Protective effect of Mangifera indica Linn. on gentamicin induced nephrotoxicity in rats Nephroprotective effect of stem bark of Mangifera indica (M. indica) against gentamicin-induced nephrotoxicity in rats

Abstract

Nephroprotective effect of stem bark of Mangifera indica (M. indica) against gentamicin-induced nephrotoxicity in male Sprague Dawley rats was assessed. Rats administered with gentamicin daily @ 80 mg/kg i/p, for 8 days showed an increase in lipid peroxidation and a decrease in superoxide dismutase, catalase and reduced glutathione. Significant (P<0.05) increase in serum creatinine and urea levels was also observed. Histopathological examination of kidney revealed extensive proximal tubular necrosis with marked glomerular changes. Ethanolic extract of M indica at a dose rate of 100 mg/kg p.o. and 500 mg/kg p.o. showed a significant (P<0.05) reduction in lipid peroxidation and an increase in the activities of superoxide dismutase, catalase and reduced glutathione, which suggest its efficacy in scavenging free radical-induced renal damage. The ethanolic herbal extract also showed a reduction in serum creatinine and serum urea levels. These effects were predominant with 500 mg/kg p.o. Histopathological examination of tissue sections revealed tubular vacuolar degeneration and necrosis in a dose dependent manner. Thus, the study revealed that treatment with ethanolic extract M indica inhibited gentamicin-induced proximal tubular necrosis though the dose rate was not sufficient to provide complete protection.