BIOCHEMICAL ANALYSIS OF RESISTANT AND SUSCEPTIBLE COTTON GENOTYPES AGAINST ALTERNARIA AND CORYNESPORA


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Authors

  • D. MAHESH Department of Plant Pathology, Agricultural College, Acharya N.G. Ranga Agricultural University, Bapatla - 522 101
  • S. L. BHATTIPROLU Department of Plant Pathology, Agricultural College, Acharya N.G. Ranga Agricultural University, Bapatla - 522 101
  • V. PRASANNA KUMARI Department of Plant Pathology, Agricultural College, Acharya N.G. Ranga Agricultural University, Bapatla - 522 101
  • CH. CHIRANJEEVI Department of Plant Pathology, Agricultural College, Acharya N.G. Ranga Agricultural University, Bapatla - 522 101

https://doi.org/10.58537/jorangrau.2024.52.1.05

Keywords:

Alternaria, Corynespora, Cotton, Peroxidase, Phenols

Abstract

Cotton leaf samples of 10 genotypes LAHB-1, L799, L 389, L770 and LPS 141 (resistant); L 1060, L 2551, L 2503, L 397 and L 2559 (susceptible) along with one control (Jaadoo BG II hybrid) against Alternaria and Corynespora were analyzed at flowering and boll development stages for the estimation of total phenols, total proteins, total sugars, reducing sugars, nonreducing sugars, chlorophyll and peroxidises. Results showed that all the biochemical parameters,except peroxidase enzyme, showed decreasing trend from flowering to boll development stage. There was significant reduction in phenols at flowering stage (16.23% to 53.30%) and at boll development stage (22.71% to 58.37%), total proteins at flowering stage
(16.91% to 58.19%) and at boll development stage (9.51% to 51.43%), total sugars at flowering stage (15.71% to 51.32%) and at boll development stage (28.10% to 65.05%), reducing sugars at flowering stage (9.59% to 53.00%) and at boll development stage (35.56% to 69.93%), nonreducing sugars at flowering stage (18.38% to 49.53%) and at boll development stage (10.53% to 92.84%) and total chlorophyll at flowering stage (24.80% to 48.46%) and at boll development stage (9.19% to 32.86%). However, there was increase in peroxidase content at flowering stage (59.94% to 225.35%) and at boll development stage (38.79% to 138.79%) as the disease severity increased.

References

1. Agarwal, M.L., Satish, K., Goel, A.K and Tayal,

2. M.S. 1982. Biochemical analysis in leaf

3. spot disease of turmeric. Some

4. hydrolyzing enzymes and related

5. chemical metabolites. Indian

6. Phytopathology. 35: 438-441.

7. Arnon, D.I. 1949. Copper enzymes in isolated

8. chloroplasts.Polyphenoloxidase in Beta

9. vulgaris. Plant Physiology. 24: 1-15.

10. Bhattiprolu, S.L and Monga, D. 2018.Effect of

11. weather parameters on the development

12. of Alternaria leaf spot and grey mildew

13. in cotton.Journal of Agrometeorology. 20

14. (4): 315-318.

15. Gerhardt, P., Murray, R.G.E., Wood, W.A and

16. Krieg, N.R. 1994. Methods for General

17. and Molecular Bacteriology. ASM,

18. Washington DC.

19. Hagan, A.K and Sikora, E.J. 2012. Leaf spot

20. management in Alabama cotton,control

21. of potash- incited leaf spot diseases and

22. Corynespora leaf spot.Plant Pathology

23. Series.Extension Plant Pathology. pp.

24. -962.

25. Hammerschimidt, R., Nuckless, E.M and Kuc,

26. J. 1982. Association of enhanced

27. peroxidase activity with induced systemic

28. resistance of cucumber to Colletotrichum

29. lagenarium. Physiological Plant

30. Pathology. 20: 73-82.

31. Hosagoudar, G.N and Chattannavar, S.N. 2008.

32. Biochemical studies in Bt and Non-Bt

33. cotton genotypes against Xanthomonasa

34. xanopodi spv. malvacearum. Journal of

35. Cotton Research and Development. 22

36. (2): 215 - 220.

37. ICAR-AICRP. 2022. Annual Report (2021-22).

38. ICAR-All India Coordinated Research

39. Project on Cotton, Coimbatore, Tamil

40. Nadu, India. pp.1-240.

41. Lalesh, K. 2020. Biochemical changes in

42. periwinkle (Catharanthus roseus) leaves

43. infected with Alternaria alternate. Journal

44. of Pharmacognosy and Phytochemistry.

45. (5): 392-394.

