Differential muscle methylome analysis in Mali and Hampshire pig breeds

PRAJWALITA  PATHAK; N H  MOHAN; JURI  DEKA; JAYA  BHARATI; A K  DAS; R  THOMAS; R  SINGH; V K  GUPTA; B C  DAS

doi:10.56093/ijans.v96i3.143165

Authors

PRAJWALITA PATHAK Krishi Vigyan Kendra, Dhemaji, Simen Chapori 787 061, Assam, India
N H MOHAN ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana 132 001, India
JURI DEKA Krishi Vigyan Kendra, Dhemaji, Simen Chapori 787 061, Assam, India
JAYA BHARATI Krishi Vigyan Kendra, Dhemaji, Simen Chapori 787 061, Assam, India
A K DAS Krishi Vigyan Kendra, Dhemaji, Simen Chapori 787 061, Assam, India
R THOMAS Krishi Vigyan Kendra, Dhemaji, Simen Chapori 787 061, Assam, India
R SINGH Krishi Vigyan Kendra, Dhemaji, Simen Chapori 787 061, Assam, India
V K GUPTA Krishi Vigyan Kendra, Dhemaji, Simen Chapori 787 061, Assam, India
B C DAS Krishi Vigyan Kendra, Dhemaji, Simen Chapori 787 061, Assam, India

https://doi.org/10.56093/ijans.v96i3.143165

Keywords:

DNA methylation, Muscle, Myogenesis, Pig

Abstract

Muscle development and carcass yield of meat indigenous and exotic pigs vary significantly. DNA methylation, being a major epigenetic factor, could regulate myogenic process through regulation of gene expression. The objective of the present study was to compare genome-wide methylation in indigenous (Mali) and exotic (Hampshire) breeds of pigs. A total 1549 differentially methylated regions (P<0.1) in the genome with hypermethylation around the promoter-transcription start site of MYMK gene were identified in Mali pigs. No differences in the DNA methylation was observed with respect to promoter region of other myogenic genes. Ontology of genes whose bodies were differentially methylated, were related to gaseous exchange, lipid metabolism, opioid, nephrin and DARPP-32 signalling. Based on the study, it may be concluded that differences exist in the muscle tissue DNA methylation between Mali and Hampshire pigs. Further studies are required to understand the implication of the differential methylation in myogenic process in pigs.

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