NUTRITIONAL AND ANTIOXIDANT POTENTIAL OF LYOPHILIZED WHEAT GRASS JUICE AND SHOOT POWDERS


572 / 246

Authors

  • N.M. JABEEN*, POOJA YADAV, MAHADEVA NAIKA, MALLESHA and N. DEVANNA Department of Food Technology, JNTUA-Oil Technological and Pharmaceutical Research Institute, Anantapuramu – 515 005

Keywords:

Phytochemicals, HPLC, Polyphenols, DPPH activity

Abstract

Young wheatgrass is rich in vitamins, minerals, chlorophyll, bioactive compounds, and fiber. In this
study, phytochemicals, proximate analysis, amino acids, individual polyphenols, minerals, and antioxidants
of lyophilized wheatgrass juice powder and shoot powder were estimated. Both the samples (juice and shoot
powders) showed the presence of phytochemicals such as alkaloids, tannins, cardio-glycosides, coumarins,
flavonoids, carbohydrates, etc. Further more, HPLC analysis of individual polyphenols showed that the juice
powder had significantly higher (P <0.05%) quercitin, sinnapic acid, chlorophyll, and essential amino acids
like valine, threonine. Wheat grass juice powder have shown significantly higher (p<0.05%) Fe and Zn than
shoot powder. Juice powder had significantly more (P<0.05) total polyphenols (15.20±1.0 mg/g), total flavonoids
(6.27±0.68 mg/g) than shoot powder, and DPPH activity with IC50 1.48±0.15 mg/g. This study highlights the
nutritional benefits of wheatgrass juice powder as lyophilization could reduce losses of nutrients and antioxidants.
These findings confirm that lyophilized wheatgrass juice powder is medicinally important with bioactive
compounds, antioxidant capacity, and have significant potential to incorporate in functional food products
those can be used to enhance the health benefits of consumer.

References

1. Alitheen, N.B, Oon C.L, Keong, Y.S, Chuan, T.K,

2. H.K and Yong, H.W. 2011. Cytotoxic

3. effects of commercial wheatgrass and

4. fibre towards human acute promyelocytic

5. leukaemia cells (HL60). Pakistan Journal

6. of Pharmaceutical Sciences. 24(3): 243-

7. Anand Rojoria 2015. Phytochemical analysis

8. and estimation of major bioactive

9. compounds from Triticum aestivum

10. L.grass with antimicrobial potential.

11. Pakistan Journal of Pharmacy. 28

12. (6):2221-2225.

13. Association of Official Agricultural Chemists.

14. (AOAC). 2005. Official Methods of

15. Analysis, 18th Edition. Washington, DC.

16. pp. 256.

17. Ayoola, G.A, Coker, H.A.B., Adesegun, S.A,

18. Adepoju-Bello, A.A, Obaweya, K, Ezennia

19. E.C and Atangbayila. 2008. Phytochemical

20. screening and antioxidant

21. activities of some selected medicinal

22. plants used for malaria therapy in South

23. Western Nigeria. Tropical Journal of

24. Pharmaceutical Research. (7):1019-

25. Bar-sela, G, Tsalic, M, Fried, G and Goldberg,

26. H. 2007. Wheat grass juice may improve

27. hematological toxicity related to

28. chemotherapy in breast cancer patients.

29. A pilot study. Journal of Nutrition and

30. Cancer. 58: 43-48.

31. JABEEN et al.

32. Calzuola, I., Marsili,V and Gianfranceschi,

33. G.L.2004.Synthesis of antioxidants in

34. wheat sprouts. Journal of Agriculture and

35. Food Chemistry. 52: 5201-5206.

36. Chauhan, M. 2014. A pilot study on wheat grass

37. juice for its phytochemical, nutritional and

38. therapeutic potential on chronic diseases.

39. International Journal of Chemistry. 2(4):

40. -34.

41. Dey, S., Sarkar, R. Ghosh, P, Khatun, R., Ghorai

42. K, Choudhari, R, Ahmad, R, Gupta, P and

43. Mukopadhya, A. 2006. Effect of wheat

44. grass juice in supportive care of terminally

45. ill cancer patients-A tertiary cancer centre

46. experience from India. Journal of Clinical

47. Oncology ASCO meeting proceedings

48. Part I. 18(1):8634.

49. Dhawliwal, H. S., Sharma, N, Bano, A, Kumar,

50. S and Sharma V. 2015.Non–Enzymatic

51. assay based in vitro Antioxidant Activity

52. and Phytochemical screening of Freezedried

53. wheat seedlings juice powder.

