Detection of Lr19/Sr25 in segregating populations of wheat (Triticum aestivum L.) using robust molecular markers
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Authors
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Dharam Pal
IARI-RS, Shimla
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Subhash Chander Bhardwaj
IIWBR-RS, Shimla
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Hanif Khan
IIWBR, Karnal
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Madhu Patial
IARI-RS, Shimla
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Ram Niwas Yadav
IARI-RS, Karnal
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Pratima Sharma
IARI-RS, Shimla
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Shilpi Kumari
IARI-RS, Shimla
Keywords:
Triticum aestivum, rust resistance, Lr19/Sr25, markers, MABAbstract
Marker assisted breeding (MAB) technology has been proved effective
to transfer the genes of interest and also increased the accuracy of
selection in wheat improvement programmes. The Agropyron elongatum
derived 7D.7Ag translocation carrying Lr19/Sr25 is not only effective
against leaf rust pathotypes but also effective against stem rust race
Ug99 and its variants. Therefore, wheat breeding lines carrying Lr19/
Sr25 translocation segment have been developed worldwide. The
present study was aimed for developing wheat genotypes with Lr19/
Sr25 using Xwmc221, PSY1-E1 and Gb DNA markers in segregating
populations of wheat. We could infer that Xwmc221, PSY1-E1 and
Gb are effective molecular markers to tag Lr19/Sr25 in segregating
generations of wheat. The data also revealed consistency between
host pathogen interaction (HPI) test and Xwmc221, PSY1-E1 and Gb
molecular markers for selecting lines with Lr19/Sr25 in segregating
generations. This work not only confirmed the robustness of the
three Lr19/Sr25 markers but also demonstrated the application of
both genotyping and phenotyping in making full-proof selection of
superior progenies with gene of interest in wheat.
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