EffECT Of MULTI ENZYME SUPPLEMENTATION ON ILEAL DIGESTIBILITIES Of DRY MATTER, PROTEIN, ETHER EXTRACT, PHYTATE PHOSPHORUS AND NON-STARCH POLYSACCHARIDES IN BROILER DIET
Keywords:
Enzyme supplementation, ileal digestibility and nutrientsAbstract
A biological trial was conducted to study the effect of multi enzyme supplementation on ileal digestibilities of dry matter (DM), crude protein (CP), ether extract (EE), phytate phosphorus (PP) and non-starch polysaccharides (NSPs) in terms of neutral detergent fibre (NDF), acid detergent fibre (ADF), hemicellulose and cellulose in broilers for a period of 0-6weeks. The broilers used in this trial were fed diets supplemented with enzyme at 0, 250, 500, 750 and 1000 g/ton of feed with a proportionate reduction in metabolizable energy (ME-1.25, 2.5, 3.75 and 5 %), crude protein (CP-0.75, 1.5, 2.25 and 3 %), methionine + cytine (0.5, 1, 1.5 and 2 %) and available phosphorus (2.2, 4.4, 6.6 and 8.8 %). The level of individual enzyme per gram of multi enzyme supplement was cellulase 146 IU, xylanase 241 IU, pectinase 98 IU, protease 74 IU, amylase 778 IU and phytase 33 IU. The ileal digestibility was conducted on 6 th week of trial. The ileal DM digestibility (%) was significantly (p<0.01) increased in 750 g and 1000 g enzyme supplemented groups than other treatment groups. The increase in ileal DM digestibility was significantly (p<0.01) higher (6.2 %) in the group fed with 750 g enzyme supplementation than control. The digestibility of ileal crude protein (%) significantly (p<0.01) increased in 500, 750 and 1000 g enzyme supplemented groups than control. The increase in ileal protein digestibility was 12.20 % in 500 g/ton, 9.90 % in 750 g/ton and 10.12 % in 1000 g enzyme supplemented groups than control. However a non significant difference was observed in ileal EE digestibility in all treatment groups. The ileal phytate phosphorus digestibility significantly (p<0.01) increased in all the enzyme supplemented groups (33.69 % in 250 g/ton, 38.75 % in 500 g/ton, 39.53% in 750 g/ton and 42.11% in1000 g/ton) than control (31.71%). A highly significant (P< 0.01) increase in ileal neutral detergent fibre (NDF) and hemicellulose was observed in 500 to 1000 g enzyme supplemented groups than control. Similarly significant (P<0.01) increase in ileal acid detergent fibre (ADF) and cellulose digestabilities (%) were observed in all enzyme supplemented groups than control. It was concluded that the multi enzyme supplementation at minimum inclusion level of 500 g per ton of feed increased the ileal digestibilities of CP, PP, NDF, ADF, hemicelluloses and cellulose by 12.20 %, 18.17 %, 13.57 %, 55.63 % 12.77 % and 52.64 % respectively than control in broiler diet than the control diet in broilers.
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Allen, C.M., K.J. Mc Cracken and M.R. Bedford, 1997. Effect of fat type, rate of inclusion and enzyme supplementation on diet metabolizability and broiler performance. Br. Poult. Sci., 38: S25-S26.
Alloui, O., Chibowska, M and Smulikowska,S. 1994. Effects of enzyme supplementation on the digestion of low glucosinolate rapeseed meal in vitro, and its utilization by broiler chicks. J. Anim. and Feed Sci., 3 (2): 119-128.
AOAC, 2007. Official Methods of Analysis, Association of Official Analytical Chemists, 18th edn.,Washington, D.C.
Baidoo, S.K., Y.G. Lui and D. Yungbiut, 1998. Effect of microbial enzyme supplementation on energy, amino acid digestibility and performance of pigs fed hulls barley based diets. Can. J. Anim. Sci., 78: 625- 631.
Bharathidhasan, A., D. Chandrasekaran, A. Natarajan, R. Ravi and S. Ezhilvalavan, 2009. Effect of enzyme supplementation on digestibility and metabolizability of broilers to nutrient reduced diet. Indian Journal of Animal Research, 43(2): 79-83, 2009
Bharathidhasan, D Chandrasekaran, A Natarajan, R Ravi and S Ezhilvalavan, 2010. Effect of enzyme supplementation on performance of broilers to nutrient reduced diet, Tamil Nadu Journal Veterinary and Animal Science, 6(4): 174-178
BIS. 1992. Requirement for chicken feeds. IS:1374 – 1992, Manak Bhavan, 9 Bahadurshah Zafar Marg, New Delhi-110001.
Carlos,A.B. and Edwards, H.M.J.R.1998. The effects of 1,25 dihydroxy cholecalciferol and phytase on the natural phytate phosphorus utilisation by laying hens. Poult. Sci., 77: 850-858.
