Bacterial presence and fertility in subclinical endometritic buffaloes at oestrus

HARPREET SINGH; PARKASH SINGH BRAR; A ARORA; S S DHINDSA; M HONPARKHE

doi:10.56093/ijans.v88i4.78801

Authors

HARPREET SINGH PhD Scholar, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 012 India
PARKASH SINGH BRAR Professor, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 012 India
A ARORA Professor, Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 012 India
S S DHINDSA Assistant Animal Scientist (Reproduction), Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 012 India
M HONPARKHE Gynaecologist, Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 012 India

https://doi.org/10.56093/ijans.v88i4.78801

Keywords:

Antibiotics, Bacteria, Buffaloes, Fertility, Oestrus, Subclinical endometritis

Abstract

Study evaluated the diagnosis of subclinical endometritis (SCE) at the time of oestrus in relation to uterine bacterial contamination and fertility in buffaloes (Bubalus bubalis). Normal cyclic healthy buffaloes (115) presented for insemination at spontaneous oestrus were diagnosed (by endometrial cytology) for SCE and divided into control (<5% PMN) and SCE (≥5% PMNs) groups. The prevalence of 22.61% for SCE was recorded. Significantly higher proportion of buffaloes were infected in SCE (65.4 vs 21.3%) than in control group. The seven different bacteria (Staphylococcus spp., Bacillus spp., Proteus spp., Escherichia coli, Klebsiella spp., Streptococcus spp. and Pseudomonas spp.) in both the groups with T. pyogenes only in SCE group were isolated. The presence of SCE as well as bacteria recorded a detrimental effect on fertility, with significantly lower conception rate (23.08 vs 37.08%) in SCE and tendency for higher number of services per conception in buffaloes with infected uteri, compared to their healthy counterparts. A differential trend for antibiotic sensitivity to the bacterial isolates was recorded.

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References

Arora A K, Singh J, Pangaonkar G R and Nanda A S. 2000. Bacteriological studies on the genital tract in repeat breeder bovines. International Journal of Animal Sciences 15: 205– 07.

Ata A, Türütoðlu H, Kale M, Gülay M S and Pehlivanoðlu F. 2010. Microbial flora of normal and abnormal cervical mucous discharge associated with reproductive performance of cows and heifers in estrus. Asian Australasian Journal of Animal Sciences 23(8): 1007–12. DOI: https://doi.org/10.5713/ajas.2010.90645

Azawi O I, Omran S N and Hadad J J. 2007. Clinical, bacteriological, and histopathological study of toxic puerperal metritis in Iraqi buffalo. Journal of Dairy Science 90: 4654– 60. DOI: https://doi.org/10.3168/jds.2007-0114

Azawi O I. 2010. Uterine infection in buffalo cows; A review. Buffalo Bulletin 29(3): 154–71.

Baranski W, Podhalicz-Dzie gielewska M, Zdunczyk S and Janowski T. 2012. The diagnosis and prevalence of subclinical endometritis in cows evaluated by different cytological thresholds. Theriogenology 78: 1939–47. DOI: https://doi.org/10.1016/j.theriogenology.2012.07.018

Barlund C S, Carruthers T D, Waldner C L and Palmer C W. 2008. A comparison of diagnostic techniques for postpartum endometritis in dairy cattle. Theriogenology 69: 714–23. DOI: https://doi.org/10.1016/j.theriogenology.2007.12.005

Barrio M, Marcos V, Luis A, Quintela J, Becerra J, Pedro J, García H, Daniel M B, Francisco I, Fernandez S, Alberto P, Juan C and Ana I P. 2015. Influence of subclinical endometritis on the reproductive performance of dairy cows. Spanish Journal of Agricultural Research 13(4): e05SC02. DOI: https://doi.org/10.5424/sjar/2015134-8129

Bondurant R H. 1999. Inflammation in the bovine female reproductive tract. Journal of Animal Science 77: 101–10. DOI: https://doi.org/10.2527/1999.77suppl_2101x

Brodzki P, Krzysztof N, Krzysztof K, Adam B, £ukasz K and Mrczuk J. 2014. Cytological evaluation of inflammation of the uterus and influence of endometritis on selected reproductive parameters in dairy cows. Bulletin of Veterinary Institute of Pulawy 58: 235–42. DOI: https://doi.org/10.2478/bvip-2014-0035

Clinical and Laboratory Standard Institute (CLSI). 2004. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. Informational Supplement. NCCLS Document, M31- S1,Wayne, Pennsylvania, USA.

Dar S H, Chaudhary G R, Mehrotra S, Qureshi S D, Sankar M, Jan M H, Kumar H, Chaudhary R K, Palanivelu M and Narayanan K. 2015. Association of Escherichia coli and Fusobacterium necrophorum with subclinical endometritis in postpartum Murrah buffalo. Indian Journal of Veterinary Pathology 39(4): 311–15. DOI: https://doi.org/10.5958/0973-970X.2015.00076.0

Dubuc J, Duffield T F, Leslie K E, Walton J S and LeBlanc S J. 2010. Risk factors for postpartum uterine diseases in dairy cows. Journal of Dairy Science 93: 5764–71. DOI: https://doi.org/10.3168/jds.2010-3429

Dubuc J, Duffield T F, Leslie K E, Walton J S and Leblanc S J. 2011. Randomized clinical trial of antibiotic and prostaglandin treatments for uterine health and reproductive performance in dairy cows. Journal of Dairy Science 94: 1325–38. DOI: https://doi.org/10.3168/jds.2010-3757

Ellenberger C, Rodenbusch S, Hauffe C, Lenz M, Kießling A and Sobiraj A. 2006. Subclinical bovine fertility disorders – What contribution can pathology contribute? Subclinical Fertility Disorders in Cattle. Leipzig, pp. 28–36.

