ISOLATION AND IDENTIFICATION OF ESCHERICHIA COLI FROM BROILERS AND LAYERS IN NAMAKKAL

S. Deepadharshini; G. Selvaraju; S. Saravanan; K. Sukumar

doi:10.56093/ijvasr.v55i1.175146

Authors

S. Deepadharshini M.V.Sc. Scholar, Department of Veterinary Public Health and Epidemiology, Veterinary Colllege and Research Institute, TANUVAS, Namakkal - 637 002
G. Selvaraju Professor and Head, Department of Veterinary Public Health and Epidemiology, Veterinary College and Research Institute, TANUVAS, Namakkal - 637 002
S. Saravanan Professor, Department of Veterinary Public Health and Epidemiology, Veterinary College and Research Institute, TANUVAS, Namakkal - 637 002
K. Sukumar Professor and Head, Department of Veterinary Microbiology, Veterinary College and Research Institute, TANUVAS, Namakkal - 637 002

https://doi.org/10.56093/ijvasr.v55i1.175146

Keywords:

Broiler, layers, Escherichia coli, molecular identification

Abstract

A study was undertaken to isolate, identify and determine the prevalence of Escherichia coli in broilers and layers in Namakkal, a major poultry production hub in South India. A total of 200 samples were collected aseptically, consisting of 100 broiler meat samples obtained from retail outlets and 100 cloacal swabs collected from commercial layer farms. Isolation was carried out using standard cultural procedures, followed bybiochemical characterization to confirm presumptive E. coli. Molecular identification was performed by targeting the 16S rRNA gene. All 129 isolates that were presumptively identified as E. coli yielded the expected 410 bp amplicon, validating the accuracy of phenotypic identification. The prevalence of E. coli was 66.00 per cent in broiler meat samples, indicating considerable contamination at the retail level and emphasizing the need for improved hygienic handling and processing practices. In layer birds, 63.00 per centpositivity in cloacal swabs reflected persistent intestinal carriage and the potential for environmental dissemination. Overall, the findings demonstrate a high occurrence of E. coli across different stages of the poultry chain and underline the importance of continuous microbiological surveillance to support food safety, guided intervention strategies and reduced public health risks.

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References

Arulkumar, T., Subramanian, S. and Sasikala, M. (2014). Assessment of food safety with reference to Escherichia coli in broiler chicken meat. Journal of Pure and Applied Microbiology, 8(4): 3335–3337.

Balasubramaniam, A., Eswaran, M.A, Suresh, P. and Sukumar, K. (2014). Detection of tetracycline resistance determinant tetA gene and antimicrobial resistance pattern in Escherichia coli isolates recovered from healthy layer chickens. Veterinary World, 7(9): 635–638.

Barnes, H.J. and Gross, W.B. (1997). Colibacillosis. In: B.W. Calnek (Ed.), Diseases of Poultry (10th ed., pp. 131–139). Mosby-Wolfe Publication Ltd., London, UK.

Board, R.G. (1968). Microbiology of the egg: A review. In: T.C. Carter (Ed.), Egg Quality: A Study of the Hen’s Egg (British Egg Marketing Board Symposium No. 4, pp. 133–162). Oliver & Boyd, Edinburgh.

Cruickshank, R., Duguid, J.P. and Swain, R.H.A. (1972). Medical Microbiology (11th ed.). E. & S. Livingstone Ltd., UK, pp. 222–223.

Mandal, A.K., Talukder, S., Hasan, M.M., Tasmim, S.T., Parvin, M.S., Ali, M.Y. and Islam, M.T. (2022). Epidemiology and antimicrobial resistance of Escherichia coli in broiler chickens, farmworkers and farm sewage in Bangladesh. Veterinary Medicine and Science, 8(1): 187–199.

Marangoni, F., Corsello, G., Cricelli, C., Ferrara, N., Ghiselli, A., Lucchin, L. and Poli, A. (2015). Role of poultry meat in a balanced diet aimed at maintaining health and wellbeing: an Italian consensus document. Food and Nutrition Research, 59: 27606.

Panchal, P.P., Patel, J.M., Vihol, P.D., Raval, J.K., Patel, D.R., Danger, N., Kalyani, I.H. and Patel, N.M. (2020). Serotyping and prevalence of Escherichia coli infection in poultry of South Gujarat based on culture isolation and identification. International Journal of Current Microbiology and Applied Sciences, 9(10): 3876–3883.

Rehault-Godbert, S., Guyot, N. and Nys, Y. (2019). The golden egg: nutritional value, bioactivities and emerging benefits for human health. Nutrients, 11(3): 684.

Sannat, C., Patyal, A., Rawat, N., Ghosh, R.C., Jolhe, D.K., Shende, R.K., Hirpurkar, S.D. and Shakya, S. (2017). Characterization of Salmonella gallinarum from an outbreak in Raigarh, Chhattisgarh. Veterinary World, 10(2): 160–165.

Tegegne, H., Filie, K., Tolosa, T., Debelo, M. and Ejigu, E. (2024). Isolation and identification of Escherichia coli O157:H7 recovered from chicken meat at Addis Ababa slaughterhouses. Infection and Drug Resistance, 17: 851–863.

Vasanthi, B., Parthiban, M., Sarathchandra, G., Narendra Babu, N. and Brindha, K. (2023). Molecular characterization and antimicrobial resistance profiling of food-borne pathogens from chicken meat in Tamil Nadu, India. The Indian Veterinary Journal, 100(10): 35–38.