Multiple anthelmintic resistance in Haemonchus contortus of sheep
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Keywords:Anthelmintic resistance, Egg hatch assay, Faecal egg count reduction, Haemonchus contortus, Sheep
Faecal egg count reduction test (FECRT) and in vitro egg hatch assay (EHA) detected anthelmintic resistance in Haemonchus contortus in sheep. On FECRT, fenbendazole and tetramisole were, respectively, found 0 and 25% effective against H. contortus, while closantel showed 100% efficacy. Though rafoxanide was 96% effective against H. contortus, but lower 95% confidence limit of less than 90 suggested suspected resistance against the rafoxanide. The EHA showed LCâ‚…â‚€ value of 0.265 Â± 0.098 Âµg thiabendazole / ml against H. contortus. It was concluded that H. contortus developed resistance against benzimidazole, tetramisole and low level resistance against rafoxanide.
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