Development of primary culture from heart tissue of Chitala chitala (Hamilton-Buchanan)
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Authors
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SMITA KAPOOR
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KAMALENDRA KAMALENDRA
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BHATT J P
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LAKRA W S
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RATHORE G
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GOSWAMI M
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T RAJA SWAMINATHAN
Keywords:
Chital, Heart, Primary cultureAbstract
A method for developing cell cultures from Chitala chitala heart tissue is described. The tissues were minced and seeded in culture flasks and grown in Leibovitz L-15 medium supplemented with 20% foetal bovine serum. The radiation started after 4th day in heart. The medium was changed after every five days. The radiations were able to withstand a wide range of temperatures from 25 °C to 28°C with an optimum temperature of 28°C. A partial monolayer was observed after 12 days in heart tissue. A primary culture was successfully obtained from heart tissue of C. chitala for the first time in India.
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