IN VITRO VALIDATION OF SEX SORTED BULL SEMEN PRODUCED USING FLOW CYTOMETRIC METHOD

S. Rangasamy; S. Balasubramanian; G. Dhinakar Raj; A. Palanisammi; D. Reena; R. Chitra

doi:10.56093/ijvasr.v53i2.152726

Authors

S. Rangasamy Associate Professor and Head, Dept. of Veterinary Gynaecology and Obstetrics, VCRI, Theni – 625 534
S. Balasubramanian Former Director of Clinics, TANUVAS, Chennai-51
G. Dhinakar Raj Former Professor and Head, Dept. of Animal Biotechnology, MVC, Chennai-7
A. Palanisammi Former Dean, Veterinary College and Research Institute, Tirunelveli - 627 358
D. Reena Associate Professor, Dept. of Veterinary Clinical Complex, Veterinary College and Research Institute, Udumalpet – 642 126
R. Chitra Assistant Professor and Scientist, Translational Research Platform for Veterinary Biologicals, MMC, Chennai-51

https://doi.org/10.56093/ijvasr.v53i2.152726

Keywords:

Bull, Sex sorted semen and flow cytometry

Abstract

A commercially procured flow cytometrically sorted semen was reanalyzed to validate the effect of flow cytometry on structural and functional viability of spermatozoa. Flow cytometric analysis of commercially procured sex sorted frozen thawed bull semen showed the percentage of X sperms to be 95.31± 0.21 and Y sperms to be 4.69 ± 0.27, respectively. As expected sexed sperm had lower post thaw motility than the non-sexed sperm. However, no significant differences in the percentage of sperms with intact acrosome and expression of ROS were observed. Interestingly, DNA fragmentation index was statistically lower for the commercially procured sorted than for unsorted frozen thawed bull semen.

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References

Agarwal, A. and Said, T.M. (2009). Sperm chromatin Assessment. In textbook of Assisted Reproductive Techniques: Laboratory and clinical perspectives II Edn. Inform Healthcare, London, Pp, 93 - 106.

Ball, B.A. (2008). Oxidative stress, osmotic stress and apoptosis: impacts on sperm function and preservation in the horse. Animal Reproduction Science, 107: 257 – 267.

Biswas, J., Chakrabarti, A., Saha, K., Das, U., Pal, M., Pathak, P., Misra, S. and Mandal, A. (2013). Flow cytometric sorting of Sahiwal bulls semen coupled with artificial insemination at organized herd. Indian Journal of Animal Sciences, 83: 1275 - 1278.

Boe-Hansen, G.B., Ersboll, A.K. and Christensen, P. (2005). Variability and laboratory factors affecting the sperm chromatin structure assay in human semen. Journal of Andrology, 26: 360 – 368.

Bordignon, V. and Smith, L.C. (1999). Ultraviolet irradiated spermatozoa activate oocytes but arrest preimplantation development after fertilization and nuclear transplantation in cattle. Biology of Reproduction, 61(6): 1513 - 1520.

Duarte, A.M., Diniz, E.G., Regina, M., Nascimento, B.M., Tavares, M. and Franco, R.V.R. (2007). Pregnancy rates in heifers inseminated with semen of Nellore bulls sexed by flow cytometry. Ciencia Animal Brasileira, 8: 567 - 573.

Garner, D.L. (2006). Flow cytometric sexing of mammalian sperm. Theriogenology, 65: 943 -957.

Gimes Jr, S.R. (1986). Nuclear proteins in spermatogenesis. Comparative Biochemistry Physiology Part B Comparative Biochemistry, 83: 495 – 500.

Graham, J.K., Kunze, E. and Hammerstedt, R.H. (1990). Analysis of sperm cell viability, acrosomal integrity and mitochondrial function using flow cytometry. Biology of Reproduction, 43: 55 - 64.

Maxwell, W.M. and Stojanov, T. (1996). Liquid storage of ram semen in the absence or presence of some antioxidants. Reproduction Fertility and Development, 8: 1013 – 1020.

Pena, F.J., Johannisson, A., Wallgren, M. and Rodriguez-Martinez, H. (2003). Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate. Animal Reproduction Science, 78: 85 – 98.

Smith, L.C. (1993). Membrane and intracellular effects of ultraviolet irradiation with Hoechst 33342 on bovine secondary oocytes matured in vitro. Journal of Reproduction Fertility, 99: 34 - 39.