Individual variation among buffalo bulls in fertilization and subsequent embryonic development in vitro
82 / 13
Keywords:
Buffalo bull, In-vitro culture (IVC), In-vitro fertilization (IVF), In-vitro maturation (IVM)Abstract
Semen from 5 different buffalo bulls was examined to determine whether cleavage rates and development up to blastocyst stage differed as a function of bull used for in vitro fertilization. Buffalo cumulus-oocyte complexes were matured and fertilized in vitro, using frozen-thawed semen capacitated with heparin. The cleavage rates were significantly higher (P<0.05) for bull Nos C and D (49 and 55%, respectively) as compared to those of bull Nos A, B and E (28, 36 and 32% respectively). However, for bull Nos A, B, C, D and E, the proportion of cleaved embryos which developed to morula (45, 53, 52, 53 and 54% respectively) and blastocyst stage (18, 23, 17, 18 and 21% respectively) were not significantly different. These results suggest that there is a variability among buffalo bulls in the fertilizing ability of their spermatozoa.Downloads
Download data is not yet available.
Downloads
Issue
Section
Articles
License
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.
How to Cite
Chauhan, M. S., Singla, S. K., Palta, P., Manik, R. S., & Madan, M. L. (2012). Individual variation among buffalo bulls in fertilization and subsequent embryonic development in vitro. The Indian Journal of Animal Sciences, 68(5). https://epubs.icar.org.in/index.php/IJAnS/article/view/20805