Experimental rabies in buffalo calves: A pathogenetic approach on early spread of street rabies virus

Abstract

Buffalo calves were experimentlly inoculated with i 25 462 MICLD₅₀ of street rabies virus via linear laceration onthe muzzle. Analysis of tentative progression in quantum and direction of rabies virus indicated that hippocampus,cerebellum, pons and medulla oblongata are much more dependable sites to attempt isolution or rabies virus in buffalospecies especially when a rabid buffalo has been killed during early phase of the disease. On histopathological examination,typical non--suppurative encephalo-myclitis was observed. The central neural transport mechanism of rabies virus hasbeen monitored by specific immunofluorescence,  histopathological findings. Seller's staining and mice inoculation teston various central nervous tissues. Ocular involvement in the form of conjunctivitis was observed among the early symptoms.