EFFECT OF DIETARY SUPPLEMENTATION OF ALOE VERA (Aloe barbadensis) AND TULSI (Ocimum sanctum) POWDER ON GROWTH PERFORMANCE OF BROILER CHICKENS
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Keywords:
Aloe barbadensis , Broiler chicken, Growth performance, Ocimum sanctumAbstract
A feeding trial was conducted to assess the impact of dietary supplementation with Aloe barbadensis (Aloe vera), Ocimum sanctum (Tulsi) powder, and their combination on broiler performance over 35 days. A total of 200-day-old Cobb 400Y broiler chicks were procured, individually weighed, and randomly distributed into four treatment groups (T0–T3) of 50 birds each, with five replicates of 10 birds per group. The diets were iso-nutritive and included: T0 (basal diet, control), T1 (basal diet + 1.5% Aloe vera), T2 (basal diet + 1% Tulsi), and T3 (basal diet + 1.5% Aloe vera + 1% Tulsi). Results demonstrated that broilers in the T3 group (Aloe vera + Tulsi) showed significant (P<0.05) improvements in feed intake, weekly weight gain, feed conversion ratio (FCR), and performance index compared to the control. Performance ranking was T3 > T2 > T1 > T0. Additionally, retention of dry matter, crude protein, and ether extract was significantly enhanced in supplemented groups, with the highest values in T3. However, calcium and phosphorus retention remained statistically similar across treatments. In summary, combined supplementation of Aloe vera and Tulsi powder effectively improved growth performance, nutrient utilization, and feed efficiency, highlighting its potential as a natural growth promoter in broiler production.
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Akhtar M, Hai A, Awais M M, Iqbal Z, Muhammad F and Anwar M I. 2012. Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens. Veterinary Parasitology 186: 170-177.
Alemi I F, Mahdavi A, Ghazvinian K, Ghader M and Darabighane B. 2012. The effects of different levels of Aloe vera gel powder on antibody titer against Newcastle disease virus and performance in broilers. Proceedings of International Poultry Scientific Forum, Georgia World Congress Center, Atlanta, Georgia, 47: 23-26.
AOAC. 2005. Official method of Analysis. 18th Edition, Association of Officiating Analytical Chemists, Washington DC, Method 935.14 and 992.24.
Appusamy J. 2012. Effect of supplementation of Aloe Vera extracts on growth performance in commercial broilers. The Indian journal of Field Veterinarians 8(1): 47-49.
Bhosale D S, Bhagwat S R, Pawar M M and Kulkarni R C. 2015. Comparative efficacy of dietary addition of tulsi (Ocimum sanctum) leaf powder and vitamin E on broiler performance. Indian Journal of Animal Nutrition 32(3): 348-350.
BIS. 2007. Indian standard, poultry feeds specifications, IS-1374:2007. Bureau of Indian Standards. 9, Bahadur Shah Zafar Marg, Manak Bhawan, New Delhi, India.
Boudreau M D and Beland F A. 2006. An evaluation of the biological and toxicological properties of Aloe barbadensis (miller), Aloe vera. Journal of Environmental Science and Health 24(1):103-154.
Choudhary D, Kaur R, Chandrasekaran B, Rani N, Kumar B, Mannan A, Rawat R, Eyupoglu V, Marwaha M and Singh T G. 2025. Exploring phyto-resources of tulsi for the management of depression: a discovery through computational and pharmacological studies. Chemistry Africa https://doi.org/10.1007/s42250-025-01438-2
Choudhary M, Kochhar A and Sangha J. 2014. Hypoglycemic and hypolipidemic effect of Aloe vera L. in non-insulin dependent diabetics. Journal of Food Science and Technology 1(1): 90-96.
Darabighane B, Zarei A and Shahneh A Z. 2012. The effects of different levels of Aloe vera gel on ileum microflora population and immune response in broilers: a comparison to antibiotic effects. Journal of Applied Animal Research 40: 31-36.
Devi P C, Samanta A K, Das B and Kalita G. 2015. Effect of plant extracts and essential oil blend as alternatives to antibiotic growth promoters on growth performance, nutrient utilization and carcass characteristics of broiler chicken. Indian Journal of Animal Nutrition 35(4):421.
