EFFECT OF DOMINANT FOLLICLE ABLATION ON OVARIAN FOLLICLE COUNT IN SAHIWAL CATTLE
190 / 86
Keywords:
Dominant follicle ablation, Follicular count, FSH, Ovum pick-upAbstract
The present study was conducted to investigate the effect of dominant follicle ablation on follicle count in Sahiwal cattle. All the selected donor animals were randomly divided into two equal groups. Donor cows in group 1(n=10) were subjected to dominant follicle(s) ablation, donor cows in group 2(n=10) without ablation and both groups were administered with 10 µg GnRH followed by FSH in 3 divided doses @ 100 mg, 60 mg and 40 mg at 48, 60 and 72 hrs after GnRH administration. OPU was carried out 24 hrs after the last FSH administration in all the donor cows. The overall mean number of follicles available for aspiration by Ovum pick up (OPU) method were 7.43 ± 0.22 in group 1 and 6.19 ± 0.22 in group 2 and the difference in the number of follicles between groups was significant (p<0.05). Mean follicular count on right and left ovaries of group 1 cows were 7.87 ± 0.30 and 7.00 ± 0.30, respectively with a significant difference (p>0.05). whereas, mean follicular count on right and left ovaries of group 2 cows were 6.14 ± 0.32 and 6.24 ± 0.32, respectively without significant difference (p<0.05). While, the mean follicular count in group 1 at different timings of 0 hr, 48 hrs and 96 hrs were 7.15 ± 0.36, 7.60 ± 0.36 and 7.55 ± 0.36, respectively and in group 2 cows were 6.03 ± 0.39, 6.25 ±0.39 and 6.30 ± 0.39, respectively without significant (p>0.05). In conclusion, ablation of dominant follicle had higher follicular count in Sahiwal donor cows compared to non- ablation group cows.
Downloads
References
REFERENCES
Amiridis, G. S., Tsiligianni, T. and Vainas, E. (2006). Follicle ablation improves the ovarian response and the number of collected embryos in superovulated cows during the early stages of lactation. Reproduction in domestic animals; 41(5): 402-407.
Baracaldo, M. I., Martinez, M. F., Adams, G. P. and Mapletoft, R. J. (2000). Superovulatory response following transvaginal follicle ablation in cattle. Theriogenology; 53(6): 1239-1250.
Bergfelt, D. R., Lightfoot, K. C. and Adams, G. P. (1994). Ovarian synchronization following ultrasound-guided transvaginal follicle ablation in heifers. Theriogenology; 42(6): 895-907.
Bergfelt, D. R., Bo, G. A., Mapletoft, R. J. and Adams, G. P. (1997). Superovulatory response following ablation-induced follicular wave emergence at random stages of the oestrous cycle in cattle. Animal Reproduction Science; 49(1): 1-12.
Callesen, H., Greve, T. and Christensen, F. (1987). Ultrasonically guided aspiration of bovine follicular oocytes. Theriogenology; 27(1): 217.
Chaubal, S. A., Molina, J. A., Ohlrichs, C. L., Ferre, L. B., Faber, D. C., Bols, P. E. J. and Yang, X. (2006). Comparison of different transvaginal ovum pick up protocols to optimise oocyte retrieval and embryo production over a 10-week period in cows. Theriogenology; 65(8): 1631-1648.
Ciftci, M. F. and Dinc, D. A. (2023). The effect of different FSH administration before ovum pick up on superstimulation response and oocyte yield. Reproduction in Domestic Animals; 58(8): 1055-1062
Getz, I., Karadjole, M., Samardzija, M., Makek, Z., Matkovic, M., Dobranic, T. and Cergolj, M. (2007). Comparison of different synchronization protocols on OPU/ IVF results in FSH stimulated Simmental heifers. Veterinarski arhiv ; 81(5) : 545-556.
Grasso, F., Guilbault, L. A., Roy, G. L. and Lussier, J. G. (1989). Ultrasonographic determination of ovarian follicular development in superovulated heifers pretreated with FSH-P at the beginning of the estrous cycle. Theriogenology; 31(6): 1209-1220.
Kim, I. H., Son, D. S., Yeon, S. H., Choi, S. H., Park, S. B., Ryu, I. S. and Yoon, J. T. (2001). Effect of dominant follicle removal before superstimulation of follicular growth, ovulation and embryo production in Holstein cows. Theriogenology; 55(4): 937-945.
Matoba, S., Yoshioka, H., Matsuda, H., Sugimura, S., Aikawa, Y., Ohtake, M. and Imai, K. (2014). Optimizing production of in vivo-matured oocytes from superstimulated Holstein cows for in vitro production of embryos using X-sorted sperm. Journal of Dairy Science; 97(2): 743- 753.
Nawaz, M., Saleem, M., Ullah, F., Shabbir Khan, G., Zahoor, I., Ahmad, N. and Riaz, A. (2022). Exogenous progesterone-dependent modulation in the follicular dynamics of Bos indicus cattle undergoing repeated ovum pick-up sessions. Reproduction in Domestic Animals; 57(1): 55-63.
Ongaratto, F. L., Rodriguez-Villamil, P., Tribulo, A. and Bó, G. A. (2018). Effect of follicle wave synchronization and gonadotropin treatments on the number and quality of cumulus-oocyte complex obtained by ultrasound-guided ovum pick-up in beef cattle. Animal Reproduction (AR); 12(4): 876-883.
Shaw, D.W. and Good, T. E. (2000). Recovery rates and embryo quality following dominant follicle ablation in superovulated cattle. Theriogenology; 53: 1521- 1528.
Srimannarayana. (2019). Optimization of oocyte retrieval through trans vaginal ovum pickup in Ongole (Bos indicus) cows. M.V.Sc Thesis submitted to Sri Venkateshwara Veterinary University, Tirupati, AP.
Snedecor, G. W. and Cochran, W. G. (1994). Statistical methods, 8th Edition. lowa.
Viana, J. (2019). Statistics of embryo production and transfer in domestic farm animals. Embryo Technology Newsletter; 36(4): 17.
Webb, R., Campbell, B. K., Garverick, H. A., Gong, J. G., Gutierrez, C. G. and Armstrong, D. G. (1999). Molecular mechanisms regulating follicular recruitment and selection. Journal of reproduction and fertility Supplement; 54:33-48.
Downloads
Submitted
Published
Issue
Section
License
All the copy right belongs to the sponsoring Organization, Tamil Nadu Veterinary and Animal Sciences University, Chennai - 51.