FUNCTIONAL ANALYSIS OF CUMULUS CELLS ASSOCIATED GENES RELATED TO THE QUALITY OF IN VITRO FERTILIZED  CAPRINE EMBRYOS

M. Elanchezhian; S. Gautham; D. Reena; D. Gopikrishnan; A. Palanisammi

doi:10.56093/ijvasr.v47i4.137996

Authors

M. Elanchezhian Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai – 600 007
S. Gautham Centralized Embryo Biotechnology Unit, Department of Animal Biotechnology, TANUVAS, Madhavaram Milk Colony, Chennai-51
D. Reena Assistant Professor, Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai – 600 007
D. Gopikrishnan Resident Veterinary Services Section, Madras Veterinary College, TANUVAS, Chennai – 600 007
A. Palanisammi Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai – 600 007

https://doi.org/10.56093/ijvasr.v47i4.137996

Keywords:

Oocytes, Cumulus cells, Gene expression, Potential markers, Caprine embryos

Abstract

This study was undertaken to identify the expression of certain genes in cumulus cells as a potential marker for the oocyte quality. Grade A and grade B cumulus oocyte complexes (COCs) were matured and cumulus cells were isolated whereas the oocytes with partial cumulus cells were fertilized. The cleavage rates of grade A oocytes (44.29±1.31) were compared with the cleavage rates of grade B oocytes (34.83±2.72). The developmental rate differences were correlated with the expression of genes (COX2, HAS2, & GREM1) from the cumulus cells of grade A and grade B oocytes. The present result showed that the above said genes express in both grade A and grade B oocytes, irrespective of quality, directing the study towards the quantification of these genes which might bring out a concrete theory behind the mechanism and physiology of the differential expression of these genes in cumulus cells and their feedback on embryo quality.

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