IDENTIFICATION AND IN SILICO CHARACTERIZATION OF TaGSTU30 AND TaTPS1 GENES IN BREAD WHEAT
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Authors
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AMARDEEP* and T. SINGH
Department of Genetics and Plant Breeding, Kisan (P.G.) College, Chaudhary Charan Singh University, Simbhaoli, Hapur, Uttar Pradesh-245207
Keywords:
Wheat, Drought, GSTU30, TPS1, In silicoAbstract
In case of rice, OsGSTU30 and OsTPS1 genes play a critical role in development, metabolism,
and abiotic stress responses.In the study, it was aimed to identify and characterizeOsGSTU30 and
OsTPS1genes in wheat reference genomic data. Functional domains, physicochemical properties
and subcellular localization of both genes were identified. The homology modeling method was used
to predict the 3D structures and Ramachandran plots were also predicted to evaluate protein structures.
In silico expression profiles of wheat,TaGSTU30 and TPS1 genes at different development tissues
were also identified. Most members of TaGSTU30 and TaTPS1 genes shared similar patterns of
exon/intron structures, including intron phase, intron number, and exon length. Drought responsive
orthologous genes were also identified in recently released reference genomic data of wheat (available
on Ensembl Plants), which were previously identified in rice and Arabidopsis plants. The results of
the study would permit a better understanding of the structure and advancement of the genes and
the encoded proteins and also provide information for the exploitation of variability for the engineering
of these genes.
References
Altschul, S.F., Madden, T.L., Schäffer, A.A.,
Zhang, J., Zhang, Z., Miller, W and Lipman,
D.J. 1997. Gapped BLAST and PSIBLAST:
A new generation of protein
database search programs. Nucleic
Acids Research. 25: 3389-33402.
Arnold, K., Bordoli, L., Kopp, J and Schwede, T.
The SWISS-MODEL workspace: A
web based environment for protein
structure homology modelling.
Bioinformatics. 22:195-201.
Avonce, N., Leyman, B., Mascorro-Gallardo,
J.O., VanDijck, P., Thevelein, J.M and
Iturriaga, G. 2004. The Arabidopsis
trehalose-6-P synthase AtTPS1 gene is a
regulator of glucose, abscisic acid, and
stress signaling. Plant Physiology.136:
–3659.
Biasini, M., Bienert, S., Waterhouse, A., Arnold,
K., Studer, G., Schmidt, T., Kiefer, F.,
Cassarino, T.G., Bertoni, M., Bordoli, L and
Schwede,T.2014.SWISS-MODEL:modelling protein tertiary and quaternary
structure using evolutionary information.
Nucleic Acids Research. 42: W252–W258.
Boyer, J. S. 1982. Plant productivity and
environment.Science. 218: 443–448.
Garg, A.K., Kim, J.K., Owens, T.G., Ranwala, A.P.,
Choi, Y.D., Kochian, L.V and Wu, R.J.
Trehalose accumulation in rice
plants confers high tolerance levels to
different abiotic stresses. Proceedings of
the National Academy of Sciences.99(25):
–15903.
Gasteiger, E., Hoogland, C., Gattiker, A., Duvaud,
S., Wilkins, M.R., Appel, R.D and Bairoch,
A. 2005.Protein identification and analysis
tools on the ExPASy server. In: The
Proteomics Protocols Handbook, J.M.
Walker (Editor), Humana Press, USA. pp.
–607.
George, S., Venkataraman, G and Parida, A.
A chloroplast-localized and auxininduced
glutathione S-transferase from
phreatophyteProsopis juliflora confer
drought tolerance on tobacco.
Journal of Plant Physiology. 167(4): 311–
Higo, K., Ugawa, Y., Iwamoto, M and Korenaga,
T. 1999. Plant cis-acting regulatory DNA
elements (PLACE) database:1999. Nucleic
Acids Research. 27: 297–300.
Ingram, J and Bartels, D. 1996. The molecular
basis of dehydration tolerance in plants.
Annual Review of Plant Physiology and
Plant Molecular Biology. 47(1): 377–403.
Jang, I.C., Oh, S.J., Seo, J.S., Choi, W.B., Song,
S.I., Kim, C.H., Kim, Y.S., Seo, H.S., Choi,
Y.D., Nahm, B.H and Kim, J.K. 2003.
Expression of a bifunctional fusion of the
Escherichia coli genes for trehalose-6-
phosphate synthase and trehalose-6-
phosphate phosphatase in transgenic rice
plants increases trehalose accumulation
and abiotic stress tolerance without
stunting growth. Plant Physiology. 131:
–524.
Jatoi, W.A., Baloch, M.J., Kumbhar, M.B., Khan,
N.U and Kerio, M.I. 2011. Effect of water
stress on physiological and yield
parameters at anthesis stages in elite
spring wheat cultivars.Sarhad Journal of
Agriculture. 27(1): 59–65.
Ji, W., Zhu, Y., Li, Y., Yang, L., Zhao, X., Cai, H
and Bai, X. 2010. Over-expression of a
glutathione S-transferase gene, GsGST,
from wild soybean (Glycine soja)
enhances drought and salt tolerance in
transgenic tobacco. Biotechnology Letters.
(8):1173–1179.
Kumar, A., Kumar, S., Kumar, A., Sharma, N.,
Sharma, M., Singh, K.P., Rathore, M and
Gajula, M.N.V.P. 2018-I. Homology
modeling, molecular docking and
molecular dynamics based functional
insights into rice urease bound to urea.
Proceedings of the National Academy of
Sciences. 88: 1539–1548.
