Outer membrane protein profiles of Pasteurella multocida isolates of bovine origin


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Authors

  • A K Arora Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab
  • S K Jand Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab
  • K S Sandhu Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab
  • M S Oberoi Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab

Keywords:

Bovine, Haemorrhagic septicaemia, Immunoblotting, Outer membrane proteins, Pasteurella multocida

Abstract

Outer membrane protein (OMP) profiles of 17 different Pasteurella multocida isolates of bovine origin were studied by SDS-PAGE and immunoblotting. The proteins were extracted by sonication, ultra centrifugation and detergent (sodium lauroyl sarcosinate) treatment. All the isolates showed homogenous protein profiles comprising 23 different polypeptide bands ranging in molecular weight from 13 to 94 kDa. On the basis of stain intensity 32 kDa protein appeared to be the major protein band followed by 25 and 28 kDa bands. Other protein bands observed were 13,34, 44.5,46, 80 and 84 kDa. Immunoblotting was carried out using homologous antiserum raised in rabbits after single injection of formalin-killed antigen. The 32 kDa protein was found to be the immunodominant along with 2.5 kDa protein band in all the isolates.

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Submitted

2011-09-16

Published

2007-08-05

Issue

Vol. 77 No. 8 (2007)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

Arora, A. K., Jand, S. K., Sandhu, K. S., & Oberoi, M. S. (2007). Outer membrane protein profiles of Pasteurella multocida isolates of bovine origin. The Indian Journal of Animal Sciences, 77(8). https://epubs.icar.org.in/index.php/IJAnS/article/view/10357