INFLUENCE OF PROCESS PARAMETERS ON CELLULASE PRODUCTION BY Trichoderma viride (MTCC 800) EMPLOYING SOLID STATE FERMENTATION
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Authors
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P. R. KAKDE and S. C. AITHAL *
Department of Microbiology, Dnyanopasak College, Swami Ramanand Teerth Marathwada University (SRTMU), Nanded-431 606
Keywords:
Agricultural biomass, Biofuel, CMCase, FPase, Optimization.Abstract
In this study, the effect of physico-chemical parameters on the production of cellulase
from Trichoderma viride (MTCC 800) were examined using five different agricultural residues
namely banana peel, groundnut shell, corn cob, sugarcane bagasse, and pigeonpea stalk. The
process parameters examined were pretreatment method, supplementation media, initial media
pH, incubation period, temperature, and leaching agent. Among the parameters studied, maximum
cellulase production was obtained on all the substrates treated with 1 N H2SO4 supplemented with
Mandel’s medium of pH 5.5-6 after 6-8 days of incubation at 30-36°C and 0.05 M phosphate
buffer (pH 7.0) as a leaching agent.
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