Kumar, A., Kumar, S., Kumar, U., Suravajhala, P
and Gajula, M.N.V.P. 2016. Functional and
structural insights into novel DREB1A
transcription factors in common wheat
(Triticum aestivum L.): A molecular
modeling approach. Computational
Biology and Chemistry. 64: 217–216.
Kumar, A., Sharma, M., Gahlaut, V., Nagaraju,
M., Chaudhary, S., Kumar, A., Tyagi, P.,
Gajula, M.N.V.P and Singh, K.P. 2019.
G e n o m e - w i d e i d e n t i f i c a t i o n ,
characterization, and expression profiling
of SPX gene family in wheat. International
Journal of Biological Macromolecules.
: 17–32.
Kumar, A., Sharma, M., Kumar, S., Tyagi, P., Wani,
S.H., Gajula, M.N.V.P and Singh, K.P.
-II. Functional and structural insights
into candidate genes associated with
nitrogen and phosphorus nutrition in wheat
(Triticum aestivum L.). International
Journal of Biological Macromolecules. 118:
–91.
Kumar, S., Asif, M.H., Chakrabarty, D., Tripathi,
R.D., Dubey, R.S and Trivedi, P.K. 2013.
Expression of a rice Lambda class of
glutathione S-transferase, OsGSTL2, in
Arabidopsis provides tolerance to heavy
metal and other abiotic stresses. Journal
of Hazardous Materials. 15: 248-249.
Lawlor, D.W and Cornic, G. 2002.Photosynthetic
carbon assimilation and associated
metabolism in relation to water deficits in
higher plants. Plant, Cell and Environment.
(2): 275-294.
Li, H.W., Zang, B.S., Deng, X.W and Wang, X.P.
Overexpression of the trehalose-6-
phosphate synthase gene OsTPS1
enhances abiotic stress tolerance in
rice. Planta.234(5):1007-1018.
Mitchell,J.H.,Siamhan, D., Wamala, M.H.,
Risimeri, J.B., Chinyamakobvu, E.,
Henderson, S.A and Fukai, S.1998. The
use of seedling leaf death score for
valuation of drought resistance of rice.
Field Crops Research. 55: 129–139.
Pettersen, E.F., Goddard, T.D., Huang, C.C.,
Couch, G.S., Greenblatt, D.M., Meng, E.C
and Ferrin, T.E. 2004.UCSF chimera
visualization system for exploratory
research and
analysis.Journal of Computational
Chemistry. 25: 1605–1612.
Seppa¨nen, M.M., Cardi, T., Hyo¨kki, M.B and
Pehu, E. 2000. Characterization and
expression of cold-induced glutathione Stransferase
in freezing tolerant Solanum
commersonii, sensitive S. tuberosum and
their interspecific somatic hybrids. Plant
Science.153(2): 125-133.
Singh, D and Laxmi, A. 2015. Transcriptional
regulation of drought response: A tortuous
network of transcriptional factors.Frontiers
of Plant Science. 6: 895. https://doi.org/
3389/fpls.2015.00895.
Srivastava, D., Verma, G., Chauhan, A.S., Pande,
V and Chakrabarty, D. 2019. Rice (Oryza
sativa L.) tau class glutathione Stransferase
(OsGSTU30) overexpression
in Arabidopsis thaliana modulates a
regulatory network leading to heavy metal
and drought stress tolerance. Metallomics.
(2): 375–389.
Tiwari, M., Srivastava, S., Singh, P.C., Mishra,
A.K and Chakrabarty, D. 2020.Functional
characterization of tau class glutathione-
S-transferase in rice to provide tolerance
against sheath blight disease.3Biotech.
(3):84.
Tripathi, A., Indoliya, Y., Tiwari, M., Tiwari, P.,
Srivastava, D., Kumarverma, P., Verma, S.,Gautam, N and Chakrabarty, D. 2014.
Transformed yeast(Schizosaccharomyces
pombe) over expressing rice Tau class
glutathione S-transferase (OsGSTU30 and
OsGSTU41) shows enhanced resistance
to hexavalent chromium. Metallomics. 6(8):
–1557.
Warde-Farley, D., Donaldson, S.L., Comes, O.,
Zuberi, K., Badrawi, R., Chao, P., Franz,
M., Grouios, C., Kazi, F., Lopes, C.T.,
Maitland, A., Mostafavi, S., Montojo, J.,
Shao, Q., Wright, G., Bader, G.D and
Morris, Q. 2010. The Gene MANIA
prediction server: Biological network
integration for gene prioritization and
predicting gene function, Nucleic Acids
Research. 38: W214-W220.
Wilce, M.C.J and Parker M.W. 1994. Structure
and function of glutathione Stransferases.
Biochimicaet Biophysica
Acta. 1205: 1–18.
Xu, J., Tian, Y.S., Xing, X. J., Peng, R.H., Zhu, B.,
Gao, J.J and Yao, Q.H. 2016. Overexpression
of AtGSTU19 provides
tolerance to salt, drought and methyl
viologen stresses in Arabidopsis.
Physiologia Plantarum. 156(2): 164–175.
Yang, G., Wang, Y., Xia, D., Gao, C., Wang, C
and Yang, C. 2014. Overexpression of a
GST gene (ThGSTZ1) from
Tamarixhispida improves drought and
salinity tolerance by enhancing the ability
to scavenge reactive oxygen species.Plant
Cell, Tissue and Organ Culture.117(1): 99-
Yordanov, I., Velikova, V and Tsonev, T. 2000.
Plant responses to drought, acclimation,
and stress tolerance.Photosynthetica.
(2): 171-186.
Zhu, J.K. 2002. Salt and drought stress signal
transduction in plants. Annual
Review of Plant Biology. 53: 247-273.
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