54. Nature´s Finest Medicine-Part-II. International

55. Journal of Chemistry. 6(9):

56. -46.

57. Endo, Y, Usuki, R and Kaneda, T. 1985. antioxidant

58. effects of chlorophyll and

59. pheophytin on the auto-oxidation of oil in

60. the dark. The mechanism of oxidative

61. chlorophyll. Journal of the American Oil

62. Chemist’s Society. 62:1387-1390.

63. Falconi.G., Calzuola L.M, Marsilli. V and

64. Gianfranceschi, G.2002. Antioxidant

65. activity of wheat sprout extraction vitro:

66. inhibition of DNA oxidative damage.

67. Journal of Food Science. 67: 2918-2922.

68. Garima Shakya, Sankar Pajaaniradje,

69. Muddasarul Hoda, Varalakshmi Durairaj,

70. Rukkumani Rajagopalan. 2014. GC-MS

71. analysis, in vitro antioxidant and cytotoxic

72. studies of wheat grass extract. American

73. Journal of Phytomedicine and Clinical

74. Therapeutics. (7):877-893.

75. Ghumman A., Narpinder Singh and Amritpal

76. Kaur. 2017. Chemical, nutritional and

77. phenolic composition of wheat grass and

78. pulse shoots. International Journal of Food

79. Science and Technology. pp.1-10.

80. Ghumman, A., Kaur, A and Singh, N .2016.

81. Impact of germination on flour, protein and

82. starch characteristics of lentillns culinaris

83. and hoarse gram (Macrotylo mauniflorum

84. L.) lines. International Journal of Food

85. Science and Technology.65:137-144.

86. Hartl, A.W and Stenzel, R. 2007. Development

87. of a method for the determination of

88. citrinin in barley, rye and wheat by solid

89. phase extraction on amino propyl

90. columns and HPLC-FLD. Mycotoxin

91. Research journal. 23:127-131.

92. Hiscox, J.D and Israelstam, G.F.1979.A method

93. for the extraction of chlorophyll from leaf

94. tissue without maceration. Canadian

95. Journal of Botany. 57.1332-1334.

96. Kamat, J.P. Boloor, K, K and Devasagayam, T,

97. P. 2000. Chlorophyllin as an effective

98. antioxidant against membrane damage in

99. vitro and ex vivo. Biochimicia et Bio-

100. Physica Acta Journal. 1487. 113-127.

101. NUTRITIONAL AND ANTIOXIDANT POTENTIAL OF LYOPHILIZED WHEAT GRASS JUICE AND SHOOT POWDERS

102. Kokate, C. K, Purohit, A.P and Gokulae,

103. S.B.2001. Text book of Pharmacognosy.

104. Nirali Prakashan Publications, New Delhi,

105. India. pp.21-28.

106. Kulkarni S. D, Acharya, R., Nair, A.G.C, Rajurkur

107. N.S and Reddy A.V.R. 2006. Determination

108. of elemental concentration profiles in

109. tender wheat grass using instrumentation

110. neuron activation analysis. Food Chemistry.

111. :699-707.

112. Lanfar-Marquez, U., Barros, R and Sinnecker,

113. P. 2005. Antioxidant activity of chlorophylls

114. and their derivatives. Food Research

115. International. 38:885-891.

116. Makkar H.P.S. 2003.Effect of fate of tannins in

117. ruminant animals. Adaptation of tannins

118. and strategies to overcome detrimental

119. effects of feeding tanning rich feeds.

120. Small Ruminant Research Journal.

121. (3):241-256.

122. Marwaha, R.K., Bansal, D., Kaur, S and Trehan,

123. A. 2004. Wheat grass juice reduces the

124. transfusion requirement in patients with

125. Thalamessia. A major pilot study. Indian

126. Paediatrics.41:716-720.

127. Matthew, S., Wheal, Teresa, O., Fowles and

128. Lyndon T. Palmer. 2011. A cost-effective

129. acid digestion method using closed

130. polypropylene tubes for inductively

131. coupled plasma optical emission

132. spectrometry (ICP-OES) analysis of

133. plant essential elements. Analytical

134. Methods. 3: 2854–2863.

135. Nazmul Hasan, Md., Akhtaruzaman, M and Md.

136. Zakir Sultan. 2013. Estimation of Vitamins

137. B-Complex (B2, B3, B5 and B6) of some

138. leafy vegetables indigenous to

139. Bangladesh by HPLC Method. Journal of

140. Analytical Sciences, Methods and

141. Instrumentation. (3): 24-29.

142. Rajesh, M and Ramesh, B. 2011. A study on

143. wheat grass and its nutritional value.

