Preparation of serum protein isolates from buffalo blood and its quality evaluation

Abstract

Blood was collected from buffalo slaughtered by halal method under hygienic condition. Serum was separated with the help of cream separator. Serum proteins were precipitated with acetone, at different pH (3.0, 5.5, 8.0 and 10.5) and temperatures (4°-5°C and 20°-25°C). The highest and lowest yields were recorded at pH 3.0 at 4°_5°C and 5.5/8.0 at 25°-30° respectively. The total viable count was highest at pH 10.5 and lowest at pH 8.0. The yeast and mould count was highest at pH 5.5 and 4° -5°C and lowest at pH 10.5 and 25°-30°C. Coliform and Enterobacteriaceae count was below 100/g. Salmonella sp. and Staphylococcus were not present in any of the samples. Moisture and ash were lowest in pH 3.0 at 25°-30°C and pH 3.0 at 4°-5°C respectively. Protein was highest at pH 3.0 at 25°-30°C. Lipid was less than 1% in all samples. pH 3.0 at 25°-30°C is recommended for the large-scale production of serum protein isolate from buffalo blood.