46. Lowry, O.H., Rosebrough, N.J., Farr, A.L and

47. Randall, R.J. 1951.Protein measurement

48. with the folin-phenol reagent. Journal of

49. Biological Chemistry.193: 265-275.

50. Malik, C.P and Singh, M.B. 1980.

51. Quantification of total phenol content. In:

52. Plant Enzymology and Histo-enzymology.

53. Kalyani publishers, New Delhi.pp. 286.

54. Mallick, S.A., Priya, K., Gupta, M and Gupta,

55. S. 2015. Effect of Alternaria blight

56. infection on biochemical parameters,

57. quantity and quality of oil of mustard

58. genotypes.Indian Journal of Plant

59. Physiology. 20 (4): 310-316.

60. Mandhania, S., Sangwan, R.S., Siwach, S.S.,

61. Pundir, S.R., Sangwan, O and Janu, O.

62. Role of biochemical constituents

63. and minerals against cotton leaf curl

64. disease in cotton. Journal of

65. Environmental Biology. 39: 221-227.

66. Miller, G.L. 1959. Use of dinitrosalicylic acid

67. reagent for determination of reducing

68. sugar. Analytical Chemistry.31 (3): 426-

69. Mukewar, P.M and Mayee, C.D. 2001. Grey

70. mildew immune cotton germplasm lines

71. BIOCHEMICAL ANALYSIS OF RESISTANT AND SUSCEPTIBLE COTTON GENOTYPES

72. registered. Indian Phytopathology. 54(1):

73. Nath, K., Solanky, K.U., Mahatma, M.K.,

74. Madhubala and Swami, R.M. 2015. Role

75. of total soluble sugars, phenols and

76. defense related enzymes in relation to

77. banana fruit rot by Lasiodiplodia

78. theobromae [(Path.) Griff. and Maubl.]

79. during ripening. Journal of Plant

80. Pathology and Microbiology. 6 (8): 299-

81. Oke, O.A. 1988. Changes in chemical

82. constituents of tobacco leaves infected

83. with Corynespora cassiicola and

84. Colletotrichum nicotianae. Journal of

85. Phytopathology.122: 181-185.

86. Pandey, M.K and Anuradha, S. 2019. Total

87. phenols and sugar content in wheat

88. resistant and susceptible cultivars

89. against karnal bunt. Plant Archives. 19

90. (1): 101-103.

91. Parakhia, A.M., Andharia, J.H and Vaishnav,

92. M.U. 1989.A new leaf spot disease of

93. cotton in India. Gujarat Agricultural

94. University Research Journal. 14 (2): 100-

95. Roshan Baba, Sk., Bhattiprolu, S.L., Prasanna

96. Kumari V and Chiranjeevi, Ch.2022.

97. Estimation of yield losses in cotton due

98. to major fungal foliar diseases. The

99. Journal of Research ANGRAU. 50 (3):

100. -27.

101. Sambroski, D.J., Forsyth, F.R and Parson, C.

102. Metabolic changes in detached

103. wheat leaves floated on benzimidazole

104. and the effect of these changes on rust

105. reactions. Canadian Journal of Botany.

106. : 591-601.

107. Mahesh, D., Bhattiprolu, S.L., Prasanna Kumari, V and Chiranjeevi, Ch. 2023. Biochemical

108. analysis of resistant and susceptible cotton genotypes against Alternaria and Corynespora.

109. The Journal of Research ANGRAU. 52 (1): 39-50

110. Sadasivam, S and Manickam, A. 1996.

111. Biochemical methods ( 2nd Edition), New

112. Age International Publishers, New Delhi,

113. India. pp.108-110.

114. Satish, S., Reeti, S and Ajay, K. 2020.The

115. biochemical changes in cluster bean

116. leaves due to Alternaria blight infection.

117. International Journal of Chemical

118. Studies. 8(3): 146-153.

119. Sidra, H., Khalid, P.A., Amjad, H., Tehsen, G.,

120. Shumaila, K., Sumaira, Y., Ghulam, A.,

121. Muhammad, J., Asghar and Nighat, S.

122. Biochemical changes in the leaves

123. of mungbean (Vigna radiata) plants

124. infected by phytoplasma. Turkish

125. Biochemical Society. 42 (6): 591-599.

126. Srivastava, N. 2018. Role of sugar compounds

127. in plant defense to Alternaria blight.

128. Indian Journal of Waste Management. 2

129. (2): 37-40.

130. Suhail, A., Khalid, K.P.A., Hameed, A.H., Ullah,

131. N., Amin, I and Abbas, G. 2020.

132. Biochemical alterations in the leaves of

133. resistant and susceptible mungbean

134. genotypes infected with mungbean

135. yellow mosaic India virus. International

136. Journal of Innovative Approaches in

137. Agricultural Research. 4(1): 99-118.

138. Sumit, K., Ram, C., Lopamudra, B., Chetan, K

139. and Estibaliz,S. 2021. Elevation of

140. systemic defense in Potato against

141. Alternaria solani by a consortium of

142. compatible Trichoderma spp. Research

143. square. pp.1-20.

144. Venkatesh, I., Bhattiprolu, S.L., Prasadji, J.K

145. and Rao, G.R. 2016. Biochemical

146. analysis of cotton genotypes infected

147. with Alternatia leaf spot disease. Journal

148. of Cotton Research and Development. 30

149. (2): 265-270.

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Published

31-03-2024

How to Cite

D. MAHESH. (2024). BIOCHEMICAL ANALYSIS OF RESISTANT AND SUSCEPTIBLE COTTON GENOTYPES AGAINST ALTERNARIA AND CORYNESPORA (S. L. BHATTIPROLU, V. PRASANNA KUMARI, & CH. CHIRANJEEVI, Trans.). The Journal of Research ANGRAU, 52(1), 39-50. https://doi.org/10.58537/jorangrau.2024.52.1.05