Effect of estradiol-17 beeta priming on superovulatory response, embryo recovery and progesterone profile in goats

Abstract

The effect of estradiol B priming on day 8 and day 9 of estrous cycle on Superovulatory response, embryo recovery and progesterone proile was studied. Cyclic nondescript goats (16) randomly allocated to control (n=8) and treatment (n=8) groups, received 20 NIH-FSH-SI units in 8 divided doses (4/4.3/3.2/2 and 1/1 units) over 4 days (clay 10 to 13 of the estrous cycle) and 10 mg PGF2a on day 12. In addition. treatment group received subcutaneously estradiol 17ß (0.5 mg in olive oil) injection on day 8 and day 9. Both the groups received an injection of 500 IU human chorionic gonadotrophin, hCG 10-16 hr after onset of estrus  and served naturally at 12 hr interval till the end of estrus. Laparotomy performed on day post 6 of induced oestrus revealed significantly lower numher of corpora lutea (2.00±0.73 vs 5.13±0.93, P<0.05) and significantly higher number of large unovulated follicle (5.50±1.23 vs 2=00±0.90, P<0.05). Number of embryo recovered was low and there was significantly lower progesterone level (5.39± 1.31 vs 10.83±1.05, P<0.05). However, on other days the difference in level of progester· one was statistically nonsignificant. No etfect of estradiol - 17 ß supplementation on superovulation was observed.