Development of primary culture from heart tissue of Chitala chitala (Hamilton-Buchanan)


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Authors

  • SMITA KAPOOR
  • KAMALENDRA KAMALENDRA
  • BHATT J P
  • LAKRA W S
  • RATHORE G
  • GOSWAMI M
  • T RAJA SWAMINATHAN

Keywords:

Chital, Heart, Primary culture

Abstract

A method for developing cell cultures from Chitala chitala heart tissue is described. The tissues were minced and seeded in culture flasks and grown in Leibovitz L-15 medium supplemented with 20% foetal bovine serum. The radiation started after 4th day in heart. The medium was changed after every five days. The radiations were able to withstand a wide range of temperatures from 25 °C to 28°C with an optimum temperature of 28°C. A partial monolayer was observed after 12 days in heart tissue. A primary culture was successfully obtained from heart tissue of C. chitala for the first time in India.

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Issue

Vol. 82 No. 1 (2012)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

KAPOOR, S., KAMALENDRA, K., P, B. J., S, L. W., G, R., M, G., & SWAMINATHAN, T. R. (2012). Development of primary culture from heart tissue of Chitala chitala (Hamilton-Buchanan). The Indian Journal of Animal Sciences, 82(1). https://epubs.icar.org.in/index.php/IJAnS/article/view/13869