SCREENING OF  STAPHYLOCOCCUS  AUREUS  ISOLATES  FROM MASTITIS  FOR  ANTIBACTERIAL  SUSCEPTIBILITY  PATTERN  AND BETALACTAMASE  PRODUCTION

S.Pavulraj; S.Amsaveni; L. Kalaiselvi; S.Ramesh

doi:10.56093/ijvasr.v9i4.156520

Authors

S.Pavulraj Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai-600 007
S.Amsaveni Department of Veterinary Pharmacology and Toxicology Madras Veterinary College, Chennai-600 007
L. Kalaiselvi Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai-600 007
S.Ramesh Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai-600 007

https://doi.org/10.56093/ijvasr.v9i4.156520

Keywords:

Staphylococcus aureus, ß-lactamase, Antimicrobial resistance, Mastitis, resazurin dye test

Abstract

Isolates of Staphylococcus aureus from bovine clinical and subclinical mastitis were tested for ß-lactamase production and antimicrobial susceptibility pattern. Beta-lactamase production was assessed by penicillin-phenol red acidometric test. Antimicrobial susceptibility was tested by measuring MIC by Resazurin dye broth microdilution method. Forty per cent of isolates tested were positive for â-lactamase production. The susceptibility of S. aureus isolates were: gentamicin (100%), enrofloxacin (70%), rifampin (70%) and tetracycline (40%). Complete resistance was observed for amoxycillin, ceftriaxone, nalidixic acid, erythromycin, sulphamethoxazole and trimethoprim. The results of the study indicate the widespread resistance of Staphylococcus aureus isolates against commonly used antimicrobials and also the utility of resazurin test and ß-lactamase test to study antimicrobial drug resistance.

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