EFFECT OF GLUTATHIONE ON SEMINAL QUALITY PARAMETERS AT EQUILIBRATION IN SURTI BUFFALO BULL

Kailash Kumar; Mitesh Gaur

doi:10.56093/ijvasr.v53i3.154855

Authors

Kailash Kumar Assistant Professor, Department of Veterinary Gynaecology and Obstetrics, Arawali Veterinary College, Sikar - 332 403, Rajasthan,
Mitesh Gaur Associate Professor, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur

https://doi.org/10.56093/ijvasr.v53i3.154855

Keywords:

Glutathione, Pre-freeze quality, Surti buffalo, Bubalus bubalis, Semen additive

Abstract

The main aim of this study was to investigate the effect of different concentrations of glutathione supplementation on liquid storage of Surti buffalo bull semen. This study was performed on adult Surti buffalo bull (n = 6), and seminal ejaculates (24) were collected and evaluated for various microscopic seminal quality parameters for further processing. After preliminary evaluation, ejaculates of each collection session were mixed and divided into four equal aliquots. All the aliquots were diluted (1:10) with Tris fructose egg yolk citrate extender contained glutathione served as control (T0), whereas the other three aliquots were supplemented with 0.5, 2.0 and 5.0 mM glutathione which were grouped as Treatment-1 (T1), Treatment-2 (T2) and Treatment-3 (T3), respectively. Thereafter, the samples were stored at 4 ºC for 4 h, and various seminal parameters (individual sperm progressive motility, viability, abnormalities, plasma membrane functionality) were evaluated at equilibration. The results indicated that the mean percent values for pre-freeze sperm progressive motility, live sperm percentage and HOS responsive spermatozoa were found to be significantly higher (P<0.05) (except individual progressive motility which was non-significantly higher in Treatment-3); whereas sperm abnormalities were significantly lower (P<0.05) in semen samples treated with glutathione (Treatment-1, Treatment-2 and Treatment-3) in comparison to control. The Treatment-2 (2.0 mM glutathione), had the highest pre-freeze sperm progressive motility percentage, live sperm percentage, HOS response sperm percentage and reduced sperm abnormalities percentage compared to other three groups.

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References

Andrabi, S.M.H. (2009). Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa. Reproduction in Domestic Animals, 44: 552 - 69.

Ansari, M.S., Ullah, N., Rakha, B.A. and Andrabi, S.M.H. (2011). Glutathione addition in tris-citric egg yolk extender improves the quality of cooled buffalo (Bubalus bubalis) bull semen. Pakistan Journal of Zoology, 43(1): 49 - 55.

Anzar, M., Farooq, U., Mirza, M.A., Shahab, M. and Ahmed, N. (2003). Factors affecting the efficiency of artificial insemination in cattle and buffalo in Pujab, Pakistan. Pakistan Veterinary Journal, 23: 106 - 113.

Baumber, J., Ball, B.A., Gravance, C.G., Victor, M. and Mina, C.G.D. (2000). The Effect of Reactive Oxygen Species on Equine Sperm Motility, Viability, Acrosomal Integrity, Mitochondrial Membrane Potential, and Membrane Lipid Peroxidation. Journal of Andrology, 21: 895 - 902.

Behnsawy, B.E., Shenawy, E., Siefy, E., Halawany, R.E., Sharawy, M.E., Kubota, K., Yamauchi, N. and Metwally, A.E.S. (2017). Effect of Glutathione on Cryopreservation of Buck Spermatozoa. Journal-Faculty of Agriculture Kyushu University, 62(2): 367 - 372.

Bucak, M.N. and Tekin, N. (2007). Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen. Small Ruminant Research, 73: 103 - 108.

Dushyant, Y., Vijay, S., Yadav, S.S., Patel, A., Kumar, A., Anuj, K., Sarvjeet, Y., Brijesh, K., Brijesh, Y., Pandey, R.P. and Atul, S. (2019). Effect of glutathione on viability and acrosomal integrity of bovine spermatozoa during graded cryopreservation. Journal of Pharmacognosy and Phytochemistry, 8(3): 4796 - 4800.

El-Sissy, G.A., El-Ntatat, W.R. and El-Sheshtawy, R.I. (2007). Buffalo semen quality, antioxidants and peroxidation during chilling and cryopreservation. Journal of Veterinary Research, 11: 55 - 61.

Foote, R.H., Brockett, C.C. and Kaproth, M.T. (2002). Motility and fertility of bull sperm in whole milk extender containing antioxidants. Animal Reproduction Science, 71: 13 - 23.

Funahashi, H. and Sano, T. (2005). Select antioxidants improve the function of extended boar semen stored at 10°C. Theriogenology, 63: 1605 - 1616.

