Impact of Incremental Zinc Sulphate Monohydrate on Productive Performance, Blood Chemistry and Egg Quality Dynamics of Bovan Black Strain
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Keywords:
feed efficiency, hen-day production, immunoglobulin, mortality, pulletsAbstract
This study evaluated the effect of zinc sulphate monohydrate on performance characteristics and blood chemistry of laying birds. A total of 100 Point of Lay (PoL) Bovan Black hens were randomly assigned to four dietary treatments: control (T1, 0mg/kg zinc), and treatments T2 (30mg/kg), T3 (60mg/MT), and T4 (90mg/kg). Birds were allowed access to feed and water ad libitum for 12 weeks. Parameters assessed included: feed proximate composition (%), Hen-Day Production (HDP), feed intake, egg weight, feed efficiency, percentage mortality, and blood biochemical indices. Dry matter ranged from 85.07% (T1) to 82.39% (T4), as crude protein slightly increased from 16.68.58% (T1) to 16.94% (T4). While zinc levels peaked in T4 (22.59 mg/kg), calcium (1.17%) and phosphorus (0.64–0.65%) remained constant across treatments. HDP was significantly (p<0.05) higher in T3 (70.13%) than T4 (62.52%) as Feed efficiency improved in T2 (3.17%). Total Antioxidant Capacity was significantly higher (p<0.05) in T2 (14.34 mM) than T1 (7.37 mM), as T3 (9.79 mM) and T4 (11.72 mM) showed intermediate values. Glutathione peroxidase activity was also significantly higher (p<0.05) in T2 (36.01 U/L) compared to T1 (25.13 U/L), T3 (28.88 U/L), and T4 (24.38 U/L), percentage mortality and egg quality were unaffected. Moderate zinc supplementation (30-60mg/kg) enhanced laying performance and oxidative status without adverse effects. Therefore, incorporation of zinc in laying hen’s diets enhances growth performance, improved egg quality parameters, and positively influenced health indicators and antioxidant status, highlighting its potential role in optimizing poultry nutrition and enhancing health management practices.
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1. Abedini M, Shariatmadari F, Karimi M A and Ahmadi H. 2018. Effects of zinc oxide nanoparticles on the egg quality, immune response, zinc retention, and blood parameters of laying hens in the late production phase. Journal of Animal Physiology and Animal Nutrition 102 (3): 736–745.
2. Abou-Kassem D E, Ahmed H E and Attia Y A. 2020. Impact of organic versus inorganic zinc supplementation on laying hens’ performance and egg quality. Animal Production Science 60 (3): 395–403.
3. Aguihe P C, Kehinde A S, Ntagbu F G et al. 2024. Response of broiler finishers to mixture of sun-dried cassava and sweet potato peel meal based diets with acidifier supplementation. Nigerian Journal of Animal Production 51 (2): 1026–1029.
4. Ali M, Zhang L and Li W. 2021. Zinc supplementation in poultry nutrition: Benefits and risks. Animal Nutrition 7 (3): 580–591.
5. AOAC. 1990. Association of Official Method of Analysis, 18th Edition. Association of Analytic Chemists, Washington D.C., U.S.A.
6. Attia Y A, Abd Al-Hamid A E, Zeweil H S et al. 2013. Effect of dietary amounts of inorganic and organic zinc on productive and physiological traits of White Pekin ducks. Animal 7 (6): 895–900.
7. Aya A M, Elsayed S S, Abol-Ela A A, Askar et al. 2021. Supplementation of different zinc sources to low-CP diets and its effect on performance, carcass traits, liver and kidney functions, immunological, and antioxidant parameters of quail chicks. Poultry Science 100: 101463.
8. Bao Y, Choct M, Iji P and Bruerton K. 2007. Effect of organically complexed cuprum, iron, manganese, and zinc on broiler performance, mineral excretion, and accumulation in tissues. Journal of Applied Poultry Research 16 (3): 448–455.
9. Batal A B, Parr T M and Baker D H. 2001. Zinc bioavailability in tetrabasic zinc chloride and the dietary zinc requirement of young chicks fed a soy concentrate diet. Poultry Science 80 (1): 87–90.
