Curcumin and L cysteine mitigates cryopreservation-inducedcryodamage in Barbari buck semen

JAYANTH  M N; BHOOPENDRA  SINGH; RAJESH  KUMAR; SUSHANT  SRIVASTAVA; JASWANT  SINGH; MANOJ KUMAR  VERMA

doi:10.56093/ijans.v96i3.176097

Authors

JAYANTH M N Acharya Narendra Deva University of Agriculture and Technology, Kumarganj Ayodhya 224229 Uttar Pradesh India
BHOOPENDRA SINGH Acharya Narendra Deva University of Agriculture and Technology, Kumarganj Ayodhya 224229 Uttar Pradesh India
RAJESH KUMAR Acharya Narendra Deva University of Agriculture and Technology, Kumarganj Ayodhya 224229 Uttar Pradesh India
SUSHANT SRIVASTAVA Acharya Narendra Deva University of Agriculture and Technology, Kumarganj Ayodhya 224229 Uttar Pradesh India
JASWANT SINGH Acharya Narendra Deva University of Agriculture and Technology, Kumarganj Ayodhya 224229 Uttar Pradesh India
MANOJ KUMAR VERMA Acharya Narendra Deva University of Agriculture and Technology, Kumarganj Ayodhya 224229 Uttar Pradesh India

https://doi.org/10.56093/ijans.v96i3.176097

Keywords:

Barbari Buck, Cryodamage, Cryopreservation, Curcumin, Extender, L Cysteine, Semen

Abstract

The study aimed to evaluate effect of different concentration of cucumin and L cysteine HCL in mitigation of cryopreservation-induced cryodamage in Barbari buck semen. The present experiment was carried out on four Barbari bucks, 2-3 years of age. Twenty-four ejaculates (six from each buck) were collected and diluted with Tris-Egg-Yolk-Citric acid-Fructose-Glycerol extender containing no additives (CON; control), Curcumin 1 and 2μg/ml (CUR1 and CUR2), L cysteine HCL, 2 and 5mM/L (LC2 and LC5) at 37 ºC and cryopreserved following standard protocols, processed, frozen, and evaluated for various seminal markers. As compared to control, the motility (50.50±0.42, 53.50±0.50, 52.50±0.50 and 51.33±0.42%), liveability 77.67±0.98, 81.50±1.05, 81.00±0.96 and 77.67±0.88%) and HOS reactivity (39.83±1.10, 44.00±0.96, 43.00±0.51 and 41.17±0.98%) was significantly (P<0.05) improves, whereas abnormality (11.83±1.07, 7.66±0.55, 8.66±0.49 and 11.00±1.26%) and enzyme leakage i.e. GOT (156.5±6.42, 140.0±5.19, 147.8±4.67, 151.8±5.73μmole/L), GPT (243.7±7.67, 231.5±6.22, 234.2±6.21 and 241.8±6.94 μmole/L), ACP (49.50±2.07, 47.33±1.87, 49.33±1.76 and 51.83±1.85KAU/100 ml) and AKP (140.5±5.28, 134.8±4.76, 137.0±5.02 and 145.5±5.98 KAU/100 ml) significantly decline in CUR1, CUR2, LC2 and LC5 groups respectively.In conclusion,supplementation of 2μg/ml Curcumin and 2mM/L seems to be beneficial in mitigating cryopreservation-induced cryodamagein barbari buck semen.

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