Freezability and enzyme leakage of crossbred (HF x H) bull semen in 3 dilutors in presence of chlorpromazine HCI

Abstract

Effect of 3 buffers (TRIS, MES and TRICINE) and chlorpromazine HCl as an additive on the freezability of half bred (HFxH) bull spemmtozoa using 48 ejaculates (12 each from 4 bulls) were studied. Motility, livability and enzymes (GOT, GPT, ACP and AKP) were estimated in fresh, prefreeze and post-thaw semen. A significant (P<0.01) variation in motility and livability due to dilutors at prefreeze and post-thaw revealed TRIS as superior buffer than MES and TRICINE. The minimum leakage of enzyme was found in TRIS with chlorpromazine and maximum in TRICINE in absence of chlorpromazine. A significant (P<0.01) improvement in seminal cytomorphological characters and low leakage of spermatozoal enzyme revealed, incorporation of chlorpromazine to be beneficial in achieving better post-thaw recovery rate.