Individual variation among buffalo bulls in fertilization and subsequent embryonic development in vitro


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Authors

  • M S Chauhan
  • S K Singla
  • P Palta
  • R S Manik
  • M L Madan

Keywords:

Buffalo bull, In-vitro culture (IVC), In-vitro fertilization (IVF), In-vitro maturation (IVM)

Abstract

Semen from 5 different buffalo bulls was examined to determine whether cleavage rates and development up to blastocyst stage differed as a function of bull used for in vitro fertilization. Buffalo cumulus-oocyte complexes were matured and fertilized in vitro, using frozen-thawed semen capacitated with heparin. The cleavage rates were significantly higher (P<0.05) for bull Nos C and D (49 and 55%, respectively) as compared to those of bull Nos A, B and E (28, 36 and 32% respectively). However, for bull Nos A, B, C, D and E, the proportion of cleaved embryos which developed to morula (45, 53, 52, 53 and 54% respectively) and blastocyst stage (18, 23, 17, 18 and 21% respectively) were not significantly different. These results suggest that there is a variability among buffalo bulls in the fertilizing ability of their spermatozoa.

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How to Cite

Chauhan, M. S., Singla, S. K., Palta, P., Manik, R. S., & Madan, M. L. (2012). Individual variation among buffalo bulls in fertilization and subsequent embryonic development in vitro. The Indian Journal of Animal Sciences, 68(5). https://epubs.icar.org.in/index.php/IJAnS/article/view/20805