In-vitro sperm penetration in polyacrylamide gel to assess frozen semen
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Keywords:
Cervical mucus, Effect of storage, In vitro sperm penetration, Polyacrylamide gel (PAG)Abstract
The feasibility of substituting polyacrylamide gel (PAG) for estrual Bovine Cervical Mucus (BCM) in in-vitro sperm cervical mucus pentration test was assessed. In vitro sperm penetration test for frozen bull spermatozoa were performed using BCM and PAG. Parallel sperm migration in 2% PAG was similar to sperm migration in BCM. The mean sperm penetration distance (SPD) in BCM was 35.36± 1.37 mm and in PAG was 21.47±0.42 mm. The difference was significant (P<0.01). But the bull spermatozoa varying widely in migration distances in BCM maintained a similar ranking in PAG. There was no change in parallel sperm migration distance in the PAG stored at 5°C or at -20°C for lip to 60 days. It was concluded that the polyacrylamide gel can be effectively utilized as a substituted for bovine cervical mucuss in in vitro sperm penetration test and can overcome problem associated with the variability and availability of cervical mucus.Downloads
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The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.
How to Cite
Kumar, R. A., & Devanathan, T. G. (2012). In-vitro sperm penetration in polyacrylamide gel to assess frozen semen. The Indian Journal of Animal Sciences, 68(5). https://epubs.icar.org.in/index.php/IJAnS/article/view/20799