MARKER ASSISTED BREEDING WITHOUT BACKGROUND SELECTION FOR IMPROVEMENT OF DROUGHT TOLERANCE IN A BREAD WHEAT CULTIVAR
133 / 75
Authors
-
AMARDEEP* and T. SINGH
Department of Genetics and Plant Breeding, Kisan (P.G.) College, Chaudhary Charan Singh University, Simbhaoli, Hapur- 245 207
Keywords:
Wheat, HD2967, MAS, QTL, foreground selection, phenotypic selectionAbstract
Drought stress is a major limiting factor in wheat production. The study was conducted during
rabi crop seasons from 2010-11 to 2019-20 by utilizing a marker-assisted selection scheme to
introgress a major drought insensitive yield QTL (Qyld.csdh.7AL) from SQ1 into Indian wheat cultivar
(HD2967) using SSR marker Xwmc273.3, leading to the development of 3 improved wheat lines
having relatively higher grain yield under water stress condition. HD2967, a high yielding wheat cultivar
which has been proved as a successful variety for northwestern plain zone (NWPZ) of India but
sensitive to drought stress, was used as the recurrent parent. For introgression of this major yield
QTL, a cross between HD2967 and SQ1 was made and subsequently two back crosses with recurrent
parent (HD2967) were attempted during the off/crop seasons. QTL was validated in the BC1F2
population using foreground selection followed by the collection of phenotypic data (grain weight/ear,
grain weight/plant, plant biomass, flag leaf chlorophyll content, and harvest index) of two groups of
plants having SQ1 and HD2967 alleles for the QTL. After foreground and phenotypic selection,
homozygous superior lines containing the SQ1 allele for the QTL Qyld.csdh.7AL were selected in
BC2F4 population. The high yield in the selected plants was attributed to grain weight/plant, biomass/
plant, and flag leaf chlorophyll content.
References
garwal, P.K., Agarwal, P., Reddy, M.K and
Sopory, S.K. 2006. Role of DREB
transcription factors in abiotic and biotic
stress tolerance in plants. Plant Cell
Reports.25: 1263-1274.
Agbicodo, E.M., Fatokun, E.C.A., Muranaka,
E.S., Visser, R.G.F and Lindenvander, C.G.
Breeding drought tolerant cowpea:
constraints, accomplishments, and future
prospects. Euphytica. 167: 353-370.
Ashley, J.1993.Drought and crop adaptation. In:
Dryland farming in Africa (Rowland, J.R.J.
Editor). UK, Macmillan Press Ltd. pp. 46-
Bansal, R., Pradheep, K., Kumari, J., Kumar, S.,
Yadav, M.C., Gurung, B., Kumari, N.K.P and
Rana, J.C. 2016. Physiological and
biochemical evaluation for drought
tolerance in wheat germplasm collected from
arid western plains of India. Indian Journal
of Biochemistry and Biophysics. 53: 212-
Blum, A.1988.Plant breeding for stress
environments. Boca Raton, Florida, USA.
CRC Press Inc. pp.62-70.
Blum, A.2005. Drought resistance, water use
efficiency and yield potential- are they
compatible, dissonant, or mutually
exclusive? Australian Journal of Agricultural
Research. 56: 1159-1168.
MARKER ASSISTED BREEDING WITHOUT BACKGROUND SELECTION IN A BREAD WHEAT CULTIVAR
Bohnert, H.J., Nelson, D.E and Jensen,
R.G.1995.Adaptations to environmental
stresses. The Plant Cell. 7: 1099-1111.
Boussakouran, A., Sakar, E.H., Yamani, M.E and
Rharrabti, Y.2019. Morphological traits
associated with drought stress tolerance in
six Moroccan Durum Wheat varieties
released between 1984 and 2007. Journal
of Crop Science and Biotechnology. 22:
-353.
Chen, S., Lin, X.H., Xu, C.G and Zhang,
Q.2000.Improvement of bacterial blight
resistance of ‘Minghui 63’, an elite restorer
line of hybrid rice, by molecular markerassisted
selection.Crop Science. 40: 239–
Gautam, T., Amardeep, Saripalli, G., Rakhi,
Kumar, A., Gahlaut, V., Gadekar, D.A., Oak,
M., Sharma, P.K., Balyan, H.S and Gupta,
P.K.2020. Introgression of a drought
insensitive grain yield QTL for improvement
of four Indian bread wheat cultivars using
marker assisted breeding without
background selection. Journal of Plant
Biochemistry and Biotechnology. Doi: https:/
/doi.org/10.1007/s13562-020-00553-0.
Gupta, H.S., Agrawal, P.K., Mahajan, V., Bisht,
G.S., Kumar, A., Verma, P., Srivastava, A.,
Saha, S., Babu, R., Pant, M.C and Mani,
V.P.2009. Quality protein maize for
nutritional security: rapid development of
short duration hybrids through molecular
marker assisted breeding. Current Science.
:230-237.
