EFFECT OF ORGANIC ACID SUPPLEMENTATION IN DRINKING WATER ON HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF JAPANESE QUAIL

Farqad Shawqi Ibrahim; Ali H Al-obaidy

doi:10.56093/ijvasr.v55i3.179281

Authors

Farqad Shawqi Ibrahim Department of Animal Production, College of Agricultural, University of Diyala, Iraq. MJJ2+R9G
Ali H Al-obaidy Department of Animal Production, College of Agricultural, University of Diyala, Iraq. MJJ2+R9G

https://doi.org/10.56093/ijvasr.v55i3.179281

Keywords:

Acetic acid, blood biochemistry, citric acid, haematology parameters, Japanese quail

Abstract

This study aimed to evaluate the effects of supplementation of a mixture of acetic acid and citric acid in drinking water on hematological and biochemical parameters in male and female Japanese quails. A total of 540 day-old quails were randomly distributed into four treatments and three replicates with 135 birds for each group as follows: T1 drinking water without acids; T2, T3, and T4 treatments with 1ml+1g, 2ml+2g, and 3ml+3g of acetic acid + citric acid per liter of drinking water, respectively. At 42 days of age, blood samples were collected from six quails per treatments (three repicats per sex)for haematological and biochemical analysis. The results showed that the organic acid mixture significantly (P≤0.01) increased red blood cell count, packed cell volume, haemoglobin concentration, and high-density lipoprotein levels in both males and females. It also significantly decreased cholesterol, triglyceride, low-density lipoprotein, and aspartate aminotransferase levels in both sexes. Significant increase in total protein(P≤0.05) and globulin (P≤0.01) levels in males, while albumin levels increased significantly (P≤0.01) in females was also observed. In conclusion, adding acetic and citric acids to drinking water positively influenced haematological and biochemical parameters, indicating enhanced health and productive performance in Japanese quails.

Downloads

Download data is not yet available.

References

Abd-Elsamee, M.O., Motawe, H.F.A., Selim, M.M. and Elsherif, H.M.R. (2020). Effect of different dietary crude protein levels and citric acid on broiler chickens’ performance, carcass characteristics, intestinal morphology, and blood components. World’s Veterinary Journal, 3: 362-374. https://doi.org/10.36380/scil.2020. wvj45

Abou–Ashour, A.M.H., El-Naga, A., Manal, K., Hussein, E.A. and El- Bana, Z. (2021). Effect of dietary citric, acetic acids or their mixture on broiler chicks performance, carcass characteristics and some intestinal histomorphological parameters. Egyptian Journal of Nutrition and Feeds, 24(1): 119-138. https://doi. org/10.21608/ejnf.2021.170317

Alaeldein, M., Abdullah, H., Yousif, M. and Rifat, U. (2017). Effect of organic acid and Bacillus subtilis alone or together on growth traits, biochemical and antioxidant status in broilers exposed to Salmonella. typhimurium challenge during starter phase. Journal of Applied Animal Research, 45(1): 41-50. http://dx.doi.org/10.1080/09712119.2016.1219665

Al-Fadhli, M.K.M., Abbas, N.Q. and Jalil, Q.A.M.J. (2018). Effect of formic acid supplementation on some serum biochemical parameters of broiler chickens. Arab Journal for Sciences and Research Publishing, 3(2): 42-51. https://doi.org/10.26389/AJSRP. M080518

Al-Tememy, A.T.D., Al-obaidy, A.H. and Wasman, P.H. (2023). Adding sodium citrate in water and effect in physiological performance of broiler chickens reared under high-density condition. IOP Conference Series: Earth and Environmental Science. 1252(1): 1-7. https://doi.org/10.1088/1755-1315/1252/1/012151

Archer, R.K. (1965). Haematological techniques for use on animals. Blackwell Scientific publications, London.

Coles, E.H. (1986). Veterinary clinical Pathology. 4th ed. W. B. saunders. Philadelphia, London, Hong kong.

