BIOCHEMICAL AND ANTIOXIDANT PROFILES IN AFLATOXIN B1 INDUCED LIVER DAMAGE AND ITS AMELIORATION BY USING AGED GARLIC EXTRACT IN WISTAR ALBINO RATS

K. Sarvesha; M.L. Satyanarayana; S. Rao; S. Yathiraj; N.B. Shridhar

doi:10.56093/ijvasr.v44i5.173547

Authors

K. Sarvesha Ph.D. SCholar, Department of Pathology, Veterinary College, Hebbal, KVAFSU, Bengaluru - 560 024
M.L. Satyanarayana Professor, Department of Pathology, Veterinary College, Hebbal, KVAFSU, Bengaluru - 560 024
S. Rao Professor, Department of Pathology, Veterinary College, Hebbal, KVAFSU, Bengaluru - 560 024
S. Yathiraj Dean, Veterinary College, Hebbal, KVAFSU, Bengaluru - 560 024
N.B. Shridhar Associate Professor, Department of Pharmacology and Toxicology, Veterinary College, Hebbal, KVAFSU, Bengaluru - 560 024

https://doi.org/10.56093/ijvasr.v44i5.173547

Keywords:

Aged garlic extract (AGE), Aflatoxin B1 (AFB1), Antioxidants

Abstract

The present study was conducted to evaluate hepatoprotective effect of aged garlic extract in aflatoxin B1 induced liver damage in Wistar albino rats. In this study the liver damage was induced by inoculating aflatoxin B1 intraperitoneally at the dose rate of 4 mg/kg body weight on day 12 of the experimental study. Aged garlic extract was used at the dose rate of 200 mg/kg body weight orally through orogastric tube for a period of 28 days against aflatoxin B1 induced liver injury. In aflatoxin B1 treated rats there was significant increase in liver enzymes and significant decrease in the enzymic antioxidant profile was observed, but these liver enzymes and antioxidant levels were with in the normal range in aged garlic extract and aflatoxin B1 treatment rats, indicating hepatoprotective effect of aged garlic extract against aflatoxin B1 induced liver damage.

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References

Adel, A., Tarek, M.K. and Nermin, A.H. (2008). Effect of some natural antioxidants on aflatoxin B1 induced hepatic toxicity. J. EXCLI., 7:119-131.

Biren, S.N., Nayak, B.S., Patel, M.A., Mishra, A.D. and Avinash, S. (2006). Aged garlic extract– A health benefit review. Pharmacognosy

Magazine, 2(6):90-95.

Bruce, A.J. and Baudry, M. (1995). Oxygen free radicals in rat limbic structures after kainate- induced seizures. Free Radic Biol Med., 18: 993–1002.

Claiborne, A. (1985). Catalase activity. In: Greenwald RA (ed) CRC Handbook of Methods for Oxygen Radical Research. CRC Press, Boca Raton, pp 283-284.

Elsaid, F.G. (2006). Aqueous Garlic Extract and Sodium Thiosulphate as Antidotes for Cyanide Intoxication in Albino Rats. R e s . J. Medicine and Med. Sci., 1(2): 50-56.

Farombi, E.O., Adepoju, B.F., Ola-davies, O.E. and Emerole, G.O. (2005). Chemoprevention of aflatoxin B1-induced genotoxicity and hepatic oxidative damage in rats by kolaviron, a natural bioflavonoid of Garcinia kola seeds. Eur. J. Cancer Prev., 14(3):207-14.

Hong, L. I. and Yan. (2002). Effects of bicyclol on aflatoxin B1 metabolism and hepatotoxicity in rats. Acta Pharmacol. Sin., 23 (10): 942-945.

Marklund, S. and Marklund, G. (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem., 47:469–74.

Roebuck, B.D. and Maxuitenko, Y.Y. (1994). Biochemical mechanisms and biological implications of the toxicity of aflatoxins as related to aflatoxin carcinogenesis. In The Toxicol. of Aflatoxins (D. L. Eaton and J. D. Groopman, Eds.), pp. 27–43. Academic Press, San Diego.

Rotruck, J.T., Pope, A.L., Ganther, H.E., Swanson, A.B., Hafeman, D.G. and Hoekstra, W.G. (1973). Selenium: biochemical role as a component of glutathione peroxidase. Sci., 179:588-90.

Sharmila, B.G. and Murugesan. (2009). Ethanolic leaves extract of Trianthema Portulacastrum L. ameliorates Aflatoxin B1 induced hepatic damage in rats. Ind. J. Clin. Biochem., 24 (3):250-256.

Sivanesan, D. and Begum, H.V. (2007). Preventive role of Gynandropsis gynandra L., against aflatoxin B1 induced lipid peroxidation and antioxidant defense mechanism in rats. Ind. J. Exp. Biol., 45:299-303.

Tadi, P.P., Teel, R.W. and Lau, B.H.S. (1991). Organosulfur compounds of garlic modulate mutagenesis, metabolism and DNA binding of aflatoxin B1. Nutr. Cancer., 15: 87–95.

Wood, G. E. (1989). Aflatoxins in domestic and imported foods and feeds. J. Assoc. Off. Anal. Chem. 72: 543–548.

Yamasaki, T. and Lau, B.H.S. (1997). Garlic compounds protect vascular endothelial cells from oxidant injury. Folia Pharmacol. Jpn.,

110(1):138–141.