HISTOPATHOLOGICAL EVALUATION OF ENROFLOXACIN INDUCED OXIDATIVE STRESS AND ITS AMELIORATION WITH ANTIOXIDANTS

A. Elamaran; S. Vairamuthu; S. Ramesh; P. Hariharan

doi:10.56093/ijvasr.v44i3.152934

Authors

A. Elamaran Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai – 600 007
S. Vairamuthu Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai – 600 007
S. Ramesh Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai – 600 007.
P. Hariharan Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai – 600 007.

https://doi.org/10.56093/ijvasr.v44i3.152934

Keywords:

Enrofloxacin, Alpha lipoic acid, Vitamin- E, Histopathology

Abstract

In this research work, an attempt has been made to study the histopathological changes of oxidative stress caused by enrofloxacin at therapeutic doses and its restoration by alpha-lipoic acid and its comparison with conventional antioxidant vitamin E in tissue homogenates like liver, kidney and heart of broilers. Enrofloxacin was administered to the broiler birds orally at the dose of 10 mg/kg body weight once daily from day 38 to day 42. Histopathological examination revealed marked congestion and bile duct hyperplasia of liver, degeneration and swelling of tubular epithelial cells in kidney. Interestingly, there was no appreciable lesion in the heart. Treatment with either alpha-lipoic acid (100 mg/kg body weight) or vitamin E (100 mg/kg body weight) showed moderate restoration of changes observed in liver and kidney compared to normal control birds. Co-treatment of alpha-lipoic acid (100 mg/kg body weight) and vitamin E (100 mg/kg body weight) showed restoration of liver and kidney architecture towards normal.

Downloads

Download data is not yet available.

References

Aboul-soud, M.A.M., A.M. Al-Othman, G.E. El-Desoky, Z.A. Al-Othman, K. Yusuf, J. Ahmad and A.A. Al-Khedhairy, 2011. Hepatoprotective effects of vitamin E/selenium against malathion-induced injuries on the antioxidant status and apoptosis related gene expression in rats. J. Toxicol. Sci., 36(3): 285-296.

Al-Attar, A.M., 2011. Vitamin E attenuates liver injury induced by exposure to lead, mercury, cadmium and copper in albino mice. Saudi J.

Biol. Sci., 18: 395-401.

Anadon, A., M.R. Martinez-Larranga, M.J. Diaz, P. Briugas, M.A. Martinez, M.L. Feraudez- Cruz, M.C. Fernandez and R. Fernandez, 1995.

Pharmacokinetics and residues of enrofloxacin in chickens. Am. J. Vet. Res. 56(4): 501-506.

Carreras, I., M., J.A. Castellari, G. Regueiro, L. Guerrero, E. Garcia and C. Sarraga, 2004. Influence of enrofloxacin administration and

α tocopheryl acetate supplemented diets on oxidative stability of broiler tissue. Poultry Sci., 83: 796-802.

Dulundu, E., Y. Ozel, U. Topaloglu, Ö. Sehirli, F. Ercan, N. Gedik, G. Şener, 2007. Alphalipoic acid protects against hepatic schemiareperfusion injury in rats. Pharmacology., 79: 163-170.

Niyogi, D. and M.K. Bhowmik, 2003. Toxicopathological changes of graded dose levels of ciprofloxacin. Ind. J. Anim. Sci., 73(1): 12-15.

Pamok, S., W. Aengwanich, T. Komutrin, 2009. Adaptation to oxidative stress and impact of chronic oxidative stress on immunity in heatstressed broilers. J. Thermal Biol., 34: 353–357.

Sarkar, D., P. Deb, S. Roy, T.L. Som, 1998. Toxicopathology of induced enrofloxacin in layer chicks. Ind. J. Anim. Health., 37(2): 45-48.

Srilatha, T. V., R. Ravinder , S. Qudratullah and M.V.L.N. Raju, 2010. Effect of α-lipoic acid in diet on the performance, antioxidation and

immune response in broiler chicken. Int. J. Poult. Sci., 9(7): 678-683.

Takaoka, M., Y. Kobayashi, M. Yuba, M. Ohkita and Y. Matsumura, 2001. Effects of a-lipoic acid on deoxycorticosterone acetate–salt-induced hypertension in rats. Eur. J. Pharmacol., 424: 121–129.

Packer, L. and C. Coleman, 1995. The antioxidant Miracle. New York: John Wiley & sons.