Differential muscle methylome analysis in Mali and Hampshire pig breeds
Differential methylation in porcine muscle
3
Authors
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Prajwalita Pathak
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Narayana H. Mohan
Principal Scientist
ICAR-National Research Centre on Pig
Rani, Guwahati 781131.
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Juri Deka
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Jaya Bharati
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Anil Kumar Das
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R Thomas
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R Singh
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Vivek Kumar Gupta
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Bikash Chandra Das
Keywords:
Pig, muscle, DNA, Methylation, myogenesisAbstract
The muscle development and hence the carcass yield of meat indigenous and exotic pigs vary significantly. DNA methylation being a major epigenetic factor could regulate myogenic process through regulation of gene expression. The objective of the study was to compare genome-wide methylation in indigenous (Mali) and exotic (Hampshire) breeds of pigs. We identified 1549 differentially methylated regions (P<0.1) in the genome with hypermethylation around the promoter-transcription start site of MYMK gene in Mali pigs. No differences in the DNA methylation was observed with respect to promoter region of other myogenic genes. Ontology of genes whose bodies were differentially methylated, were related to gaseous exchange, lipid metabolism, opioid, nephrin and DARPP-32 signalling. Based on the study, we conclude that there are differences in the muscle tissue DNA methylation between Mali and Hampshire pigs. Further studies are required to understand the implication of the differential methylation in myogenic process in pigs.
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