STRONTIUM CHLORIDE INDUCED PARTHENOGENETIC OOCYTE ACTIVATION AND EMBRYONIC DEVELOPMENT IN BUFFALOES

D. Reena; S. Balasubramanian; D. Gopikrishnan; A. Palanisammi

doi:10.56093/ijvasr.v45i3.147388

Authors

D. Reena Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai – 600 007
S. Balasubramanian Department of Animal Reproduction Gynaecology and Obstetrics, Madras Veterinary College, TANUVAS, Chennai-600 007
D. Gopikrishnan Resident Veterinary Services Section, Madras Veterinary College, TANUVAS, Chennai – 600 007
A. Palanisammi Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai – 600 007

https://doi.org/10.56093/ijvasr.v45i3.147388

Keywords:

Activation, Strontium chloride, 6-Dimethylaminopurine, Embryo development, Buffalo

Abstract

Artificial oocyte stimulation is essential for assisted reproductive technologies like Somatic Cell Nuclear Transfer (SCNT), Intracytoplasmic Sperm Injection (ICSI). In this study, we examined the ability of strontium Chloride (Sr2+) to induce activation and in vitro embryonic development in buffalo oocytes in combination with Cytochalasin B and 6- dimethyl aminopurine (DMAP). Matured buffalo oocytes (n=276) were activated with 10 µM Sr2+ and 5 µg/ml cytochalasin B (Group I) for 2.5 hrs. In group II, 266 matured oocytes were activated with 10 µM Sr2+ and 5 µg/ml cytochalasin B for 2.5 hrs followed by culture in 2 mM 6-DMAP for 4 hrs. The activation rate for group I was significantly higher (P < 0.01) than for group II (38.35±1.00% and 31.29±0.90%, respectively). However, no significant difference was observed in morula development (9.57±0.84% and 6.80±0.59%, respectively). In conclusion, it is practicable to use Sr2+ in combination with cytochalasin B for parthenogenetic embryo production in buffalo.

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