Durrani F R, Ullah S, Chand N, Durrani Z and Akhtar S. 2008. Using aqueous extract of aloe gel as anticoccidial and immunostimulant agent in broiler production. Sarhad Journal of Agriculture 24(4): 665-670.
El-Banna H A, El Latif A A and Soliman M. 2013. Anticoccidial Activity of Allium sativum and Aloe Vera in Broiler Chickens. International Journal for Agro Veterinary and Medical Sciences 7(4): 117-125.
Hasan M N, Mostofa M, Sorwar M G, Hasan M T, Das K and Hossain D M N. 2016. Effects of Tulsi leaf extract on body weight gain in broiler production. Bangladesh Journal of Veterinary Medicine 14(1): 21-25.
Hassanbeigy-Lakeh Z, Roustaee Ali-Mehr M and Haghighian-Roudsari M. 2012. Effect of Aloe gel on broiler performance. Proceeding of 5th Iranian Congress on Animal Science, Isfahan, Iran, pp. 973-977.
Kumar K P S, Bhowmik D, Chiranjib and Biswajit. 2010. Aloe vera: a potential herb and its medicinal importance. Journal of Chemical and Pharmaceutical Research 2(1): 21-29.
Lanjewar R D, Zanzad A A, Ramteke B N and Deshmukh G B. 2009. Effect of dietary supplementation of Tulsi (Ocimum sanctum) leaf powder on the growth performance and serum lipid profile in broilers. Indian Journal of Animal Nutrition 25(4): 395-397.
Mehala C and Moorthy M. 2008. Effect of aloe vera and Curcuma longa (turmeric) on carcass characteristics and biochemical parameters of broilers. International Journal of Poultry Science 7(9): 857-861.
Mishra S and Srivastava A. 2015. To study the physico-chemical properties of Bael and Aloe vera blended beverages. International Journal of Science and Research 4(9): 642-645.
Moorthy M, Saravanan S, Mehala C, Ravi S, Ravikumar M, Viswanathan K and Edwin S C. 2009. Performance of single comb white leghorn layers fed with aloe vera, curcuma longa (turmeric) and probiotic. International Journal of Poultry Science 8(8): 775-778.
Raziq F, Khan S, Chand N, Sultan A, Mushtaq M, Rafiullah R, Suhail S and Zeb A. 2012. Effect of water-based infusion of Aloe barbedensis, Pimpinella anisum, Berberis lycium, Trigonella foenum-graecum and Allium sativum on the performance of broiler chicks. Pakistan Veterinary Journal 32: 593-596.
Singh V, Amdekar S and Verma O. 2010. Ocimum sanctum (Tulsi): Bio-pharmacological Activities. Web med Central Pharmacology. 1(10): 1046.
Snedecor G W and Cochran W G. 1994. Statistical Methods. 9th edn. The Iowa State University Press, Ames, Iowa.
SPSS. 2011. Statistical Packages for Social Sciences Version 20.0. SPSS, Chicago, IL, USA.
Srivastava S B, Niwas R, Singh D P and Bisen B R. 2013. Impact of herbal based diets on production efficiency of broiler The Bioscan 8(1): 119-122.
Swathi B, Gupta P S P and Nagalakshmi D. 2012. Effect of tulsi (Ocimum sanctum) and turmeric (Curcuma longa) on broiler performance and blood constituents during heat stress in broilers. International Journal of Pharma and Bio Sciences 3(3): 446-453.
Talapatra S K, Roy S C and Sen K C. 1940. The analysis of mineral constituents in biological materials. I. Estimation of phosphorus, calcium, magnesium, sodium and potassium in food stuffs. Indian Journal of Veterinary Science and Animal Husbandry 10: 243-258.
Vasanthakumar P, Sasikumar P, Pangayarselvi B, Chandrasekaran D, Doraisamy K A, Senthilkumar S and Purushothaman M R. 2013. Performance of broiler chicken fed Tulsi leaf powder and leaf extract supplemented diets during summer to alleviate heat stress. The Indian Journal of Animal Sciences 83(9): 930-931.
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