Histochemical and histoenzymic studies on caprine mammary gland following induced Mycoplasma F38 mastitis

Abstract

The histochemical and histoenzymic studies were condueted on mammary glands of 14 lactating goats. The right mammary halves or 12 goats were intracisternally inocualted with 1ml of  Mycoplasma F38 mastitis culture containing a approximately 10" cfu, whereas, their left halves received 1 ml or sterile PPLO broth only,.The remaining 2 goats sreved as unifected controls. All the infected goats developed acute mastitis in their right mammary halves which subsequently turned chronic characterized  by marked decrease in their size and secretions. Histopathologically there ws acute purulent mastitis initially followed by fibroplasia in the infected mammary halves. The histochemical (lipids) and histoenzymic (acid phosphatase, alkaline phosphatase, succinic dehydrogenase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase and glutunic dehydrogenase changes were suggestive of altered fat, carbohydrate and protein metabolism in the infected mammary halves.