10. Chen X, He C, Zhang K, Wang J, Ding X et al. 2022. Comparison of zinc bioavailability in zinc-glycine and zinc-methionine chelates for broilers fed with a corn-soybean meal diet. Frontiers in Physiology 13: 983954.
11. Debbarma R K, Ali M A., Lallianchhunga M C., Das H, Kalita G et al. 2023. Vitamin C supplementation ameliorates summer stress and improves antioxidative status of commercial layer reared in Mizoram. The Indian Journal of Animal Sciences, 93(6), 649–654.
12. De Grande A, Leleu S, Delezie E, Rapp C et al. 2020. Dietary zinc source impacts intestinal morphology and oxidative stress in young broilers. Poultry Science 99 (1): 441–453.
13. Elnesr S S, Mahmoud B Y, da Silva Pires P G et al. 2024. Trace minerals in laying hen diets and their effects on egg quality. Biological Trace Element Research 202: 5664–5679.
14. Hidayat C, Sumiati, Jayanegara A, Wina E. 2020. Effect of zinc addition on the immune response and production performance of broilers: a meta-analysis. Asian-Australasian Journal of Animal Sciences 33 (3): 465–479.
15. Huang L, Li X, Wang W, Yang L and Zhu Y. 2019. The role of zinc in poultry breeder and hen nutrition: an update. Biological Trace Element Research 192 (2): 308–318.
16. Hudson B P, Dozier W A, Fairchild B D, Wilson J L, Sander J E, Ward T L. 2004. Live performance and immune responses of straight-run broilers: influences of zinc source in broiler breeder hen and progeny diets and ambient temperature during the broiler production period. Journal of Applied Poultry Research 13 (2): 291–301.
17. Idahor K O, Osayande U D, Sokunbi O A, Osaiyuwu O H, Adua M M, Igoche L E, Kaye J. 2018. Reproduction performance, serum biochemical and growth indices of grower rabbits (Oryctolagus cuniculus) fed sheabutter (Vitellaria paradoxa CF Gaertn.) nut meal. International Journal of Research Studies in Zoology 4 (1): 22–28.
18. Ilaboya I I, Imouokhome J I, Osayande U D, Ibiezugbe J E, Iyayi E A. 2024. Impact of phytase and cholecalciferol supplementation on the true digestibility of phosphorus by broiler chickens fed some agro-based byproducts. Advanced Research in Biological Science 9: 141–170.
19. Jimoh O A, Oluyemi A A, Ayodele S O, Osayande U D, Olarotimi O J. 2024. Impact of scrap dumpsite leachates on African land snails: insights into toxicity, biochemical responses, and reproductive implications. Archiva Zootechnica 27 (2): 59–74. http://doi:10.2478/azibna-2024-0015
20. Jimoh O A, Osayande U D, Ayodele S O, Ihejirika U G D. 2025. Nutraceutical effects of snot apple powder on triiodothyronine, oxidative stress markers, haematology and growth of broiler chickens. Black Sea Journal of Agriculture 8 (1): 5–6.
21. khondowe P, Mutayoba B, Muhairwa A, Phiri E. 2021. Effects of heat stress and a low energy diet on blood parameters and liver hsp70 and iNOS gene expressions in local chickens. Veterinary and Animal Science 14: 100221.
22. Li L, Li H, Zhou W, Feng J, Zou X. 2019. Effects of zinc methionine supplementation on laying performance, zinc status, intestinal morphology, and expressions of zinc transporters’ mRNA in laying hens. Journal of the Science of Food and Agriculture 99 (14): 6582–6588.
23. Min Y N, Liu F X, Qi X, Ji S, Cui L, Wang Z P, Gao Y P. 2019. Effects of organic zinc on tibia quality, mineral deposit, and metallothionein expression level of aged hens. Poultry Science 98 (1): 366–372.
24. Niknia A D, Vakili R, Tahmasbi A M. 2022. Zinc supplementation improves antioxidant status, and organic zinc is more efficient than inorganic zinc in improving the bone strength of aged laying hens. Veterinary Medicine and Science 8 (5): 2040–2049.