144. Journal of Food Science and Quality

145. Management.2:1-8.

146. Rattanamanee Chomchan, Sunisasiripongvutikorn,

147. Panupongputtarak and Rungtip

148. Rattanapon. 2016. Investigation of

149. phytochemical constituents, phenolics

150. profiles and antioxidant activities of rice

151. grass compared to wheat grass juice.

152. Journal of Functional Foods and Health

153. Diseases. 6 (12): 822-835.

154. Ross, K.A., Beta, T Arntfild and S.D .2009. A

155. comparative study on the phenolic acids

156. identified and quantified in dry beans using

157. HPLC as affected by different extraction

158. and hydrolysis methods. Journal of Food

159. Chemistry.113: 336-344.

160. Schwarz, K. Bertelsen, G. Nissen, L.R, Gardner,

161. P.T, Heinonen, M, I, Hopia, A, Huynh-Ba

162. T, Lambelet P, Mc Phail, D, Skibsted, L.H

163. and Tijburg, L. 2001.Investigation of plant

164. extracts for the protection of processed

165. foods against lipid oxidation. Comparision

166. of antioxidant assays based on radical

167. scavenging. Lipid oxidation and analysis

168. of the principal antioxidant compounds.

169. JABEEN et al.

170. Europian Food Research Technology.

171. : 319-328.

172. Singh, N., Verma, P and Pandey, B. R.2012.

173. Therapeutic potential of organic Triticum

174. aestivum Linn (wheat grass) in prevention

175. and treatment of chronic diseases- An

176. overview. International Journal of

177. Pharmaceutics Sciences and Drug

178. Research. 4:10-14.

179. Smith, B.H. 2000. Generalization of spatially

180. variant apodization to non-integral Nyquist

181. sampling rates. IEEE Trans image

182. process. Journal of Pain. 9:1088-1093.

183. Suriyavathana, M., Roopavathi, I. and Vinuvijayan.

184. Phytochemical characterization

185. of triticum aestivun Journal of

186. Pharmacognosy and Phytochemistry.

187. (1):283-286.

188. Suriyavathana, M. and Roopavathi, I. 2016.

189. Evaluation of antioxidants level in Triticum

190. aestivum. Journal of Medical Plants

191. Studies. 4(5): 18-23.

192. Swathi, P., Sushma, D., Indira, R and Alka Gand

193. Mamta, D.2010. Multitude potential of

194. wheat grass juice (green blood): An

195. Overview Chronicle of Young Scientists.

196. (2):23-28.

197. Thompson, L.U.1994. Antioxidants and

198. hormone-mediated health benefits of

199. whole grains. Critical Reviews in Food

200. Science Nutrition. 34: 473-97.

201. Varalakshmi Durairaj, Muddasarul Hoda,

202. Garima Shakya, Sankar Pajaniradje

203. Preedia Babu, Rukkumani Rajagopalan.

204. Phytochemical screening and

205. analysis of antioxidant properties of

206. aqueous extract of wheat grass. Asian

207. Pacific Journal of Tpropical Medicine.

208. (1):S398-S404.

209. Walters, R. 1992. The alternative cancer therapy

210. book. New York: Avery Publishing Group.

211. pp. 299-308.

212. Yang F, Basu T; K, and Ooraikul B.2001.Studies

213. on germination conditions and antioxidant

214. content of wheat grass. International

215. Journal of Food Science Nutrition. 52:319-

216. Zago M. P., Verstraetan, S. V and Oteiza, P.I.

217. zinc in the prevention of Fe 2+

218. initiated lipid and protein oxidation.

219. Biological Research journal. 33.143-150.

220. Zang, Z-Q, Xiang, J-J and Zhou, L-M; 2015.

221. Antioxidant activity of three components

222. of wheat grass leaves, ferulic acid,

223. flavonoids and ascorbic acid. Journal of

224. Food Science and Technology. 52(11):

225. -7304.

226. Zhishen, J., Mengcheng, T and Jianming.1999.

227. The determination of flavonoid contents

228. in mulberry and their scavenging effects

229. on superoxide radicals. Journal of Food

230. Chemistry. 64: 555-559.

Downloads

Published

30-06-2020

How to Cite

N.M. JABEEN*, POOJA YADAV, MAHADEVA NAIKA, MALLESHA and N. DEVANNA. (2020). NUTRITIONAL AND ANTIOXIDANT POTENTIAL OF LYOPHILIZED WHEAT GRASS JUICE AND SHOOT POWDERS. The Journal of Research ANGRAU, 48(2), 07-22. https://epubs.icar.org.in/index.php/TJRA/article/view/133667