ISOLATION,  IDENTIFICATION  AND  ANTIBIOGRAM  PATTERN OF AVIBACTERIUM  PARAGALLINARUM  FROM  JAPANESE  QUAILS

V. Thenmozhi; S. Malmarugan

doi:10.56093/ijvasr.v9i4.155782

Authors

V. Thenmozhi Department of Veterinary Microbiology, Veterinary College and Research Institute, Namakkal- 637 002
S. Malmarugan Department of Veterinary Microbiology, Veterinary College and Research Institute, Namakkal- 637 002

https://doi.org/10.56093/ijvasr.v9i4.155782

Keywords:

Japanese quail, Avibacterium paragallinarum, Polymerase Chain Reaction, Antibiogram

Abstract

A total of 53 trachea, air sacs, lung, and infra orbital sinus exudates were collected from 5 commercially reared Japanese quail farms showing typical symptoms of infectious coryza. Avibacterium paragallinarum was isolated and identified using selective media and by biochemical identification methods. Biochemical identification tests such as satellitism, IMViC tests and carbohydrate fermentation tests were performed. In total, eight A. paragallinarum isolates were isolated and identified from the field samples and the species were confirmed with the species specific PCR, which gives the amplicon size of 500bp. Antibiogram of A. paragallinarum showed 100% resistance for Ampicillin, neomycin, Pefloxacin, co-trimaxazole, furazolidone, streptomycin, Cephalexin and Amikacin with 90% for Gentamycin and 70% for oxytetracycline.

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