MARKER ASSISTED BREEDING WITHOUT BACKGROUND SELECTION FOR IMPROVEMENT OF DROUGHT TOLERANCE IN A BREAD WHEAT CULTIVAR
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Authors
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AMARDEEP* and T. SINGH
Department of Genetics and Plant Breeding, Kisan (P.G.) College, Chaudhary Charan Singh University, Simbhaoli, Hapur- 245 207
Keywords:
Wheat, HD2967, MAS, QTL, foreground selection, phenotypic selectionAbstract
Drought stress is a major limiting factor in wheat production. The study was conducted during
rabi crop seasons from 2010-11 to 2019-20 by utilizing a marker-assisted selection scheme to
introgress a major drought insensitive yield QTL (Qyld.csdh.7AL) from SQ1 into Indian wheat cultivar
(HD2967) using SSR marker Xwmc273.3, leading to the development of 3 improved wheat lines
having relatively higher grain yield under water stress condition. HD2967, a high yielding wheat cultivar
which has been proved as a successful variety for northwestern plain zone (NWPZ) of India but
sensitive to drought stress, was used as the recurrent parent. For introgression of this major yield
QTL, a cross between HD2967 and SQ1 was made and subsequently two back crosses with recurrent
parent (HD2967) were attempted during the off/crop seasons. QTL was validated in the BC1F2
population using foreground selection followed by the collection of phenotypic data (grain weight/ear,
grain weight/plant, plant biomass, flag leaf chlorophyll content, and harvest index) of two groups of
plants having SQ1 and HD2967 alleles for the QTL. After foreground and phenotypic selection,
homozygous superior lines containing the SQ1 allele for the QTL Qyld.csdh.7AL were selected in
BC2F4 population. The high yield in the selected plants was attributed to grain weight/plant, biomass/
plant, and flag leaf chlorophyll content.
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