Comparative efficacy of enzyme-linked immunosorbent assay (ELISA), dot immunobinding assay (DIA) and indirect haemagglutination test (IHA) for detection of bovine herpesvirus (BHV-l) antibodies in cattle and buffaloes


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Authors

  • K Vasi Reddy
  • T Suri Babu
  • K Varalaxmi
  • M V Subba Rao

Keywords:

Bovine herpesvirus-1, Immunodiagnostic, ELISA, IBR

Abstract

Bovine serum samples (54) from the cases of bovine abortions, respiratory tract infections and apparently healthyanimals were subjected to IHA, ELISA and dot-ELISA tests. In these tests both known positive (BHV -I) and knownnegative serum samples (12 each) were also included. Out of 54 bovine serum samples showed by IHA test, 34 samplesas seropositive ELISA and dot-ELISA all the 54 samples as positive. A comparison was made among ELISA and dot-ELISAwith standard test IHA. ELISA and dot-ELISA were 100% sensitive and specific.

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Issue

Vol. 68 No. 6 (1998)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

Reddy, K. V., Babu, T. S., Varalaxmi, K., & Rao, M. V. S. (2012). Comparative efficacy of enzyme-linked immunosorbent assay (ELISA), dot immunobinding assay (DIA) and indirect haemagglutination test (IHA) for detection of bovine herpesvirus (BHV-l) antibodies in cattle and buffaloes. The Indian Journal of Animal Sciences, 68(6). https://epubs.icar.org.in/index.php/IJAnS/article/view/20891