CULTURAL AND MORPHOLOGICAL VARIABILITY OF CORYNESPORA CASSIICOLA (BERK. AND CURT.) WEI. CAUSING TARGET SPOT DISEASE IN COTTON
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Abstract
Cultural and morphological variability of Corynespora cassiicola (Berk. and Curt.) Wei. causing
target spot of cotton, was studied on different growth media. Ten different media viz., Potato Dextrose
Agar (PDA), Potato Sucrose Agar (PSA), Carrot Dextrose Agar (CDA), Carrot Sucrose Agar (CSA),
Oat Meal Agar (OMA), Lactose Casein Agar (LCA), Lactose Peptone Agar (LPA) and different leaf
extract media viz., Cotton Leaf Extract Agar (CLEA), Blackgram Leaf Extract Agar (BgLEA), Greengram
Leaf Extract Agar (GgLEA) were used to find out the media which enables pathogen to grow fast and
to identify the best media for good sporulation. Among the different media tested,CLEA media
promotedthe highest radial growth (7.83cm) having more growth rate (0.54 mm per hr) followed by
BgLEA with radial growth of 6.80cm and growth rate of 0.47mm per hr; lowest radial growth observed
in CAS (4.20 cm) with growth rate of 0.29 mm per hr. Highest sporulation observed in CLEA (394
x104±10.8) followed by LPA (132 x104±22.1) and lowest was observed in GgLEA (11.2 x104±2.8).
Conidial morphology varied in the different media tested, lowest conidial length was observed in
CLEAmedium(32.83 ±3.33μm) whereas highest in LPA medium(91.58±3.50μm);narrowest conidial
width in CLEA medium (7.36±1.79μm) and broadest conidial length in BgLEA medium (12.42±2.02μm).
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