Dehghani-Tafti, N. and Jahanian, R. (2016). Effect of supplemental organic acids on performance, carcass characteristics, and serum biochemical metabolites in broilers fed diets containing different crude protein levels. Animal Feed Science and Technology, 211: 109-116. https://doi.org/10.1016/j. anifeedsci.2015.09.019

Deng, Q., Shi, H., Luo, Y., Liu, N. and Deng, X. (2020). Dietary lactic acid bacteria modulate yolk components and cholesterol metabolism by HMGR pathway in laying hens. Brazilian Journal of Poultry Science, 22:1-8. https://doi.org/10.1590/1806-9061-2020-1261

Du, H., Sarwar, I., Ahmad, S., Suheryani, I., Anjum, S., Andlib, S. and Arain, M. A. (2024). Organic acids in poultry industry: a review of nutritional advancements and health benefits. World’s Poultry Science Journal, 80(1):133-153. https://doi.org/10.1080/00439339.2023.2262435

Duncan, D.B. (1955). Multiple aange and multiple F-tests. Biometrics, 11: 1-42. http://dx.doi.org/10.2307/3001478

Elnagar, A. and Abo EL-Maaty H.M.A. (2017). Impact of using organic acids on growth performance, blood biochemical and hematological traits and immune response of ducks (Cairina moschata). Egyptian Poultry Science Journal, 37(3): 907-925. https://doi.org/10.21608/ EPSJ.2017.7740

Fikry, A.M., Attia, A.I., Ismail, I.E., Alagawany, M. and Reda, F.M. (2021). Dietary citric acid enhances growth performance, nutrient digestibility, intestinal microbiota, antioxidant status, and immunity of Japanese quails. Poultry Science, 100(9): 101326. https://doi.org/10.1016/j. psj.2021.101326

Fouladi, P., Ebrahimnezhad, Y., Shahryar, H.A., Maheri, N. and Ahmadzadeh, A. (2018). Effects of organic acids supplement on performance, egg traits, blood serum biochemical parameters and gut microflora in female Japanese quail (Coturnix coturnix japonica). Brazilian Journal of Poultry Science, 20: 133-144. https://doi.org/10.1590/1806-9061-2016-0375

Franey, R.J., and Elias A. (1968). Serum cholesterol measurement based on ethanol extraction and ferric chloride- sulfuric acid. Clinica Chimica Acta, 21:255-263. https://doi. org/10.1016/0009-8981(68)90135-6

Gayirbegov, D.S., Ibrahim, I.F.S., Andreev, A.I., Gibalkina, N.I. and Kostromkina, N.V. (2018). Influence of the m-feed additive on metabolism and productivity of laying quails. OnLine Journal of Biological Sciences, 18(2): 221-225. https://doi.org/10.3844/ojbsci.2018.221.225

Ghazvinian, K., Seidavi, A., Laudadio, V., Ragni, M. and Tufarelli, V. (2018). Effects of various levels of organic acids and of virginiamycin on performance, blood parameters, immunoglobulins and microbial population of broiler chicks. South African Journal of Animal Science, 48(5): 961-967. https://doi. org/10.4314/sajas.v48i5.16

Henry, R.J., Sobel, C. and Kim, J. (1957). A modified carbonate-phosphotungstate method for the determination of uric acid and comparison with the spectrophotometric uricase method. American Journal of Clinical Pathology, 28(2): 152-160. https:// doi.org / 10.1093/ ajcp/28.2.152

Jasim, M.S. and Taha, A.Y. (2017). Use of supplementation of probiotic with different levels of citric acid as prometer for productive performance of laying hens. Diyala Agricultural Sciences Journal, 9(special Issue):1–15. Retrieved from https://journal. djas.uodiyala.edu.iq /index.php/dasj/ article/view/3524

Kaya, A., Kaya, H., Gül, M., Apaydin Yıldırım, B. and Tımurkaan, S. (2015). Effect of different levels of organic acids in the diets of hens on laying performance, egg quality criteria, blood parameters, and intestinal histomorphology. Indian Journal of Animal Research, 49(5): 645-651. https://doi.org/10.18805/ijar.5577

Khan, S.H. and Iqbal, J. (2016). Recent advances in the role of organic acids in poultry nutrition. Journal of Applied Animal Research, 44(1): 359-369. https://doi.org/10.1080/09712119.2015.1079527.

Ma, J., Mahfuz, S., Wang, J. and Piao, X. (2021). Effect of dietary supplementation with mixed organic acids on immune function, antioxidative characteristics, digestive enzymes activity, and intestinal health in broiler chickens. Frontiers in nutrition, 8: 1-15. https://doi. org/10.3389/fnut.2021.673316

Makofane, V., Ng’ambi, J.W. and Gunya, B. (2022). The effect of citric acid supplementation on growth performance, digestibility and linear body measurement of ross 308 broiler chickens: a review. Indian Journal of Animal Research, 56(4): 387-391. https://doi.org/10.18805/IJAR.BF-1433

Natt, M. and Herrick, C. (1952). A new blood diluents for counting the erythrocytes and leukocytes of the chicken. Poultry Science. 31: 735-738.