Gadea, J., Selles, E., Marco, M.A., Copy, P., Matas, C., Romar, R. and Ruiz, S. (2004). Decrease in glutathione content in boar sperm cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology, 62: 690 - 701.

Gangwar C., Saxena, A., Patel, A., Singh, S.P., Yadav, S., Kumar, R. and Singh, V. (2018). Effect of reduced glutathione supplementation on cryopreservation induced sperm cryoinjuries in Murrah bull semen. Animal Reproduction Science, 192: 171 - 178.

Ismail, L.K. and Darwish, S.A. (2011). Effect of glutathione (GSH) on microscopic parameters and DNA integrity in Egyptian buffalo semen during liquid and frozen storage. Journal of Reproduction Infertility, 2(3): 32 - 40.

Kadirvel, G., Satish, K. and Kumaresan, A. (2009). Lipid peroxidation, mitochondrial membrane potential and DNA integrity of spermatozoa in relation to intracellular reactive oxygen species in liquid and frozen-thawed buffalo semen. Animal Reproduction Science, 114: 125 - 134.

Mittal, J.P. and Pandey, M.D. (1972). Evaluation of semen quality of Barberi and Jamnapari bucks. Indian Journal Animal Production, 2: 14 - 19.

Munsi, M.N., Bhuiyan, M.M.U., Majumder, S. and Alam, M.G.S. (2007). Effects of exogenous glutathione on the quality of chilled bull semen. Reproduction in Domestic Animal, 42: 358 - 362.

Nichi, M., Bols, P.E.J., Zuge, R.M., Barnabe, V.H., Goovaerts, I.G.F., Barnabe, R.C. and Cortada, C.N.M. (2006). Seasonal variation in semen quality in Bos indicus and Bos taurus bulls raised under tropical conditions. Theriogenology, 66: 822 - 828.

Pandey, R.P., Sinhe, S.N., Singh, B. and Akhtar, M.H. (1985). Characteristic of semen and fertility rate in Saanen and Barbari bucks. Indian Journal of Animal Science, 55: 773-774.

Salisbury, G.W., Van Denmark, N.L. and Lodge, J.R. (1978). Physiology of reproduction and artificial insemination of cattle. Freeman, San Francisco. 494 - 554.

Sansone, G., Nastri, M.J.F. and Fabbrocini, A. (2000). Storage of buffalo (Bubalus bubalis) semen. Animal Reproduction Science, 62: 55 - 76.

Sendecor, G.W. and Cochran, W.G. (1994). Statistical methods, 8th Edition, Iowa State University Press, Ames.

Shamsuddin, M., Amiri, Y. and Bhuiyan, M.M.U. (2000). Characteristics of buck semen with regards to ejaculate numbers, collection intervals, dilution and presentation periods. Reproduction in Domestic Animal, 35: 53 - 57.

Sinha, M.P., Singha, A.K. and Prasad, R.L. (1996). The effect of glutathione on the motility, enzyme leakage and fertility of frozen goat semen. Animal Reproduction Science, 41: 237 - 243.

Slaweta, R. and Laskowska, T. (1987). The effect of glutatione on the motility and fertility of frozen bull semen. Animal Reproduction Science, 13: 249 - 253.

Sreejith, J.N., Brar, A.S., Ahuja, C.S., Sangha, S.P.S. and Chaudhary, K.C. (2006). A comparative study on lipid peroxidation, activities of antioxidant enzymes and viability of cattle and buffalo bull spermatozoa during storage at refrigeration temperature. Animal Reproduction Science, 96: 21 - 29.

Stradaioli, G., Noro, T., Sylla, L. and Monaci, M. (2007). Decrease in glutathione (GSH) content in bovine sperm after cryo-preservation: comparison between two extenders. Theriogenology, 67: 1249 - 1255.

Vishwanath, R., and Shannon, P. (1997). Do sperm cells age? A review of the physiological changes in sperm during storage at ambient temperature. Reproduction, Fertility and Development, 9: 321 - 332.

Vishwanath, R., Pitt, C.P. and Shannon, P. (1996). Sperm numbers, semen age and fertility in fresh and frozen bovine semen. Proceedings of the New Zealand Society of Animal Production, 56: 31 - 34.

Watson, P.F. (2000). The cause of reduced fertility with cryopreserved semen. Animal Reproduction Science, 60(1): 481- 492.

Yeste, M., Flores, E., Estrada, E., Bonet, S., Rigau, T. and Rodrıguez-Gil, J.E. (2012). Reduced glutathione and procaine hydrochloride protect the nucleoprotein structure of boar spermatozoa during freeze-thawing by stabilizing disulfide bonds. Reproduction, Fertility and Development, 25(7): 1036- 1050.