IN SILICO ANALYSIS PREDICTS AN AMINO ACID PERMEASE FAMILY PROTEIN AS THE PUTATIVE CANDIDATE GENE UNDERLYING A MAJOR QTL FOR TILLER NUMBER IN RICE (Oryza sativa L.)
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Authors
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KEERTHI ISSA, LAKSHMINARAYANA R. VEMIREDDY, ESWARAYYA RAMI REDDY, LAL AHAMED MOHAMMAD AND P. LAVANYA KUMARI
Department of Molecular Biology and Biotechnology, S.V. Agricultural College, Acharya N.G. Ranga Agricultural University (ANGRAU), Tirupati- 517 502
Keywords:
Amino acid permease, Candidate gene Rice, In silico analysis, QTL, Tiller numberAbstract
Rice (Oryza sativa L.) yield and it’s component traits are known to have complex inheritance
and are controlled by several genes with small effects. Although several genomic regions or quantitative
trait loci (QTLs) are identified for a galaxy of traits in rice, only a few candidate genes underlying
these QTLs have been functionally characterized as involvement of the laborious and time-consuming
fine mapping and map-based cloning strategies. However, with the advances in sequencing
technologies, now a wealth of information related to genomics, transcriptomics, proteomics and
metabolomics has been publicly available. Exploiting these databases, in the present investigation,
we predicted amino acid permease (AAP) (LOC_Os01g71720), as a candidate gene underlying a
major effective QTL for tiller number. The candidature of the OsAAP for tiller number QTL has been
endorsed by the literature mining, QTG finder, GO analysis, sequence variation, and gene expression
analysis. Therefore, this OsAAP could be targeted for further functional analysis before being exploited
in future rice breeding.
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