Association of single nucleotide polymorphism in the MBL1 gene with mastitis in Vrindavani crossbred cattle

MUHASIN ASAF V N; AMOD KUMAR; MANJIT PANIGRAHI; PRASHANT DEWANGAN; G K GAUR; BHARAT BHUSHAN

doi:10.56093/ijans.v84.i6.41656

Authors

MUHASIN ASAF V N Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
AMOD KUMAR Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
MANJIT PANIGRAHI Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
PRASHANT DEWANGAN Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
G K GAUR Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
BHARAT BHUSHAN Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v84.i6.41656

Keywords:

Allele-specific PCR, Mastitis, SNP, Somatic cell count.

Abstract

Mannose-binding lectin 1 (MBL1) is one of the most important constituent of the innate immune system. Several studies have reported that MBL1 gene polymorphisms were associated with milk somatic cell score in cattle. In the present study, the animals were grouped into mastitis negative and positive animals on the basis of California mastitis test (CMT) and somatic cell count (SCC). Genomic DNA was isolated from blood samples and allele specific PCR was used to genotype the SNP, rs110326717 (g.2651G>A) under study. The SNP was found to be polymorphic in both the affected as well as unaffected groups of cows but no significant association between the genotypes with the clinical mastitis was obtained. Although there is lack of association, further studies have to be undertaken in large population to validate the impact of rs110326717 and the host response to mastitis.

Downloads

Download data is not yet available.

References

Eisen D P and Minchinton R M. 2003. Impact of mannan-binding lectin on susceptibility to infectious diseases. The Journal of Infectious Disease 37: 1496–505.

Gjerstorff M, Hansen S, Jensen B, Dueholm B, Horn P, Bendixen C and Holmskov U. 2004. The genes encoding bovine SP-A, SP-D, MBL-A, conglutinin, CL–43 and CL–46 form a distinct collectin locus on Bos taurus chromosome 28 (BTA28) at position q.1.8–1.9. Animal Genetics 35: 333–37.

Holmskov U, Thiel S and Jensenius J C. 2003. Collectins and ficolins: humoral lectins of the innate immune defense. Annual Review of Immunology 21: 547–78.

Lillie B N, Keirstead N D, Squires E J and Hayes M A. 2006. Single nucleotide polymorphisms in porcine mannan-binding lectin A. Immunogenetics 58: 983–93.

Liu J, Ju Z, Li Q, Huang J, Li R, Li J, Ma L, Zhong J and Wang C. 2011. Mannose-binding lectin 1 haplotypes influence serum MBL-A concentration, complement activity, and milk production traits in Chinese Holstein cattle. Immunogenetics 63(11): 727–42.

Mason C. 2006. Basic mastitis bacteriology: untangling the pathogens. Irish Veterinary Journal 59: 453–59.

Schork N J, Fallin D and Lanchbury J S. 2000. Single nucleotide polymorphisms and the future of genetic epidemiology. Clinical Genetics 58(4): 250–64.

Seegers H, Fourichon C, Beaudeau F. 2003. Production effects related to mastitis and mastitis economics in dairy cattle herds. Veterinary Research 34: 475–91.

Van de Wetering J K, Golde L M and Batenburg J J. 2004. Collectins: players of the innate immune system. European Journal of Biochemistry 271: 1229–249.

Wang C, Liu M, Li Q, Ju Z, Huang J, Li J, Wang H and Zhong J. 2011. Three novel single-nucleotide polymorphisms of MBL1 gene in Chinese native cattle and their associations with milk performance traits. Veterinary Immunology and Immunopathology 139(2–4): 229–36.

Wangkumhang P, Chaichoompu K, Ngamphiw C, Ruangrit U, Chanprasert J, Assawamakin A and Tongsima S. 2007. WASP: a Web-based Allele-Specific PCR assay designing tool for detecting SNPs and mutations. BMC Genomics 8: 275.