25. Niu Z, Liu F, Sun Y. 2021. Role of zinc in reproductive health and fertility in poultry. Reproductive Biology 21 (4): 277–284.
26. NRC. 1994. Nutrient Requirements of Poultry, 9th Revised Edition. National Research Council, National Academy Press, Washington D.C.
27. Obajuluwa O V, Sanwo K A, Egbeyale L T, Fafiolu A O, Osayande U D, Eguaoje A S. 2024. Nutrient digestibility of broiler chickens fed larvacide and yohimbe bark powder supplemented diets. Nigerian Journal of Animal Production 51 (2): 896–899.
28. Ogbuewu I P, Mbajiorgu C A. 2022. Meta-analysis of zinc supplementation on laying performance, egg quality characteristics, and blood zinc concentrations in laying hens. Biological Trace Element Research 200 (12): 5188–5204
29. Osayande U D, Ilaboya I I. 2024. Utilization of cottonseed meal with or without phytase supplementation on performance and haematological parameters during short term feeding periods in broiler chickens. Fudma Journal of Sciences 8 (3): 184–189.
30. Qi X, Ma S, Liu X, Wang Y, Liu Y, Gao Y, Min Y. 2019. Effects of the methionine hydroxyl analogue chelate zinc on antioxidant capacity and liver metabolism using H-1-NMR-based metabolomics in aged laying hens. Animals 9 (11): 898.
31. Rao S V R, Prakash B, Kumari K et al. 2013. Effect of supplementing different concentrations of organic trace minerals on performance, antioxidant activity, and bone mineralization in Vanaraja chickens developed for free range farming. Tropical Animal Health and Production 45 (6): 1447–1451.
32. Rath N C, Anthony N B, Huff G R. 2022. Zinc deficiency and immune function in poultry: a review. Poultry Science 101 (4): 1352–1365.
33. Saleh A A, Eid Y Z, Ebeid T A. 2022. Effect of zinc supplementation on feed efficiency and egg production in laying hens. Veterinary World 15 (1): 53–60.
34. Saleh A A, Eltantawy M S, Gawish E M, Younis H H, Amber K A, Abd El-Moneim A E E, Ebeid T A. 2020. Impact of dietary organic mineral supplementation on reproductive performance, egg quality characteristics, lipid oxidation, ovarian follicular development, and immune response in laying hens under high ambient temperature. Biological Trace Element Research 195 (2): 506–514.
35. Shao Y, Li X, Du S, Sun X, Wang Y, Zhao D, Wang Z. 2023. Effect of dietary supplemental zinc on laying performance, egg quality, and plasma hormone levels of breeding pigeons. Biological Trace Element Research 201 (6): 2991–2999. https pasakite
36. Statistical software (SAS). 2002. Statistical Analysis System, Statistical methods. SAS Institute Inc., Cary, N.C.
37. Wang Z C, Yu H M, Xie J J, Cui H, Nie H, Zhang T, Gao X H. 2019. Effect of dietary zinc pectin oligosaccharides chelate on growth performance, enzyme activities, Zn accumulation, metallothionein concentration, and gene expression of Zn transporters in broiler chickens. Journal of Animal Science 97 (5): 2114–2124.
38. Yu Q, Liu H, Yang K, Tang X, Chen S, Ajuwon K M, Degen A, Fang R. 2020. Effect of the level and source of supplementary dietary zinc on egg production, quality, and zinc content and on serum antioxidant parameters and zinc concentration in laying hens. Poultry Science 99 (11): 6233–6238.
39. Zaghari M, Mehrvarz H, Hajati H, Moravej H. 2022. Evaluation of an innovative Zn source on feed efficiency, growth performance, skin and bone quality of broilers suffering heat stress. Animals 12 (23): 3272.
40. Zhang J, Li Z, Yu C, Liu H, Zhou B, Zhang X, Wang T, Wang C. 2022. Efficacy of using zinc oxide nanoparticle as a substitute to antibiotic growth promoter and zinc sulphate for growth performance, antioxidant capacity, immunity and intestinal barrier function in broilers. Italian Journal of Animal Science 21 (1): 562–576.
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