IDENTIFICATION AND ANALYSIS OF MECA GENE IN MASTITIS MILK SAMPLES COLLECTED FROM FIELD  CONDITIONS IN TAMIL NADU (INDIA)

G. Sathish; Kurunchi C. Divya; E. Hemakumar; K. Murugan; A. Stalin Vedamanickam; G. Sathiyapriya

doi:10.56093/ijvasr.v44i3.152992

Authors

G. Sathish Veterinary Assistant Surgeon, Panchetty, Animal Husbandry Department, Tiruvallur district
Kurunchi C. Divya Department of Biotechnology, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Chennai, Tamil Nadu – 603 103
E. Hemakumar Department of Biotechnology, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Chennai, Tamil Nadu – 603 103.
K. Murugan Veterinary Assistant Surgeon, Mukkarambakkam, Animal Husbandry Department, Tiruvallur district, Tamil Nadu – 601 201
A. Stalin Vedamanickam Veterinary Assistant Surgeon, Veterinary Clinician Centre, Cuddalore, Tamil Nadu - 607 001.
G. Sathiyapriya Veterinary Assistant Surgeon, Thiruvottiyur, Animal Husbandry Department, Tiruvallur district, Tamil Nadu – 600 019

https://doi.org/10.56093/ijvasr.v44i3.152992

Keywords:

mastitis milk, methicillin resistance, mecA gene, PCR, sequecing, phylogenetic analysis

Abstract

Antibiotic resistance is common problem in the treatment of bovine mastitis nowadays. Detection of methicillin resistant Staphylococcus aureus (MRSA) is community associated between environment and animals. mecA gene is major one of staphylococcal cassette chromosome mec (SCCmec) elements in acquiring methicillin resistance. Here 55 mastitis milk samples collected from Tiruvallur district were screened for Staphylococcus spp. S. aureus, S. epidermidis and S. saprophyticus were confirmed by isolation and differentiation using coagulase test and novobiocin disc sensitivity. They were further confirmed by species specific PCR. Of these 31 isolates, 5 S. aureus, 2 S. epidermidis and 1 S. saprophyticus showed positive for mecA. Phylogenetic analysis of mecA gene sequences of methicillin resistant isolates formed a group with European and US isolates. Hence further research and control is necessary for extensive spread of mecA gene in environment.

Downloads

Download data is not yet available.

References

Bardiau M, Yamazaki K, Duprez JN, Taminiau B, Mainil JG, Ote I. 2013. Genotypic and phenotypic characterization of methicillin resistant Staphylococcus aureus (MRSA) isolated from milk of bovine mastitis. Letters in Applied Microbiology, 57(3): 181-186.

Batabyal B, Kundu K R and Biswas S, 2012. Methicillin-Resistant Staphylococcus aureus: A brief review, International research journal of biological sciences, 1(7): 65-71.

Beck W D, Berger-Bachi B, Kayser F.H, 1986. Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning of mec-specific DNA. Journal of Bacteriology, 165: 373-378.

Buyukcangaz E, Kahya S, Sen A, Seyrek K, Eyigor A, Temelli S, Tayfun K. 2013. MecA Gene Prevalence in Staphylococcus aureus Isolates from Dairy Cows in Turkey. Journal of Biological and Environmental Science, 7(21): 183-190.

De Freitas M.F, Luz IDS, Silveira-Filho VDM, Júnior JWP, Stamford TL, Mota RA, LealBalbino TC. 2008. Staphylococcal toxin genes in strains isolated from cows with subclinical mastitis. Pesquisa Veterinária Brasileira, 28(12): 617-621.

Garcia-Alvarez L, Holden MT, Lindsay H, Webb CR, Brown DF, et al. 2011. Methicillin resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study. Lancet Infectious Diseases, 11: 595–603.

Jaglic Z, Michu E, Holasova M, Vlkova H, Babak V, Kolar M, Bardon J, Schlegelova J. 2010. Epidemiology and characterization of Staphylococcus epidermidis iso¬lates from humans, raw bovine milk and a dairy plant. Epidemiology and Infection 138: 772–782.

Kampf G, Lecke C, Cimbal A, Weist K, Ruden H. 1998. Evaluation of Mannitol Salt Agar for Detection of Oxacillin Resistance in Staphylococcus aureus by Disk Diffusion and Agar Screening. Journal of Clinical Microbiology 36(8): 2254–2257.

Kumar R, Yadav BR, Singh RS. 2011. Antibiotic resistance and pathogenicity factors in Staphylococcus aureus isolated from mastitic Sahiwal cattle. Journal of Biosciences, 36: 175–188.

Martineau F, Picard F, Roy P, Ouellette M, Bergeron M. 1996. Species-specific and ubiquitous DNA-based assays for rapid identification of Staphylococcus epidermidis. Journal of Clinical Microbiology, 34: 2888–2893.

Martineau F, Picard FJ, Menard C, Roy PH, Ouellette M, Bergeron MG. 2000a. Development of a rapid PCR assay specific for Staphylococcus saprophyticus and application to direct detection from urine samples. Journal of Clinical Microbiology, 38: 3280–3284.

Martineau, F., Picard, F.J., Lansac, N., Ménard, C., Roy, P.H., Ouellette, M. and Bergeron, M.G. 2000b. Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis, Antimicrobial Agents and Chemotherapy. 44: 231-238.

Pradhan P, Gopinath SM, Reddy GR, Dechamma HJ, SuryanarayanaVVS. 2011. Detection of major pathogens in bovine subclinical mastitis by multiplex PCR directly from milk samples in presence of an internal control. Indian Journal of Fundamental and Applied Life Sciences 1(4): 209- 218.

Tsubakishita S, Kuwahara-Arai K, Sasaki T and Hiramatsu K. 2011. Origin and Molecular Evolution of the Determinant of Methicillin Resistance in Staphylococci. Antimicrob Agents Chemother. 55(2): 946.