ISOLATION AND ANTIMICROBIAL SUSCEPTIBILITY OF STAPHYLOCOCCUS SCHLEIFERI SUBSP SCHLEIFERI FROM CANINE PYODERMA AND OTITIS IN CHENNAI
DOI:
https://doi.org/10.56093/ijvasr.v43i6.153658Keywords:
S.schleiferi, skin infections, dog, antibiogramAbstract
Coagulase negative Staphylococci were considered as commensal organisms or contaminants with limited pathogenic potential. However, they are becoming more frequently associated with nosocomial infections in human and skin infections in animals. In this study, we attempted to isolate and identify the Staphylococci species that causes dermatological infections in dogs. Out of 35 isolates, 27 were identified as S.pseudintermedius and 3 as S.schleiferisubspschleiferi. Among three S.schleiferi isolates, 2 were closely related to S.schleiferi ATCC strain in pta gene sequences. Methicillin resistant gene (mecA) was not detected in these isolates. All three isolates were susceptible to oxacillin, amikacin, gentamicin and tetracycline, intermediate susceptibility to ceftriaxone and resistant to Penicillin, ampicillin, sulphadiazine. S.schleiferi is an emerging cause of infections in veterinary patients. None of the isolate produced biofilm in microtitre tissue culture plate adherence method. This is the first report of isolation and identification of S.schleiferi from canine skin infections from India. Further studies are needed for early detection and virulence factors of S.schleiferi in the pathogenesis of pyoderma and otitis in dogs.
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Bannoehr J, Ben Zakour N L, Waller A S, Guardabassi L, Thoday K L, Broek A H M. and Fitzgerald J R. 2007. Population genetic structure of the Staphylococcus intermedius group: insights into agr diversification and the emergence of methicillin-resistant strains. Journal of Bacteriology, 189:8685–8692.
Bannoehr J, Franco A, Iurescia M, Battisti A and Fitzgerald JR. 2009. Molecular diagnostic identification of Staphylococcus pseudintermedius. Journal of Clinical Microbiology 47: 469–471.
Cain C L. 2013. Methicillin resistant Staphylococcal infections: Recent developments. Today’s Veterinary Practice, 3-4: 26-32
Cain CL, Morris DO, O’Shea K and Rankin SC. 2011a. Genotypic relatedness and phenotypic characterization of Staphylococcus schleiferisubspecies in clinical samples from dogs. American Journal of Veterinary Research 72:96–102.
Cain CL, Morris DO and Rankin SC. 2011b. Clinical characterization of Staphylococcus schleiferi infections and identification of risk factors for acquisition of oxacillin-resistant strains in dogs: 225 cases (2003-2009). Journal of American Veterinary Medical Association, 12:1566-1573
Frank LA, Kania SA, Hnilica KA, Wilkes, RP and Bemis, DA. 2003. Isolation of Staphylococcus schleiferi from dogs with pyoderma. Journal of American Veterinary Medical Association, 5 : 451-4.
FreneyJ, Brun Y, Bes M, Meugnier H, Grimont F, Grimont P A D, Ned C and Fleurette J.1988. Staphylococcus lugdunensissp. nov. and Staphylococcus schleiferisp. nov.,two species from human clinical specimens. International Journal of Systemic Bacteriology 38:168-172.
Hariharan H, Gibson K, Peterson R, Frankie M, Matthew V, Daniels J, Martin NA, Andrews L, Paterson T and Sharma RN. 2014. Staphylococcus pseudintermedius and Staphylococcus schleiferiSubspecies coagulans from Canine Pyoderma Cases in Grenada, West Indies, and Their Susceptibility to Beta-Lactam Drugs. Veterinary Medicine International, http://dx.doi.org/10.1155/2014/850126
Hassan A, Usman J, Kaleem F, Omair M, Khalid A and Iqbal M. 2011. Evaluation of different detection methods of biofilm formation in the clinical isolates. Brazilian Journal of Infectious Disease 15(4): 305-311
Igimi S, Takahashi E and Mitsuoka T. 1990.Staphylococcus schleiferisubsp. coagulanssubsp. nov. Isolated from the External Auditory Meatus of Dogs with External Ear Otitis. International Journal of Systemic Bacteriology, 40(4):409-411.
Yamashita K, Shimizu A, Kawano J, Uchida E, Haruna A and Igimi S. 2005. Isolation and characterization of staphylococci from external auditory meatus of dogs with or without otitis externa with special reference to Staphylococcus schleiferisubsp. coagulans isolates. Journal of Veterinary Medical Science, 67: 263.
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