Ndelekwute, E.K., Uzegbu, H.O., Madu, H.C. and Assam, E.M. (2012). Organic acids as feed additive in pig and poultry diets: a review. Nigeria Agricultural Journal, 43(1): 29-39 https://www.ajol.info/index.php/naj/ article/view/110100

Orbugh, A.T., Adeyeye, E.A., Lala, A.O., Durojaye, O.J., Ogunleye, J.B., Sogunle, O.M. and Oso, A.O. (2023). Effect of organic acid blend on the growth performance, apparent nutrient digestibility and blood indices of growing turkeys. Nigerian Journal of AnimalProduction, 50(2):1-15. https://doi.org/10.51791/njap. v50i2.3669.

Pourreza, A., Javandel, F., Seidavi, A. and Franco-Robles, E. (2023). The effect of dietary organic acids on performance, carcass characteristics, immunity, blood constitutes and ileal microflora of broiler chickens. Animal Science Papers and Reports, 41(1): 39-48. https://cabidigitallibrary.org/

Reitman, S. and Frankel, S. (1957). A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology, 28(1): 56-63. https://doi.org/10.1093/ajcp/28.1.56

SPSS. (2019). IBM SPSS Statistics for Windows,Version 26.0. Armonk, NY: IBM Corp.

Saad, M.H., Hameed, S.S. and Ali, A.J. (2024). Effect of some essential oil and organic acids on blood picture, lipid profile and intestinal healith of broiler chickens . Iraqi Journal of Agricultural Sciences, 55(6):2006-2011. https://doi.org/10.36103/mtzrsz87

Sabir, P.S., Mirza, R.A., Hamedmin, A.E., and Abdulla, H.S. (2023). Efficacy of peppermint (Mentha pipreitae), basil (Ocimum basilicum) and their combination on growth performance and meat quality of broilers. Diyala Agricultural Sciences Journal, 15(1): 26–33. https://doi.org/10.52951/dasj.23150104

Saleem, K., Saima, Rahman, A., Pasha, T. N., Mahmud, A. and Hayat, Z. (2020). Effects of dietary organic acids on performance, cecal microbiota, and gut morphology in broilers. Tropical Animal Health and Production, 52: 3589-3596. https://doi.org/10.1007/s11250-020-02396-2

Salem, H. and El-Garhy, O.H. (2021). Influence of some organic acids additives on carcass traits, blood biochemistry and economical efficiency in broilers. Annals of Agricultural Science, Moshtohor, 59(5): 61-70. https://doi.org/10.21608/ ASSJM.2021.183648

Saragih, H.T., Susanto, A., Aditya, N.C., Damayanti, S.A.C., Firdaus, A.B.I., Salsabila, N. and Nuriliani, A. (2024). Growth performance of broiler chicken supplemented with water-extracted red dragon fruit [Hylocereus polyrhizus (FAC Weber) Britton Rose]. Jurnal Ilmu Ternak dan Veteriner, 29(4): 208-220. https://doi. org/10.14334/jitv.v29i4.3445

Scicutella, F., Mannelli, F., Daghio, M., Viti, C. and Buccioni, A. (2021). Polyphenols and organic acids as alternatives to antimicrobials in poultry rearing: a review. Antibiotics, 10(8): 1010. https://doi.org/10.3390/antibiotics10081010

Tomar, K., Kumar, R., Yadav, S.P., Sahu, D.S., Roy, D. and Tiwari, S. (2020). Effect of feeding organic acid on blood biochemical parameters in commercial broiler. International Journal of Chemical Studies, 8(6): 260-262. https://doi.org/10.22271/chemi.2020.v8.i6d.10779

Toor, J. and Goel, R. (2022). Poultry farming in India with special reference to Punjab: an overview. Agricultural Science Digest - A Research Journal. https://doi.org/10.18805/ag.D-5540.

Varley, H., Gowenlock, A.H. and Bell, M. (1980). Practical Clinical Biochemistry. William Heinemann Medical Books Ltd., London.

Waghmare, S., Gupta, M., Bahiram, K.B., Korde, J.P., Bhat, R., Datar, Y., Rajora, P., Kodam, M.M., Kaore, M. and Kurkure, N.V. (2025). Effects of organic acid blends on the growth performance, intestinal morphology, microbiota, and serum lipid parameters of broiler chickens. Poultry Science, 104(1): 104546. https://doi. org/10.1016/j.psj.2024.104546

Wotton, I.D.P. (1964). Micro – Analysis in medical Biochemistry. 4th ed. Churchill Livingstone, London, 4:254. https://www.cabidigitallibrary. org/doi/full/10.5555/19651405102