VP2 Gne based Molecular Characterization of Blue tongue Virus  Serotype 16 Isolated in 2017 from Andhra Pradesh, India

P.Kalyanil; Y.Narshimma Reddy; B.Bhagyalakshmi; G.Naresh; B.Sushmitha

doi:10.56093/ijvasr.v48i4.130571

Authors

P.Kalyanil Research scholar, Department of Veterinary Microbiology and Veterinary Biotechnology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, India
Y.Narshimma Reddy Assistant Professor, Department of Veterinary Microbiology and Veterinary Biotechnology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, India
B.Bhagyalakshmi Assistant Professor, Department of Veterinary Microbiology and Veterinary Biotechnology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, India
G.Naresh Assistant Professor, Department of Veterinary Microbiology and Veterinary Biotechnology, College of Veterinary Science, PVNRTVU, Rajendranagar, Hyderabad-500030, India
B.Sushmitha Ella Foundation, Shamirpet, Hyderabad-500078, India

https://doi.org/10.56093/ijvasr.v48i4.130571

Keywords:

Bluetongue, serotype-16, VP2, topotype

Abstract

Bluetongue virus (BTV) genome consists of 10 segmented double-stranded ribonucleic acid (dsRNA). Of these, genome segment 2 (Seg-2), which encodes outer capsid protein VP2 is the primary determinant of virus serotype. In the present study, partial VP2 gene of KRL2/2017 (BTV-16) isolate collected from Kurnool district of Andhra Pradesh was amplified to know the evolutionary relationship of this isolate with previously reported Indian and global BTV-16 isolates. Seg-2 sequence data of KRL2/2017, showed >98% nucleotide identity with other Indian isolates. Comparison of this isolate with BTV-16 isolates across the world, showed higher sequence homology to isolates of Japan followed by Greece and China than to those of isolates from South Africa, Italy, UK, Australia and Indonesia. Phylogenetic analysis suggest that the isolate of the current study belong to eastern topotype similar to the viruses recovered from Japan, Greece and China reflecting their common eastern origin.

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