the gastrointestinal tract of broilers fed wheat-based diets. Asian Aust. J. Anim. Sci, 27: 855-861.