Characterization of growth hormone releasing hormone (GHRH) partial gene in buffalo


161 / 31

Authors

  • Rajamurugan J Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh
  • Subodh Kumar Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh
  • O P Pandey Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh
  • S M Deb Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh
  • Abhijit Mitra Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh
  • Arjava Sharma Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh

Keywords:

uffalo, Bubalus bubalis, Growth hormone releasing hormone, PCR-RFLP

Abstract

A fragment of 453 bp corresponding to exon 2 and 3 of growth hormone releasing hormone (GHRH) gene was amplified in 60 buffaloes (Bubalus bubalis) of Murrah breed. The HaeIII PCR-RFLP analysis showed the absence of mutant allele and hence monomorphism in the buffalo population studied. Sequence homology indicated that the buffalo GHRH was closest to that of yak with 96.2% similarity, then followed by cattle (95.8%), horse (86.4%), pig (72%), dog (48.5%), rat (32.1%) and mouse (29.4%). The amino acid homology between buffalo and cattle was 100% despite some nucleotide changes. The sequence of the amplicon, which was the first report on buffalo GHRH, was submitted to GenBank.

Downloads

Download data is not yet available.

Downloads

Submitted

2011-09-16

Published

2007-08-05

Issue

Vol. 77 No. 8 (2007)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

J, R., Kumar, S., Pandey, O. P., Deb, S. M., Mitra, A., & Sharma, A. (2007). Characterization of growth hormone releasing hormone (GHRH) partial gene in buffalo. The Indian Journal of Animal Sciences, 77(8). https://epubs.icar.org.in/index.php/IJAnS/article/view/10369