Comparison between RNA electropherotyping and nucleic acid hybridization for the detection of rota virus in faecal samples of diarrhoeic calves
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Keywords:
Nucleic acid hybridization, PCR, RNA electropherotyping, RotavirusAbstract
Gene segment 9 that cuues for VP7 glycoprotein of bovine rotavirus (NCDV strain) was amplified by reverse transcription and polymerase chain reaction. The purified PCR product comprising the full-length cDNA was labelled with DIG-11- dUTP by primer extension method. The sensitivity of the probe was compared with that of polyacrylamide gel electrophoresiss- silver staining (PAGE-SS). The sensitivity of the eDNA probe was 1000- time more than that of PAGE-SS for detection of rotavirus. RNA was extracted from 100 diarrhoeic samples and was tested by PAGE-SS and probe. PAGE-SS revealed 24 samples as positive and probe 58.
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