In-vitro sperm penetration in polyacrylamide gel to assess frozen semen


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Authors

  • R Anil Kumar
  • T G Devanathan

Keywords:

Cervical mucus, Effect of storage, In vitro sperm penetration, Polyacrylamide gel (PAG)

Abstract

The feasibility of substituting polyacrylamide gel (PAG) for estrual Bovine Cervical Mucus (BCM) in in-vitro sperm cervical mucus pentration test was assessed. In vitro sperm penetration test for frozen bull spermatozoa were performed using BCM and PAG. Parallel sperm migration in 2% PAG was similar to sperm migration in BCM. The mean sperm penetration distance (SPD) in BCM was 35.36± 1.37 mm and in PAG was 21.47±0.42 mm. The difference was significant (P<0.01). But the bull spermatozoa varying widely in migration distances in BCM maintained a similar ranking in PAG. There was no change in parallel sperm migration distance in the PAG stored at 5°C or at -20°C for lip to 60 days. It was concluded that the polyacrylamide gel can be effectively utilized as a substituted for bovine cervical mucuss in in vitro sperm penetration test and can overcome problem associated with the variability and availability of cervical mucus.

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How to Cite

Kumar, R. A., & Devanathan, T. G. (2012). In-vitro sperm penetration in polyacrylamide gel to assess frozen semen. The Indian Journal of Animal Sciences, 68(5). https://epubs.icar.org.in/index.php/IJAnS/article/view/20799