PATHOGENICITY  STUDY  OF  FIELD  ISOLATES  OF  AVIBACTERIUM PARAGALLINARUM  IN  SEVEN  WEEK  OLD  CHICKS.

Durairajan Ramachandran; Vadivazhagan Kalimuthu; Mandeep Sharma

doi:10.56093/ijvasr.v9i4.155785

Authors

Durairajan Ramachandran Department of Veterinary Microbiology and Immunology, G. C. Negi College of Veterinary and Animal Sciences, CSKHPKV, Palamapur, H.P-176 062
Vadivazhagan Kalimuthu Department of Veterinary Microbiology and Immunology, G. C. Negi College of Veterinary and Animal Sciences, CSKHPKV, Palamapur, H.P-176 062
Mandeep Sharma Department of Veterinary Microbiology and Immunology, G. C. Negi College of Veterinary and Animal Sciences, CSKHPKV, Palamapur, H.P-176 062

https://doi.org/10.56093/ijvasr.v9i4.155785

Keywords:

Virulence, Avibacterium paragallinarum

Abstract

Avibacterium paragallinarum isolated from various places in northern India were studied for their virulence by infecting the experimental chicks. An inoculum of 0.2 ml (1011 cfu/ml) was dropped in the nostrils of birds or injected in the right infraorbital sinus of three seven-week-old chickens for each isolate. Birds infected with A. paragallinarum showed typical signs of Infectious Coryza. Bacteria could be reisolated from the birds. The identified bacteria and the direct clinical samples subjected to species specific HP2PCR for A. paragallinarum. PCR was found to be specific and it produced 500bp amplicons. In the challenge study on the experimental birds, accumulation of whitish fluid like exudates in the infraorbital sinuses was more prominent and air sacculitis and pneumonia noticed less frequently in birds.

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