Phosphorus, protein and DNA contents of buffalo oocytes before and after in vitro maturation: effects of IGF-I


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Authors

  • S Nandi National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore, Karnataka
  • P S Gupta National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore, Karnataka
  • J P Ravindra National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore, Karnataka

Keywords:

Buffalo, DNA, IGF-I, IVM, Oocytes, Phosphorus, Protein

Abstract

Studies were undertaken to determine the phosphorus, protein and DNA contents of viable buffalo oocytes before and after in vitro maturation with supplementation of different concentrations of IGF-I (0, 25, 100, 200 ng/ml) in culture medium. Oocytes were aspirated from abattoir derived buffalo ovaries. The oocytes with homogenous ooplasm and compact cumulus cell layers were preliminarily selected and stained with trypan blue to test their vitality. The concentration of 200 ng/ml of IGF-I in the maturation medium was optimum for in vitro maturation of buffalo oocytes. TGF-I stimulated in vitro oocyte maturation in a dose-dependent manner. The phosphorus, protein and DNA were significantly higher in oocytes after culture compared to those before culture in all treatments.

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Submitted

2011-09-15

Published

2007-09-05

Issue

Vol. 77 No. 9 (2007)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

Nandi, S., Gupta, P. S., & Ravindra, J. P. (2007). Phosphorus, protein and DNA contents of buffalo oocytes before and after in vitro maturation: effects of IGF-I. The Indian Journal of Animal Sciences, 77(9). https://epubs.icar.org.in/index.php/IJAnS/article/view/10253