Foldi J, Kulcs A R, P´ecsi A, Huyghe B, de S, Lohuisb C M, Coxb P and Huszenicza G. 2006. Bacterial complications of postpartum uterine involution in cattle. Animal Reproduction Science 96: 265–81. DOI: https://doi.org/10.1016/j.anireprosci.2006.08.006

Gahlot S C, Kumar S, Kumaresan A, Chand S, Baithalu R K, Lathika S, Patbandha T K, Lathwal S S and Mohanty T K. 2016. Efficiency of uterine fluid cytology in the diagnosis of subclinical endometritis in the water buffalo (Bubalus bubalis). Reproduction in Domestic Animals 52(3): 512–16. DOI: https://doi.org/10.1111/rda.12899

Galvão K N, Santos N R, Galvãoa J S and Gilbert R O. 2011. Association between endometritis and endometrial cytokine expression in postpartum Holstein cows. Theriogenology 76(2): 290–99. DOI: https://doi.org/10.1016/j.theriogenology.2011.02.006

Ghasemi F, Gonzalez-Cano P, Griebel P J and Palmer C. 2012. Proinflammatory cytokine gene expression in endometrial cytobrush samples harvested from cows with and without subclinical endometritis. Theriogenology 78: 1538–47. DOI: https://doi.org/10.1016/j.theriogenology.2012.06.022

Ingale A M, Rai R B, Saminathan M, Vadhana P, Hingade S S, Dhama K, Milton A A P and Singh R. 2016. Isolation, PCR detection, pathotyping and antibiogram profiling of Escherichia coli associated with endometritis in buffaloes. Journal of Animal and Plant Sciences 26(5): 1247–54.

Kasimanickam R, Duffield T F, Foster R A, Gartley C J, Leslie K E, Walton J S and Johnson W H. 2004. Endometrial cytology and ultrasonography for the detection of subclinical endometritis in postpartum dairy cows. Theriogenology 62: 9–23. DOI: https://doi.org/10.1016/j.theriogenology.2003.03.001

Madoz L, Giuliodori M, Jaureguiberry M, Plöntzke J, Drillich M and De la Sota R. 2013. The relationship between endometrial cytology during estrous cycle and cutoff points for the diagnosis of subclinical endometritis in grazing dairy cows. Journal of Dairy Science 96: 4333–39. DOI: https://doi.org/10.3168/jds.2012-6269

Madoz L V, Giuliodori M J, Migliorisi A L, Jaureguiberry M and De la Sota R. 2014. Endometrial cytology, biopsy, and bacteriology for the diagnosis of subclinical endometritis in grazing dairy cows. Journal of Dairy Science 97: 195–201. DOI: https://doi.org/10.3168/jds.2013-6836

McDougall S, Hussein H, Aberdein D, Buckle K, Roche J, Burke C, Mitchell M and Meier S. 2011. Relationships between cytology, bacteriology and vaginal discharge scores and reproductive performance in dairy cattle. Theriogenology 76: 229–40. DOI: https://doi.org/10.1016/j.theriogenology.2010.12.024

Quinn P J, Carter M E, Markey B and Carter G R. 1999. Bacterial pathogen, microscopy culture and identification. Text Book of Clinical Veterinary Microbiology. Mosby Publication, New York.

Ricci A, Gallo S, Molinaro F, Dondo A, Zoppi S and Vincenti L. 2015. Evaluation of subclinical endometritis and consequences on fertility in piedmontese beef cows. Reproduction in Domestic Animals 50: 142–48. DOI: https://doi.org/10.1111/rda.12465

Samatha V and Ramesh B K. 2013. Bacteriological and cytological studies of endometritis in buffaloes. International Journal of Food, Agriculture and Veterinary Sciences 3(2): 6–9.

Sayyari M, Farahangnia M, Ghaemmaghami S H and Sharma R H. 2012. A comparative study on bacteriology and pathology in uteri of cattle and buffalo in Ahwaz region, Iran. Iranian Journal of Veterinary Medicine 6(1): 32–39.

Seh B A, Wani S A, Khan M Z and Wani G M. 2000. In vitro sensitivity pattern of bacteria isolated from uterine secretions of repeat breeder cows. Indian Journal of Animal Reproduction 21: 61–62.

Sheldon I M and Dobson H. 2004. Postpartum uterine health in cattle. Animal Reproduction Science 82–83: 295–306. DOI: https://doi.org/10.1016/j.anireprosci.2004.04.006

Shukla S P and Sharma R D. 2005. Bacteriological studies on the uterine biopsy and conception rate following treatment in repeat breeder crossbred cows. Indian Journal of Animal Reproduction 26: 17–19.

Singh J, Murray R D, Mshelia G and Woldehiwet Z. 2008. The immune status of the bovine uterus during the peripartum period. Veterinary Journal 175: 301–09. DOI: https://doi.org/10.1016/j.tvjl.2007.02.003

Udhayavel S, Malmarugan S, Palanisamy K and Rajeswar J. 2013. Antibiogram pattern of bacteria causing endometritis in cows. Veterinary World 6(2): 100–102. DOI: https://doi.org/10.5455/vetworld.2013.100-102

Wagener K, Christoph G and Drillich M. 2017. A review of the ongoing discussion about definition, diagnosis and pathomechanism of subclinical endometritis in dairy cows. Theriogenology 94: 21–30. DOI: https://doi.org/10.1016/j.theriogenology.2017.02.005

Werner A, Suthar V, Plöntzke J and Heuwieser W. 2012. Relationship between bacteriological findings in the second and fourth weeks postpartum and uterine infection in dairy cows considering bacteriological results. Journal of Dairy Science 95: 7105–14. DOI: https://doi.org/10.3168/jds.2012-5627