Gupta, P.K., Langridge, P and Mir, R.R. 2010.
Marker-assisted wheat breeding: Present
status and future possibilities. Molecular
Breeding. 26: 145-161.
Izanloo, A., Condon, A.G., Langridge, P., Tester,
M and Schnurbusch, T.2008. Different
mechanisms of adaptation to cyclic water
stress in two South Australian bread wheat
cultivars. Journal of Experimental Botany.
(12): 3327-3346.
Mitra, J. 2001.Genetics and genetic improvement
of drought resistance of crop plants.
Current Science. 80: 758–763.
Mollasadeghi, V., Imani, A.A., Shahryari, R and
Khayatne Zhad, M. 2011.Correlation and
path analysis of morphological traits in
different wheat genotypes under end
drought stress condition. Middle-East
Journal of Scientific Research. 7(2): 221-
Mwadzingeni, L., Shimelis, H., Dube, E., Laing,
M.D and Tsilo, T.J.2016. Breeding wheat for
drought tolerance: Progress and
technologies. Journal of Integrative
Agriculture. 15(5): 935-943.
Quarrie, S.A., Quarrie, S.P., Radosevic, R.,
Rancic, D., Kaminska, A.S., Barnes, J.D.,
Leverington, M., Ceolini, C and Dodig,
D.2006. Dissecting a wheat QTL for yield
present in a range of environments: from
the QTL to candidate genes. Journal of
Experimental Botany. 57(11): 2627–2637.
Quarrie, S.A., Steed, A., Calestani, C.,
Semikhodskii, A., Lebreton, C., Chinoy, C.,
Steele, N., Pljevijakusic, D., Waterman, E.,
Weyen, J., Schondelmaier, J., Habash, D.Z.,
Farmer, P., Saker, L., Clarkson, D.T.,
Abugalieva, A., Yessimbekova, M.,
AMARDEEP and SINGH
Turuspekov, Y., Abugalieva, S., Tuberosa,
R., Sanguineti, M.C., Hollington, P.A.,
Aragues, R., Royo, A and Dodig, D. 2005. A
high-density genetic map of hexaploid
wheat (Triticum aestivum L.) from the cross
Chinese Spring x SQ1 and its use to
compare QTLs for grain yield across a
range of environments. Theoretical and
Applied Genetics. 110(5): 865-880.
Rai, N., Bellundagi, A., Kumar, P.K., Kalasapura
Thimmappa, R., Rani, S., Sinha, N., Krishna,
H., Jain, N., Singh, G.P., Singh, P.K and
Chand, S. 2018. Marker assisted backcross
breeding for improvement of drought
tolerance in bread wheat (Triticum aestivum
L. emThell). Plant Breeding. 137(4): 514-
Richards, R.A., Rawson, H.M and Johnson,
D.A.1986.Glaucousness in wheat: Its
development and effect on water-use
efficiency, gas exchange and photosynthetic
tissue temperatures. Australian Journal of
Plant Physiology. 13(4): 465-473.
Saghai-Maroof, M.A., Soliman, K.M., Jorgensen,
R.A and Allard, R.W.1984. Ribosomal DNA
spacer-length polymorphisms in barley:
Mendelian inheritance, chromosomal
location and population dynamics.
Proceedings of the National Academy of
Sciences, USA. 81:8014–8018.
Sallam, A., Alqudah, A.M., Dawood, M.F.A.,
Baenziger, P.S and Börner, A.2019. Drought
Stress Tolerance in Wheat and Barley:
Advances in Physiology, Breeding and
Genetics Research. International Journal of
Molecular Sciences. 20(13):3137-3138.
doi:10.3390/ijms20133137.
Smith, M.E., Coffman, W.R and Baker, T.C.1990.
Environmental effects on selection under
high and low-input conditions. In: Genotypeby-
Environment Interaction and Plant
Breeding. Kang, M.S.(Editor). Baton Rouge:
Louisiana State University. 261–272.
Srivastava, A., Srivastava, P.,& Sharma, A and
Sarlach, R.S. 2017.Canopy temperature an
effective measure of drought stress
tolerance in RIL population of wheat.
Vegetos- An International Journal of Plant
Research.30:59.doi:10.5958/2229-
2017.00011.8.
Turner, N.C., Wright, G.C and Siddique,
K.H.M.2001. Adaptation of grain legumes
(pulses) to water limited environments.
Advances in Agronomy. 71: 193-231.
Umezawa, T., Fujita, M., Fujita, Y., Yamguchi-
Shinozaki, K and Shinozaki, K.2006.
Engineering drought tolerance in plants:
discovering and tailoring genes to unlock
the future.Current Opinion in Biotechnology.
(2): 113-122.
Downloads
Submitted
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The author owns the article's copyright until the article is accepted for publication. After acceptance, the author(s) assigns the article's copyright jointly to both the authors and the Publishers of the Journal of Research ANGRAU (ANGRAU) and licensed under a Creative Commons Attribution-Non Commercial-Share Alike 4.0 International License.
