Detection of Theileria annulata using DNA hybridization
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Authors
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K C Roy
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D Ray
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G C Bansal
Keywords:
Digoxigenin, DNA, Dot-blot hybridization, PCR, Ribosomal RNA, Theileria annulataAbstract
A 372 base pair DNA, encoding the small subunit ribosomal RNA of Theileria anllulata, was labelled with a non-radioactive steroid hapten digoxigenin and used to detect the target DNA of T. annulata in dot-blot hybridization assay. The signals of hybridization were recorded in lymphnode biopsy showing macroschizont index up to 0.001 % and infected blood showing intra-erythrocytic parasitaemia up to 0.00062%.
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