Danicke, S., O. Simon, H. Jeroch and M. Bedford, 1997. Interactions between dietary fat type and xylanase supplementation when rye based diets are fed to broiler chicken. 2. Performance, nutrient digestibility and the fat soluble vitamin status of livers. Br. Poult. Sci., 38: 546-556
Edwards, Hardy M.J.R. 1993. Dietary 1, 25- Dihydroxy cholecalciferol supplementation increases natural phytate phosphor utilization of chickens. J. Nutr., 123: 567-577.
Friesen, O.D., Guenter,W. Marquardt, R.R. and Rotter, B.A. 1992. The effect of enzyme supplementation on the apparent metabolizable energy and nutrient digestibility of wheat, barley, oats and rye for the young broiler chick. Poult.Sci., 71:1710-1721.
Glamocic, D., M. Polovinski-Horvatovic, M. Ivkovic, D. Beukovic, S. Bjedov, 2011. Effects of enzymes supplementation on digestibility and energy utilizations of broilers diets with different metabolizable energy level, Biotechnology in Animal Husbandry 27 (3) ; 583-590.
Goering, H.K. and Vansoest, P.J. 1970. Forage Analysis. Agriculture Hand book No.379. Agricultural Research Service, U.S.D.A., Bethesda, Washington. D.C. 1-20.
Haugh, Wolfgang and Hans-Joachim Lantzsh, 1993. Sensitive method for the rapid determination of phytate in cereals and cereal products. J. Sci. Food. Agri., 34: 1423-1426.
Khattak, F.M., T. N. Pasha, Z. Hayat and A. Mahmud, 2006. Enzymes in poultry nutrition. J.Anim. Plant Sci., 16: 1 – 7.
Langhout, D.J., Schutte, J.B. Geerse, C. Kies, A.K. De Jong, J and Verstegen, M.W.A. 1997. Effects on chick performance and nutrient digestibility of an endo-xylanase added to a wheat and rye based diet in relation to fat source. Br. Poult. Sci., 38: 557-563.
Leeson, S. And J.D. Summers, 2005. Commercial Poultry Nutrition. 3rd ed. Guelph: University Books.
Manoj Sharma. and Katoch, B.S. 1993. Supplementation of Novozyme SP243 in the diet of layers having low fibre content. Indian J. Anim.Nutr., 10(4): 247-250.
Saki, A.A., M.T. Mazugi and A. Kamyab, 2005. Effect of Mannanase on Broiler Performance, Ileal and In-vitro Protein Digestibility, Uric Acid and Litter Moisture in Broiler Feeding, International Journal of Poultry Science 4 (1): 21-26
Selle, P. H., V. Ravindran and G. G. Partridge. 2009. Beneficial effects of xylanase and/or phytase inclusions on ileal amino acid digestibility, energy utilisation, mineral retention and growth performance in wheat based broiler diets. Anim. Feed. Sci. Technol.153: 303–313.
Shim, Young. Ho, Jin Soo Kim, Santosh Laxman Ingale, Abdolreza Hosseindoust, Piyush Chandrakant Rathi, Yo Han Choi, Min Ju Kim, Alip Kumar, Seung Min Oh and Byung Jochae, 2017. Supplementation of pHS table Multienzyme Improved Growth Performance of Broiler Chickens. Asian J. Poult. Sci., 11(2):75-82
Short, F.J., P. Gorton, Wiseman, K.N. Boorman, 1996. Determination of titanium dioxide added as a inert marker in chicken digestibility studies. Anim. Feed Sci. and Tech., 59: 215221.
Simons, P.C.M. and Versteegh, H.A.J. 1990. Improvement of phosphorus availability by microbial phytase in broiler and pigs. Br. J. Nutr., 64: 525-540.
Slominski, Bogden A. and Campbell, C.D. 1990. Non starch polysaccharide of canola meal, quantification, digestibility in poultry and potential benefit of dietary enzyme supplementation, J.Sci. Food Agric., 53: 175-184.
Snedecor, G.W. and Cochran, W.C.1989. Statistical Methods 8th edn. Iowa State University Press, Ames, Iowa.
Steenfeldt, S and D. Pettersson, 2001. Improvements in nutrient digestibility and performance of broiler chickens fed a wheat-and rye based diet supplemented with enzymes Journal of Animal and Feed Sciences, 10: 143 - 157
Truonga, Ha. H., Shukun Yuc, Amy F. Mossa, Gary G. Partridged, Sonia Y. Liua, Peter H. Sellea, 2017. Phytase inclusions of 500 and 2000 FTU/kg in maize-based broiler diets impact on growth performance, nutrient utilisation, digestive dynamics of starch, protein (N), sodium and phytate degradation in the gizzard and four small intestinal segments Anim. Feed. Sci. Technol. 223: 13-22.
Zanella, I., N.K. Sakomura, F.G. Silversides, A. Fiqueird, and M. Pack, 1999. Effect of enzyme supplementation of broiler diets based on corn and soybeans. Poult. Sci., 78: 561-568.
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