EVALUATION OF NON STARCH POLYSACCHARIDE DEGRADING ENZYMES AND ENZYME COMBINATIONS FOR THEIR ABILITY TO DEGRADE NON STARCH POLYSACCHARIDES OF LAYER DIET BY TWO STAGE IN VITRO DIGESTION ASSAY

Abstract

The investigation was undertaken to evolve a suitable NSP enzyme combination with potential to improve the digestibility of corn and soybean  meal based diets for layers. Two stage in vitro NSP digestibility assay was carried to arrive at a suitable NSP enzyme combination comprising of xylanase, cellulase and â-D-glucanase for corn-soybean meal based layer  diets. The degree of NSP digestibility was assayed by measuring the amount of total sugars released. Various concentrations of Non-starch Polysaccharide
 enzymes viz., xylanase (0-60000 IU/kg), cellulase (0-6400 IU/kg) and â-D glucanase (0-6400 IU/kg) were screened by in vitro digestibility assay. From that three best concentrations of NSP enzymes were selected for each enzyme to formulate twenty seven (3x3x3) enzyme combinations.  The in vitro sugars release for layer diet was significantly higher (P<0.01) for concentrations of 5000, 7500 and 10000 xylanase IU/kg. Where as cellulase concentrations of 400, 800 and 1600 and â-D-glucanase concentrations of 50, 100 and 3200 IU/kg diet resulted in higher (P<0.01) sugar release. Among the twenty seven enzyme combinations of xylanase, cellulase and â-D-glucanase, the combination comprising of xylanase-10000, cellulase-400 and â-D-glucanase 100 IU/kg was selected for layer diet. It can be concluded that In vitro NSP digestibility assay, simulating the gut conditions of poultry could be used as a simple and effective technique to evolve suitable NSP enzyme combination with most effective concentration of enzymes specifically for diets of layers formulated with various feed ingredients and also to arrive at best effective dosage level.