Comprehensive study of Capripoxviruses in Moroccan small ruminants: A RT-PCR analysis

IMANE  MAHARI; SARA  GHAICHAT; HASNAE  ZEKHNINI; FATIHA  EL MELLOULI; HAMID  LAKHIARI

doi:10.56093/ijans.v94i7.147792

Authors

IMANE MAHARI Laboratory of Virology, Oncology, Biosciences, Environment and New Energies, Faculty of Sciences and Technology, BP 146, Mohammedia, Morocco
SARA GHAICHAT Laboratory of Virology, Oncology, Biosciences, Environment and New Energies, Faculty of Sciences and Technology, BP 146, Mohammedia, Morocco
HASNAE ZEKHNINI Regional Laboratory for Analysis and Research, National Office of Food Safety (ONSSA), P3003, Casablanca, Morocco
FATIHA EL MELLOULI Regional Laboratory for Analysis and Research, National Office of Food Safety (ONSSA), P3003, Casablanca, Morocco
HAMID LAKHIARI Laboratory of Virology, Oncology, Biosciences, Environment and New Energies, Faculty of Sciences and Technology, BP 146, Mohammedia, Morocco

https://doi.org/10.56093/ijans.v94i7.147792

Keywords:

Capripoxvirus, Goatpox virus, Morocco, Real time PCR, Sheeppox virus

Abstract

This study aimed to investigate and identify circulating Capripoxviruses in different regions of Morocco, using RT-PCR technique on various sample types. The results from the analysis of 980 samples lend considerable perceptions highlighting the diversity in outcomes across the tested samples. The analysis of Ct values revealed distinct patterns dependent on the nature of the sampled materials. Notably, scab samples exhibited lower Ct values indicating a higher concentration of viral load. Swab samples, in contrast, presented average Ct values signifying a moderate presence of viral DNA. Blood samples demonstrated Ct values within the range suggesting a lower viral load in the bloodstream. Furthermore, the study also revealed endemic Capripoxvirus outbreaks in Morocco, particularly in regions with a strong sheep breeding industry.

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References

Balinsky C A, Delhon G, Smoliga G, Prarat M, French R A, Geary S J and Rodriguez L L. 2008. Rapid preclinical detection of sheeppox virus by a real-time PCR assay. Journal of Clinical Microbiology 46(2): 438–42.

Gupta T, Patial V, Bali D, Angaria S, Sharma M and Chahota R. 2020. A review: Lumpy skin disease and its emergence in India. Veterinary Research Communications 44: 111–18.

Hajjou S, Boulahyaoui H, Khataby K, Loutfi C and Fakiri M. 2019. Phylogenetic analysis of Moroccan sheeppox virus isolates based on P32 gene. Revue Marocaine des Sciences Agronomiques et Vétérinaires 7(2).

Hamdi J, Bamouh Z, Jazouli M, Boumart Z, Tadlaoui K O, Fihri O F and El Harrak M. 2020. Experimental evaluation of the cross-protection between Sheeppox and bovine Lumpy skin vaccines. Scientific reports 10(1): 8888.

Hamdi J, Munyanduki H, Tadlaoui K O, Harrak M E and Fihri O F. 2021. Capripoxvirus infections in ruminants: A review. Microorganisms 9(5): 902.

Jiang C, Tao D, Geng Y, Yang H, Xu B, Chen Y and Guo A. 2022. Sensitive and specific detection of lumpy skin disease virus in cattle by CRISPR-Cas12a fluorescent assay coupled with recombinase polymerase amplification. Genes 13(5): 734.

Kumar N, Chander Y, Kumar R, Khandelwal N, Riyesh T, Chaudhary K and Tripathi B N. 2021. Isolation and characterization of lumpy skin disease virus from cattle in India. PLoS One 16(1): e0241022.

Kumar A, Gnanavel V, Hosamani M, Bhanuprakash V, Balamurugan V and Ramakrishnan M. 2022. The complete genome sequence of Indian sheeppox vaccine virus and comparative analysis with other capripoxviruses. Gene 810: 146085.

Kumar N and Tripathi B N. 2022. A serious skin virus epidemic sweeping through the Indian subcontinent is a threat to the livelihood of farmers. Virulence 13(1): 1943–44.

Lafar S, Zro K, Haegeman A, Khayli M, De Clercq K, Lancelot R and Ennaji M M. 2019. Clinical and epidemiological evolution of sheep pox in Morocco. Journal of Agricultural Science and Technology A 9: 103–13.

Mishra B, Kumar G R, Sonal S, Patel C L and Chaturvedi V K. 2018. Phylogenetic analysis of sheep pox virus (SPPV) virion core protein P4a gene revealed extensive sequence conservation among capripox viruses. The Indian Journal of Animal Sciences 88(1): 21–24.

Mishra A and Roy P. 2020. Tetra-primer amplification refractory mutation system-polymerase chain reaction (TARMS-PCR) assay in genotyping of single nucleotide polymorphism in goatpox virus p32 gene. The Indian Journal of Animal Sciences 90(2):152–155.

Ochwo S, VanderWaal K, Ndekezi C, Nkamwesiga J, Munsey A, Witto S G and Mwiine F N. 2020. Molecular detection and phylogenetic analysis of lumpy skin disease virus from outbreaks in Uganda 2017–2018. BMC Veterinary Research 16: 1–10.

ONSSA. 2017. Sheep pox in Morocco. [Online] . Retrieved from http://www.onssa.gov.ma/fr/index.php?option=com_content&view=article&id=177:clavelee-ovine&-catid=58:clavelee- ovine&Itemid=118.

Sudhakar S B, Mishra N, Kalaiyarasu S, Jhade S K, Hemadri D, Sood R, Bal G C, Nayak M K, Pradhan S K and Singh V P. 2020. Lumpy skin disease (LSD) outbreaks in cattle in Odisha state, India in August 2019: Epidemiological features and molecular studies. Transboundary and Emerging Diseases 67(6): 2408–22.

Suwankitwat N, Songkasupa T, Boonpornprasert P, Sripipattanakul P, Theerawatanasirikul S and Deemagarn T. 2022. Rapid spread and genetic characterisation of a recently emerged recombinant lumpy skin disease virus in Thailand. Veterinary Sciences 9: 542.

Tuppurainen E S M, Venter E H, Shisler J L, Gari G, Mekonnen G A, Juleff N and Babiuk L A. 2017. Capripoxvirus diseases: current status and opportunities for control. Transboundary and Emerging Diseases 64(3): 729–45.

Wen J , Yin X, Zhang X, Lan D, Liu J and Song X. 2023. Development of a real-time qpcr method for the clinical sample detection of Capripox Virus. Microorganisms 11: 2476.

WOAH. 2019. Chapter 3.07.12. Sheep pox and Goat pox. Manual of Diagnostic Tests and Vaccines.

Zewdie G, Mammo B, Gelaye E, Getachew B and Bayssa B. 2019. Isolation, molecular characterisation and vaccine effectiveness study of Lumpy Skin Disease virus in selected diary farms of Central Ethiopia. Journal of Biology, Agriculture and Healthcare 9: 764–75.

Zewdie G, Derese G, Getachew B, Belay H and Akalu M. 2021. Review of sheep and goat pox disease: Current updates on epidemiology, diagnosis, prevention and control measures in Ethiopia. Animal Diseases 1: 28.

Zro K, Zakham F, Melloul M, El Fahime E and Ennaji M M. 2014. A sheeppox outbreak in Morocco: Isolation and identification of virus responsible for the new clinical form of disease. BMC Veterinary Research 10: 1–8.